The effect of short- and long-term exercise courses on memory impairment induced by ethidium bromide injection in the hippocampus of the brain of male rats

Document Type : Research Paper

Authors
Masome Maleki 1
Homiera Hatami Nemati 1
Hatam Ahmadi 2
Sima Nasri 3
1 Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran

2 Department of Basic Sciences, University of Farhangian, Tehran, Iran

3 Department Biology, Payame Noor university, Tehran, Iran

10.22089/spj.2022.12871.2193
Abstract
Objectives: Multiple sclerosis is the most common non-traumatic debilitating disease that affects the memory of affected people in addition to motor disabilities. The aim of this study was to investigate the effects of improving short- and long-term exercise on spatial memory impairment induced by ethidium bromide injection in the CA region of the hippocampus of male rats.
Materials & Methods: This study was performed on seven groups of 7 male rats with an average age of 10 weeks and a weight range of 200±50 grams, including: control group, sham group, ethidium bromide group, two groups under short and long term exercise and two ethidium bromide + exercise experimental groups. Morris water maze was used to measure spatial memory.
Results: Injection of 3 μl of ethidium bromide 0.01 into the CA1 region of the hippocampus on the third to fifth days of experiments reduced spatial memory-related parameters (P‌ < 0.05). In addition, applying of short and long-term exercise courses was able to improve the parameters of elapsed time (P < 0.05) and distance traveled (P < 0.05) to find the platform in the third to fifth days of the experiment due to the effect of ethidium bromide in the Morris water maze.
Conclusions: Despite the beneficial effect of exercise on improving the memory of rats, the results of the study showed that, short-term exercise course has a greater effect on improving the memory of poisoned rats induced by ethidium bromide injection in a 7-day period than long-term exercise.
Keywords
ethidium bromide
short-term exercise
long-term exercise
spatial memory

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Sport Physiology
Volume 14, Issue 55
December 2022
Pages 71-94

  • Receive Date 11 July 2022
  • Revise Date 14 September 2022
  • Accept Date 02 October 2022