ORIGINAL_ARTICLE
تأثیر شش هفته تمرین تناوبی خیلی شدید بر عوامل منتخب آنژیوژنز قلبی در رت-های مبتلا به آنفارکتوس میوکارد
آنفارکتوس میوکارد، مرگ سلولی غیرقابلبرگشت بخشی از عضلة قلب بهعلت فقدان جریان خون است. فرایند مولکولی افزایش چگالی مویرگی در پاسخ به فعالیت و شدت مناسب فعالیت هنوز معلوم نیست؛ بنابراین، این پژوهش با هدف ارزیابی اثر شش هفته تمرین تناوبی شدید بر بیان ژنهای VEGF و COL-18 و نیز گیرندة مشترک آنها (Flt-1) انجام شد. تعداد 12 سر رت نر نژاد ویستار 10 هفتهای با وزن 250 تا 300 گرم و مبتلابه انفارکتوس میوکارد، در دو گروه تجربی (60 دقیقه دویدن تناوبی روی تردمیل، هر تناوب شامل چهار دقیقه با شدت معادل 90-85 درصد VO2max و دو دقیقه بازیافت فعال با شدت معادل 60-50 درصد VO2max چهار روز در هفته و بهمدت شش هفته) و گروه کنترل (بدون مداخلة تمرین) قرار گرفتند. بیان ژنهای ذکرشده توسط تکنیک PCR بررسی شد. دادهها با استفاده از نرمافزار اس.پی.اس.اس. نسخة 18 (α ≥ 0.05) با روش آماری تی مستقل تجزیهوتحلیل شدند. یافتهها نشان داد که VEGF میوکارد گروه HIITبهطور معناداری بیشتر از گروه کنترل (0.001 ≥P ) و Flt-1 میوکارد در گروه HIIT نیز بهطور معناداری بیشتر از گروه کنترل بود (0.001 ≥ P). COL-18 میوکارد در گروه HIIT نسبت به گروه کنترل تغییر معناداری نداشت (0.340 = P). بهطورکلی، شش هفته تمرین تناوبی شدید باعث افزایش عوامل مؤثر در آنژیوژنز در رتهای نر نژاد ویستار پس از وقوع آنفارکتوس میوکارد میشود.
https://spj.ssrc.ac.ir/article_1732_72cccc507dbaa5536c39fdae90982753.pdf
2019-07-23
17
30
10.22089/spj.2019.2676.1359
آنژیوژنز
آنفارکتوس میوکارد
تمرین تناوبی شدید
سارا
کربلایی فر
sk1980.karbalaei40@yahoo.com
1
دانشجوی دکتری فیزیولوژی ورزشی، پردیس بینالملل کیش، دانشگاه تهران
AUTHOR
عباسعلی
گایینی
aagaeini@yahoo.com
2
استاد فیزیولوژی ورزشی، دانشگاه تهران
AUTHOR
محمد رضا
کردی
mrkordi@ut.ac.ir
3
دانشیار فیزیولوژی ورزشی، دانشگاه تهران
AUTHOR
رضا
نوری
nuri_r7@ut.ac.ir
4
استادیار فیزیولوژی ورزشی، پردیس بینالملل کیش دانشگاه تهران
AUTHOR
پدرام
قربانی
pedramghorbani54@yahoo.com
5
دانشجوی دکتری فیزیولوژی ورزشی، پردیس بینالملل کیش، دانشگاه تهران
AUTHOR
Nordlie MA, Wold LE, Kloner RA. Genetic contributors toward increased risk for ischemic heart disease. J Mol Cell Cardiol. 2005;39(4):667–79.
1
Fukuda S, Kaga S, Sasaki H, Zhan L, Zhu L, Otani H, et al. Angiogenic signal triggered by ischemic stress induces myocardial repair in rat during chronic infarction. J Mol Cell Cardiol. 2004;36:547–59.
2
Nourshahi M, Taheri Chadorneshin H, Ranjbar K. The stimulus of angiogenesis during exercise and physical activity. J HMS. 2013;5:286-96.
3
Gavin TP, Stallings HW, Zwetsloot KA, Westerkamp LM, Ryan NA, Moore RA, et al. Lower capillary density but no difference in VEGF expression in obese v.s. lean young skeletal muscle in humans. J Appl Physiol 2005;98(5):315–23.
4
Prior BM, Yang HT, Terjung RL. What makes vessels grow with exercise training? J Appl Physiol. 2004;97:1119–28.
5
Bates DO. Vascular endothelial growth factors and vascular permeability. Cardiovasc Res. 2010;87:262–71.
6
Mooren F, Völker K. Molecular and cellular exercise physiology. Human Kinetics. 2004:451-7.
7
Heldin CH, Westermark B. Mechanism of action and in vivo role of platelet-derived growth factor. Physiol Rev. 2004;79(4):1283-316.
8
Van Hinsbergh VWM, Koolwijk P. Endothelial sprouting and angiogenesis matrix metalloproteinases in the lead. Cardiovasc Res. 2008;78;203-12.
9
Gu JW, Gadonski G, Wang J, Makey I, Adair TH. Exercise increases endostatin in circulation of healthy volunteers. BMC Physiol. 2004;4(2):1-6.
10
Holloway TM, Bloemberg D, da Silva ML, Simpson JA, Quadrilatero J, Spriet LL. High intensity interval and endurance training have opposing effects on markers of heart failure and cardiac remodeling in hypertensive rats. PLoS One 2015;10(3): 121-38.
11
Laursen PB, Jenkins DG. The scientific basis for high-intensity interval training opti mizing training programmes and maximizing performance in highly trained endurance athletes. Sports Med. 2002;32:53-73.
12
Truijens MJ, Toussaint HM, Dow J, Levine BD. Effect of high-intensity hypoxic training on sea-level swimming performances. J Appl Physiol. 2002;94(2):733-43.
13
Padilla J, Harris RA, Rink LD, WallacePJ. Characterization of the brachial artery shear stress following walking exercise. Vasc Med. 2008;13:105-11.
14
Burgomaster KA, Howarth KR, Phillips SM, Rakobowchuk M, MacDonald MJ, McGee SL, et al. Similar metabolic adaptations during exercise after low volume sprint interval and traditional endurance training in humans. J Physio. 2008;586: 151–60.
15
Rodas G, Ventura JL, Cadefau JA, Cusso R, Parra J. A short training programme for the rapid improvement of both aerobic and anaerobic metabolism. Eur J Appl Physiol. 2000;82:480–6.
16
Edge J, Bishop D, Hill-Haas S, Dawson B, Goodman C. Comparison of muscle buffer capacity and repeated sprint ability of untrained, endurance-trained and team-sport athletes. Eur J Appl Physiol. 2006;96:225–34.
17
Kassia S Weston, Ulrik Wisløff, Jeff S Coombes. High-intensity interval training in patients with lifestyle-induced cardiometabolic disease: A systematic review and meta-analysis. Sports Med. 2014;48:1227–34.
18
Hamzeh Zadeh Brojeni A, Nazar Ali P, Naghibi S. The effect of high intensity interval training (HIIT) on aerobic and anaerobic some indicators of iranian women's national teams of basketball players. Sport Bio. 2012; 5(4):35-48. (In Persian).
19
Martin J, Gibala MG, McGee SL. Metabolic adaptations to short-term high-intensity interval training: A little pain for a lot of gain? Exerce Sport Sci. 2008;36(2):58-63.
20
Buchan DS, Ollis S, Young JD, Thomas NE, Cooper SM, Tong TK, et al. The effects of time and intensity of exercise on novel and established markers of CVD in adolescent youth. Am J Hum Biol. 2011 Jul-Aug;23(4):517-26.
21
O'Donovan G, Owen A, Bird SR, Kearney EM, Nevill AM, Jones DW, et al. Changes in cardiorespiratory fitness and coronary heart disease risk factors following 24 weeks of moderate- or high-intensity exercise of equal energy cost. J Appl Physiol. 2005;98:1619–25.
22
Nazari M, Kordi MR, Choobineh S. The effect of high intensity interval training (HIIT) on Gelatinase-A (MMP-2) serum levels and muscle damage indices in young sedentary. J AMS. 2015;18(94):78-86. (In Persian).
23
Danzig V, Mikova B, Kuchynka P, Benakova H, Zima T, Kittnar O, et al. Levels of circulating biomarkers at rest and after exercise in coronary artery disease patients. Physiol Res. 2010;59(3):385-92.
24
Kraljevic J, Marinovic J, Pravdic D, Zubin P, Dujic Z, Wisloff U, Ljubkovic M. Aerobic interval training attenuates remodelling and mitochondrial dysfunction in the post-infarction failing rat heart. Cardiovasc Res. 2013;99(1):55-64.
25
Morten A, Hoydal MA, Wisloff U, Kemi OJ, Ellingsen O. Running speed and maximal oxygen uptake in rats and mice: practical implications for exercise training. Eur J Cardiovasc Prev Rehabil. 2007;14(6):753-60.
26
Wisloff U, Helgerud J, Kemi OJ, Ellingsen O. Intensity-controlled treadmill running in rats: VO2 max and cardiac hypertrophy. Am J Physiol Heart Circ Physiol. 2000;280(3):301-10.
27
Kemi OJ, Haram PM, Loennechen JP, Osnes JB, Skomedal T, Wisløff U, et al. Moderate vs. high exercise intensity: Differential effects on aerobic fitness, cardiomyocyte contractility, and endothelial function. Cardiovasc Res. 2005;67(1):161-72.
28
Milkiewicz M, Brown MD, Egginton S, Hudlicka O. Association between shear stress, angiogenesis, and VEGF in skeletal muscles in Vivo. Microcirculation. 2001;8:229–41.
29
Koos BJ, Adenosine A. A receptors and O (2) sensing in development. Am J Physiol Regul Integr Comp Physiol. 2011;30(1):601-22.
30
Hudlicka O, Brown MD. Adaptation of skeletal muscle microvasculature to increased or decreased blood flow role of shear stress nitric oxide and vascular endothelial growth factor. J Vasc Res. 2009;46:504–12.
31
Ribatti D, Crivellato E. Mast cells, angiogenesis, and tumor growth. Biochim Biophys Acta. 2012;1822(1):2-8.
32
Folkman J. Fundamental concepts of the angiogenic process. Curr Mol Med. 2003;3(7):643-51.
33
ORIGINAL_ARTICLE
تأثیر شش هفته تمرین استقامتی بر بیان ژنهای آپوپتوزی و ضدآپوپتوزی بطن چپ قلب موشهای صحرایی نر سالمند
هدف پژوهش حاضر، بررسی تأثیر شش هفته تمرین استقامتی تداومی بر بیان ژن کاسپاز-3 و متالوتیونئین-2 بافت بطن چپ موشهای صحرایی نر سالمند بود. تعداد 14 سر موش صحرایی (26-24 ماهه و میانگین وزنی 20 ± 380 گرم)، بهطور تصادفی به دو گروه کنترل و تمرین تقسیم شدند. گروه تمرین، تمرین استقامتی تداومی روی تردمیل را بهمدت شش هفته انجام دادند ( 65 تا 70 درصد سرعت بیشینه).بیان ژنهای کاسپاز-3 و متالوتیونئین- 2 بافت بطن چپ با تکنیک Real time PCR سنجیده شد و پس از کمیسازی مقادیر بیان ژن، با استفاده از فرمول 2-∆∆ct بهوسیلة روش آماری تی مستقل بررسی شد (P < 0.05). نتایج نشان داد که شش هفته تمرین استقامتی تداومی به کاهش غیرمعنادار بیان ژن کاسپاز-3 (P = 0.078) و افزایش غیرمعنادار بیان ژن متالوتیونئین-2 (P = 0.513) منجر شد. بهنظر میرسد که انجام تمرینهای استقامتی تداومی در سالمندان خطر آسیبهای ناشی از آپوپتوز را القا نمیکند و میتواند در این جمعیت بهکار گرفته شود.
https://spj.ssrc.ac.ir/article_1423_c8f5f2deec8a7b97cb26ef8ed686bc85.pdf
2019-07-23
31
46
10.22089/spj.2018.4865.1656
کاسپاز-3
متالوتیونئین-2
بافت بطن چپ
فعالیت استقامتیتداومی
آپوپتوز
عاطفه
احمدنیا
ahmadniya63@yahoo.com
1
کارشناسیارشد فیزیولوژی ورزشی، دانشگاه پیام نورکرج
LEAD_AUTHOR
محمد
فشی
m_fashi@sbu.ac.ir
2
استادیار فیزیولوژی ورزشی، دانشگاه شهید بهشتی تهران
AUTHOR
محمد رضا
اسد
m_r_asad@yahoo.com
3
دانشیار فیزیولوژی ورزشی، دانشگاه پیام نور
AUTHOR
Wheeler HE, Kim SK. Genetics and genomics of human ageing. Philosophical Transactions of the Royal Society of London B: Biological Sciences. 2011; 366(1561):43-50.
1
Tacutu R, Craig T, Budovsky A, Wuttke D, Lehmann G, Taranukha D, Costa J, Fraifeld VE, De Magalhães JP. Human ageing genomic resources: Integrated databases and tools for the biology and genetics of ageing. Nucleic acids Research. 2012;41(D1): 1027-33
2
Vina J, Borras C, Miquel J. Theories of ageing. IUBMB life. 2007; 59(4‐5): 249-54.
3
Rattan SI. Increased molecular damage and heterogeneity as the basis of aging. Biological Chemistry. 2008;389(3):267-72.
4
Romano AD, Serviddio G, de Matthaeis A, Bellanti F, Vendemiale G. Oxidative stress and aging. Journal of Nephrology. 2010;23: 29-36.
5
Burgoyne JR, Mongue-Din H, Eaton P, Shah AM. Redox signaling in cardiac physiology and pathology. Circulation Research. 2012;111(8):1091-106.
6
Fiechter M, Fuchs TA, Gebhard C, Stehli J, Klaeser B, Stähli BE, et al. Age-related normal structural and functional ventricular values in cardiac function assessed by magnetic resonance. BMC medical imaging. 2013;13(1):2-6.
7
Khoshtabiat L, Mahdavi M. The role of oxidative stress in proliferation and cell death. Journal of Mazandaran University of Medical Sciences. 2015;25(127):130-45. (In Persian).
8
Afzalpour ME, Gharakhanlou R, Gaeini AA, Mohebbi H, Hedayati M, Khazaei M. The effects of aerobic exercises on the serum oxidized LDL and total antioxidant capacity in non-active men. CVD prevention and control. 2008;3(2):77-82.
9
Naghizadeh H, Banparvari M, Salehikia A. Effect of one course exercise with consumption vitamin E on antioxidant status and cardiovascular risk factors. Zahedan Journal of Research in Medical Sciences. 2010, 1;12(1):32-39.
10
Dröge W. Free radicals in the physiological control of cell function. Physiological reviews. 2002;82(1):47-95.
11
Jiang X, Wang X. Cytochrome C-mediated apoptosis. Annual review of biochemistry. 2004;73:87-106.
12
Hepple RT. Impact of aging on mitochondrial function in cardiac and skeletal muscle. Free Radical Biology and Medicine. 2016;98:177-86.
13
Kwak HB, Kim JH, Joshi K, Yeh A, Martinez DA, Lawler JM. Exercise training reduces fibrosis and matrix metalloproteinase dysregulation in the aging rat heart. The FASEB Journal. 2011;25(3):1106-17.
14
Kwak HB. Effects of aging and exercise training on apoptosis in the heart. Journal of exercise rehabilitation. 2013;9(2):212-9.
15
Kanter M, Aksu F, Takir M, Kostek O, Kanter B, Oymagil A. Effects of low intensity exercise against apoptosis and oxidative stress in streptozotocin-induced diabetic rat heart. Exp Clin Endocrinol Diabetes 2017; 125(09): 583-91.
16
Rahimi M, Asgari AR, Khoshbaten A. The role of exercise preconditioning in cardioprotection against ischemia reperfusion injury. Physiology and Pharmacology. 2014;18(2):122-43. (In Persian).
17
Ulbrich AZ, Angarten VG, Netto AS, Sties SW, Bündchen DC, de Mara LS, et al. Comparative effects of high intensity interval training versus moderate intensity continuous training on quality of life in patients with heart failure: study protocol for a randomized controlled trial. Clinical Trials and Regulatory Science in Cardiology. 2016;13:21-8.
18
Lamprecht M. Antioxidants in sport nutrition. Boca Raton, CRC Press; 1st Edition, 2014.
19
Siu PM, Bryner RW, Martyn JK, Alway SE. Apoptotic adaptations from exercise training in skeletal and cardiac muscles. The FASEB Journal. 2004;18(10): 1150-2.
20
Zhang JH, Zhang Y, Herman B. Caspases, apoptosis and aging. Ageing research reviews. 2003;2(4):357-66.
21
Creagh, E. M., and S. J. Martin. "Caspases: cellular demolition experts. Biochemical Society Transactions". 2001. 29(6): 696-702.
22
Konstantinidis K, Whelan RS, Kitsis RN. Mechanisms of cell death in heart disease. Arteriosclerosis, thrombosis, and vascular biology. 2012;32(7):155262.
23
Dirks A, Leeuwenburgh C. Apoptosis in skeletal muscle with aging. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 2002;282(2):519-27.
24
Burgess DH, Svensson M, Dandrea T, Grönlund K, Hammarquist F, Orrenius S, Cotgreave IA. Human skeletal muscle cytosols are refractory to cytochrome c-dependent activation of type-II caspases and lack APAF-1. Cell Death & Differentiation. 1999;6(3): 251-61.
25
Pollack M, Phaneuf S, Dirks A, Leeuwenburgh C. The role of apoptosis in the normal aging brain, skeletal muscle, and heart. Annals of the New York Academy of Sciences. 2002;959(1):93-107.
26
Sandri M, Carraro U, Podhorska-Okolov M, Rizzi C, Arslan P, Monti D, et al. Apoptosis, DNA damage and ubiquitin expression in normal and mdx muscle fibers after exercise. FEBS letters. 1995;373(3):291-5.
27
Urbanek K, Quaini F, Tasca G, Torella D, Castaldo C, Nadal-Ginard B, et al. Intense myocyte formation from cardiac stem cells in human cardiac hypertrophy. Proceedings of the National Academy of Sciences. 2003;100(18):10440-5.
28
Lakatta EG. Why cardiovascular function may decline with age. Geriatrics. 1987; 42(6):84-7, 91-4.
29
Zalewska M, Trefon J, Milnerowicz H. The role of metallothionein interactions with other proteins. Proteomics. 2014;14(11):1343-56.
30
Gurel Z, Ozcelik D, Dursun S. Apoptotic rate and metallothionein levels in the tissues of cadmium-and copper-exposed rats. Biological Trace Element Research. 2007;116(2):203-17.
31
Capdevila M, Atrian S. Metallothionein protein evolution: A miniassay. Journal of Biological Inorganic Chemistry. 2011;16(7):977-89
32
Jing L, Li L, Zhao J, Zhao J, Sun Z, Peng S. Zinc-induced metallothionein overexpression prevents doxorubicin toxicity in cardiomyocytes by regulating the peroxiredoxins. Xenobiotica. 2016;46(8):715-25.
33
Zhang Y, Li L, Hua Y, Nunn JM, Dong F, Yanagisawa M, et al. Cardiac-specific knockout of ETA receptor mitigates low ambient temperature-induced cardiac hypertrophy and contractile dysfunction. Journal of Molecular Cell Biology. 2012;4(2):97-107.
34
Sinha RP, Häder DP. UV-induced DNA damage and repair: A review. Photochemical & Photobiological Sciences. 2002;1(4):225-36.
35
Fashi M, Agha-Alinejad H, Mahabadi HA, Rezaei B, Pakrad BB, Rezaei S. The effects of aerobic exercise on NF-κB and TNF-α in lung tissue of male rat. Novelty in Biomedicine. 2015;3(3):131-4.
36
Thomas C, Bishop D, Moore-Morris T, Mercier J. Effects of high-intensity training on MCT1, MCT4, and NBC expressions in rat skeletal muscles: influence of chronic metabolic alkalosis. American Journal of Physiology-Endocrinology and Metabolism. 2007;293(4):916-22.
37
Burniston JG. Adaptation of the rat cardiac proteome in response to intensity‐controlled endurance exercise. Proteomics. 2009;9(1):106-15.
38
Rodrigues B, Figueroa DM, Mostarda CT, Heeren MV, Irigoyen MC, De Angelis K. Maximal exercise test is a useful method for physical capacity and oxygen consumption determination in streptozotocin-diabetic rats. Cardiovascular diabetology. 2007;6(1):38-49.
39
Yuan JS, Reed A, Chen F, Stewart CN. Statistical analysis of real-time PCR data. BMC bioinformatics. 2006;7(1):85-96.
40
Kwak HB, Song W, Lawler JM. Exercise training attenuates age-induced elevation in Bax/Bcl-2 ratio, apoptosis, and remodeling in the rat heart. The FASEB Journal. 2006;20(6):791-3.
41
Putri M. The effects of aerobic exercise and detraining on left ventricular cardiomyocyte apoptosis. YARSI Medical Journal. 2017;24(3):157-65.
42
Fang CX, Doser TA, Yang X, Sreejayan N, Ren J. Metallothionein antagonizes aging‐induced cardiac contractile dysfunction: role of PTP1B, insulin receptor tyrosine phosphorylation and Akt. Aging Cell. 2006;5(2):177-85.
43
Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell. 1997;91(4):479-89.
44
Podhorska-Okołów M, Dziegiel P, Dolińska-Krajewska B, Dumańska M, Cegielski M, Jethon Z, et al. Expression of metallothionein in renal tubules of rats exposed to acute and endurance exercise. Folia Histochemica et Cytobiologica. 2006;44(3):195-200.
45
Jolazadeh, T, Dabidi Roshan, V, Mirdar. Sh. Apoptosis in cardiac cells in response to acute exercise protocol: journal of applied exercise physiology. 2010;6(11):27-36. (In Persian).
46
Shabani M, Sherafati Moghadam M, Daryanoosh F. Effect of four weeks high intensity interval training versus aerobic exercise on metallothionein levels of myocardial tissue in rats. Journal of Sabzevar University of Medical Sciences. 2016;3(86):14-9. (In Persian).
47
Chen TI, Chen MY. Zinc is indispensable in exercise-induced cardioprotection against intermittent hypoxia-induced left ventricular function impairment in rats. PloS one. 2016;11(12):168600.
48
Wang GW, Zhou Z, Klein JB, Kang YJ. Inhibition of hypoxia/reoxygenation-induced apoptosis in metallothionein-overexpressing cardiomyocytes. American Journal of Physiology-Heart and Circulatory Physiology. 2001;280(5):2292-9.
49
ORIGINAL_ARTICLE
فعالنشدن مسیر پیامرسانی MAPK عضلة اسکلتی مردان غیرفعال با پیادهروی حاد تناوبی همراه با محدودیت جریان خون
هدف از انجام پژوهش حاضر، بررسی تأثیر پیادهروی حاد تناوبی با محدودیت جریان خون بر غلظت پروتئینهای 4EBP1، ERK و P38 عضلة اسکلتی مردان غیرفعال بود. پنج مرد غیرفعال سالم در دو جلسه پیادهروی حاد تناوبی با محدودیت جریان خون (پنج وهلة سهدقیقهای و یک دقیقه استراحت بین هر وهله با 55 درصد بیشینة ضربان قلب همراه با فشار شریانبند از فشار اولیة 120 به 160 میلیمتر جیوة بالای عضله چهارسرران) و پیادهروی حاد تناوبی بدون محدودیت جریان خون (پنج وهلة سهدقیقهای و یک دقیقه استراحت بین هر وهله با 55 درصد بیشینة ضربان قلب) شرکت کردند. نمونهبرداری از عضلة پهن خارجی ران در قبل و سه ساعت پس از تمرین از پای راست آزمودنیها، با فاصلة پنج تا 10 سانتی از هم انجام گرفت. میزان پروتئینهای 4EBP1، ERK و P38 بهوسیلة تکنیک وسترن بلات بررسی شد. از آزمونهای تی وابسته و تی مستقل پس از کسر نمرة پسآزمون از پیشآزمون، برای تحلیل دادههای هر دو گروه استفاده شد (P ≤ 0.05). نتایج آزمون تی وابسته تفاوت معناداری را بین پیشآزمون و پسآزمون4EBP1 (0.001= P) و ERK( 0.049=P ) و نبود تفاوت معناداری را در P38 (0.452 = P) در گروه پیادهروی تناوبی با محدودیت جریان خون نشان داد. تفاوت معناداری نیز بین پیشآزمون و پسآزمون ERK (0.012 = P) و نبود تفاوت معنادار4EBP1 (0.064 =P ) و P38( 0.122=P ) در گروه پیادهروی تناوبی بدون محدودیت جریان خون مشاهده شد. نتایج آزمون تی مستقل تفاوت معناداری را بین دو شیوة تمرینی برای متغیرهای4EBP1 (0.068 =P )،ERK (0.091 = P) و P38(0.827 = P) نشان نداد (P ≥ 0.05). نتایج پژوهش نشان داد که پیادهروی حاد تناوبی همراه با محدودیت جریان خون مسیر پیامرسانی MAPK را فعال نمیکند.
https://spj.ssrc.ac.ir/article_1526_ae75a4afd4ba941e567bf05b917cc11a.pdf
2019-07-23
47
60
10.22089/spj.2018.5839.1768
تمرین پیادهروی
محدودیت جریان خون
هایپرتروفی
سنتز پروتئین
مهدی
خوبی
mehdi.khoubi@yahoo.com
1
دانشجوی دکترای فیزیولوژی ورزشی، دانشگاه شهید چمران اهواز، عضو هیات علمی دانشگاه پیام نور
LEAD_AUTHOR
عبدالحمید
حبیبی
hamidhabibi330@gmail.com
2
استاد فیزیولوژی ورزشی، دانشگاه شهید چمران اهواز
AUTHOR
محسن
قنبرزاده
ghanbarzadeh213@gmail.com
3
استادیار فیزیولوژی ورزشی، دانشگاه شهید چمران اهواز
AUTHOR
سعید
شاکریان
sashakeryan@gmail.com
4
دانشیار فیزیولوژی ورزشی، دانشگاه شهید چمران اهواز
AUTHOR
بهمن
میرزایی
bmirzaei2000@yahoo.com
5
استاد فیزیولوژی ورزشی، دانشگاه گیلان
AUTHOR
Hawley JA. Molecular responses to strength and endurance training: are they incompatible? Applied Physiology, Nutrition, and Metabolism. 2009;34(3):355-61.
1
Bassett Jr DR, Howley ET. Limiting factors for maximum oxygen uptake and determinants of endurance performance. Medicine & Science in Sports & Exercise. 2000;32(1):70-4.
2
Nader GA. Concurrent strength and endurance training: from molecules to man. Medicine and Science in Sports and Exercise. 2006;38(11):1965-9.
3
Abe T, Fujita S, Nakajima T, Sakamaki M, Ozaki H, Ogasawara R, et al. Effects of low-intensity cycle training with restricted leg blood flow on thigh muscle volume and VO2max in young men. Journal of Sports Science & Medicine. 2010;9(3): 452-7.
4
Abe T, Yasuda T, Midorikawa T, Sato Y, CF K, Inoue K, et al. Skeletal muscle size and circulating IGF-1 are increased after two weeks of twice daily “KAATSU” resistance training. International Journal of KAATSU Training Research. 2005;1(1):6-12.
5
Abe T, Sakamaki M, Fujita S, Ozaki H, Sugaya M, Sato Y, et al. Effects of low‐intensity walk training with restricted leg blood flow on muscle strength and aerobic capacity in older adults. Journal of Geriatric Physical Therapy. 2010;33(1):34-40.
6
Pope ZK, Willardson JM, Schoenfeld BJ. Exercise and blood flow restriction. The Journal of Strength & Conditioning Research. 2013;27(10):2914-26.
7
Park S, Kim JK, Choi HM, Kim HG, Beekley MD, Nho H. Increase in maximal oxygen uptake following 2-week walk training with blood flow occlusion in athletes. European Journal of Applied Physiology. 2010;109(4):591-600.
8
Bickel CS, Slade J, Mahoney E, Haddad F, Dudley GA, Adams GR. Time course of molecular responses of human skeletal muscle to acute bouts of resistance exercise. Scandinavian Journal of Medicine & Science in Sports. 2005;15(2):135-6.
9
Pearson SJ, Hussain SR. A review on the mechanisms of blood-flow restriction resistance training-induced muscle hypertrophy. Sports Medicine. 2015;45(2): 187-200.
10
Yasuda T, Fujita S, Ogasawara R, Sato Y, Abe T. Effects of low‐intensity bench press training with restricted arm muscle blood flow on chest muscle hypertrophy: a pilot study. Clinical Physiology and Functional Imaging. 2010;30(5):338-43.
11
Abe T, Kearns CF, Sato Y. Muscle size and strength are increased following walk training with restricted venous blood flow from the leg muscle, Kaatsu-walk training. Journal of Applied Physiology. 2006;100(5):1460-6.
12
Mangine GT, Hoffman JR, Gonzalez AM, Townsend JR, Wells AJ, Jajtner AR, et al. The effect of training volume and intensity on improvements in muscular strength and size in resistance‐trained men. Physiological Reports. 2015;3(8): 12472-6.
13
Inoki K, Li Y, Zhu T, Wu J, Guan K-L. TSC2 is phosphorylated and inhibited by Akt and suppresses mTOR signalling. Nature Cell Biology. 2002;4(9):648-56.
14
Mahoney SJ, Dempsey JM, Blenis J. Cell signaling in protein synthesis: ribosome biogenesis and translation initiation and elongation. Progress in Molecular Biology and Translational Science. 2009;90:53-107.
15
Ozaki H, Kakigi R, Kobayashi H, Loenneke J, Abe T, Naito H. Effects of walking combined with restricted leg blood flow on mTOR and MAPK signalling in young men. Acta Physiologica. 2014;211(1):97-106.
16
O’Neil TK, Duffy L, Frey J, Hornberger T. The role of phosphoinositide 3‐kinase and phosphatidic acid in the regulation of mammalian target of rapamycin following eccentric contractions. The Journal of Physiology. 2009;587(14):3691-701.
17
Drummond MJ, Fujita S, Takashi A, Dreyer HC, Volpi E, Rasmussen BB. Human muscle gene expression following resistance exercise and blood flow restriction. Medicine and Science in Sports and Exercise. 2008;40(4):691-8.
18
Gundermann DM, Walker DK, Reidy PT, Borack MS, Dickinson JM, Volpi E, et al. Activation of mTORC1 signaling and protein synthesis in human muscle following blood flow restriction exercise is inhibited by rapamycin. American Journal of Physiology-Endocrinology and Metabolism. 2014;306(10):1198-204.
19
Fry CS, Glynn EL, Drummond MJ, Timmerman KL, Fujita S, Abe T, et al. Blood flow restriction exercise stimulates mTORC1 signaling and muscle protein synthesis in older men. Journal of Applied Physiology. 2010;108(5):1199-209.
20
Freitas ED, Poole C, Miller RM, Heishman AD, Kaur J, Bemben DA, et al. Time course change in muscle swelling: High-intensity vs. blood flow restriction exercise. International Journal of Sports Medicine. 2017;38(13):1009-16.
21
Nygren A, Sundberg C, Esbjörnsson-Liljedahl M, Jansson E, Kaijser L. Effects of dynamic ischaemic training on human skeletal muscle dimensions. European Journal of Applied Physiology. 2000;82(1-2):137-41.
22
Harber MP, Konopka AR, Douglass MD, Minchev K, Kaminsky LA, Trappe TA, et al. Aerobic exercise training improves whole muscle and single myofiber size and function in older women. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 2009;297(5):1452-9.
23
Scott BR, Loenneke JP, Slattery KM, Dascombe BJ. Exercise with blood flow restriction: An updated evidence-based approach for enhanced muscular development. Sports Medicine. 2015;45(3):313-25.
24
McCarthy JP, Agre JC, Graf BK, Pozniak MA, Vailas AC. Compatibility of adaptive responses with combining strength and endurance training. Medicine and Science in Sports and Exercise. 1995;27(3):429-36.
25
Bell G, Syrotuik D, Martin T, Burnham R, Quinney H. Effect of concurrent strength and endurance training on skeletal muscle properties and hormone concentrations in humans. European Journal of Applied Physiology. 2000;81(5):418-27.
26
Kraemer WJ, Patton JF, Gordon SE, Harman EA, Deschenes MR, Reynolds K, et al. Compatibility of high-intensity strength and endurance training on hormonal and skeletal muscle adaptations. Journal of Applied Physiology. 1995;78(3):976-89.
27
Fujita S, Abe T, Drummond MJ, Cadenas JG, Dreyer HC, Sato Y, et al. Blood flow restriction during low-intensity resistance exercise increases S6K1 phosphorylation and muscle protein synthesis. Journal of Applied Physiology. 2007;103(3):903-10.
28
Renzi CP, Tanaka H, Sugawara J. Effects of leg blood flow restriction during walking on cardiovascular function. Medicine and Science in Sports and Exercise. 2010;42(4):726.
29
ORIGINAL_ARTICLE
تأثیر شدت های مختلف تمرین مقاومتی و استقامتی بر بیان microRNA-133a و دو فاکتور نسخهبرداری استئوژنز و آدیپوژنز Runx2 و PPARγ در مغز استخوان موشهای صحرایی نر سالمند نژاد ویستار
هدف از انجام مطالعة حاضر، تأثیر شدتهای مختلف تمرین مقاومتی و استقامتی بر بیان microRNA-133a (mir-133a)، RUNX2 و گیرندة فعالکنندة تکثیر پروکسی زوم گاما (PPARγ) در مغز اسـتخوان موشهای صحرایی نر سالمند نژاد ویستار بود. 40 سر موش صحرایی نر سالمند (میانگین سنی: 23 ماه، میانگین وزن: 27/438 گرم) نژاد ویستار به پنج گروه مساوی هشتتایی شامل تمرینهای مقاومتی و استقامتی با شدت متوسط و شدید و گروه کنترل بهطور تصادفی تقسیم شدند. تمرینهای مقاومتی متوسط و شدید بهترتیب با شدت 60 درصد و 80 درصد حداکثر ظرفیت حمل ارادی با استفاده از نردبان مقاومتی، تمرین استقامتی با شدت متوسط 70-60 درصد سرعت بیشینه و تمرین استقامتی با شدت شدید 110-80 درصد سرعت بیشینه، با استفاده از تردمیل جوندگان انجام شدند و هر دو نوع تمرین پنج روز در هفته بهمدت هشتهفته انجام شدند. بعد از پایان دورة تمرین، بیان mir-133a ، RUNX2 و PPARγ در بافت مغز استخوان تیبیا به روش RT–PCR اندازهگیری شد. تجزیهوتحلیل آماری با استفاده از آزمون کروسکال والیس در سطحP ≤ 0.05 انجام شد. این پژوهش نشان داد که میزان بیان mir-133a0.197) = P)، Runx2 (0.960 =P ) و PPARγ0.872) = P) در گروههای تمرینی با شدت بالا و متوسط در مقایسه با گروه کنترل تفاوت معناداری نداشت؛ بنابراین، بهنظر میرسد که تغییرات سلولی متابولیسم استخوان پس از انجام چنین تمرینهایی به دورههای طولانیتر تمرین ورزشی یا اندازهگیریهای دیگری بهویژه در سطح پروتئومیکس نیاز دارد که باید در پژوهشهای آینده بررسی شود.
https://spj.ssrc.ac.ir/article_1527_f469e531220f36ab1b957d711fe6b690.pdf
2019-07-23
61
78
10.22089/spj.2018.6130.1791
mir-133a
Runx2
PPARγ
تمرین استقامتی و مقاومتی
متابولیسم استخوان
زهرا
همتی فارسانی
hematyn.sport87@yahoo.com
1
دانشجوی دکتری فیزیولوژی ورزشی، دانشگاه شهرکرد، شهرکرد، ایران
LEAD_AUTHOR
ابراهیم
بنی طالبی
banitalebi.e@gmail.com
2
دانشیار فیزیولوژی ورزشی، دانشگاه شهرکرد، شهرکرد، ایران
AUTHOR
محمد
فرامرزی
md.faramarzi@gmail.com
3
دانشیار فیزیولوژی ورزشی، دانشگاه شهرکرد، شهرکرد، ایران
AUTHOR
امین
بی غم صادق
dr.bigham@gmail.com
4
استاد علوم درمانگاهی، دانشگاه شهرکرد، شهرکرد، ایران
AUTHOR
Qi Z, Liu W, Lu J. The mechanisms underlying the beneficial effects of exercise on bone remodeling: Roles of bone-derived cytokines and microRNAs. Prog Biophys Mol. 2016;122(2):131-9.
1
Rosenthall L, Falutz J, Guaraldi G. The relationships between total body, lumbar spine and femoral neck bone mineral density T-scores for diagnosis of low bone mass in HIV-infected patients. J Clin Nutr Metab. 2018;1:2-5.
2
Tuck SP, Datta HK. Osteoporosis in the aging male: Treatment options. Clin Interv Aging. 2007;2(4):521-36.
3
Chen Q, Shou P, Zheng C, Jiang M, Cao G, Yang Q, et al. Fate decision of mesenchymal stem cells: Adipocytes or osteoblasts. Cell Death Differ. 2016;23(7):1128-39.
4
Delaine-Smith RM, Reilly GC. Mesenchymal stem cell responses to mechanical stimuli. Muscles Ligaments Tendons J. 2012;2(3):169-80.
5
Fahy N, Alini M, Stoddart MJ. Mechanical stimulation of mesenchymal stem cells: Implications for cartilage tissue engineering. J Orthop Res. 2018;36(1):52-63.
6
Marędziak M, Śmieszek A, Chrząstek K, Basinska K, Marycz K. Physical activity increases the total number of bone-marrow-derived mesenchymal stem cells, enhances their osteogenic potential, and inhibits their adipogenic properties. Stem Cills Int. 2015;2015: 1-15
7
Liu SY, Li Z, Xu SY, Xu L, Yang M, Ni GX. Intensity‑dependent effect of treadmill running on differentiation of rat bone marrow stromal cells. Mol Med Rep. 2018;17(6):7746-56.
8
Hell RCR, Ocarino NM, Boeloni JN, Silva JF, Goes AM, Santos R L. Physical activity improves age‐related decline in the osteogenic potential of rats' bone marrow‐derived mesenchymal stem cells. Acta Physiol. 2012;205(2):292-301.
9
Franceschi RT, Xiao G. Regulation of the osteoblast‐specific transcription factor, Runx2: Responsiveness to multiple signal transduction pathways. J Cell Biochem. 2003;88(3):446-54.
10
Zhang Y, Khan D, Delling J, Tobiasch E. Mechanisms underlying the osteo-and adipo-differentiation of human mesenchymal stem cells. Sci World J. 2012;2012: 1-14
11
Yuan Y, Zhang L, Tong X, Zhang M, Zhao Y, Guo J, et al. Mechanical stress regulates bone metabolism through micrornas. J Cell Physiol. 2017;232(6):1239-45.
12
Mohan S, Wergedal JE, Das S, Kesavan C. Conditional disruption of miR17-92 cluster in collagen type I-producing osteoblasts results in reduced periosteal bone formation and bone anabolic response to exercise. Phsiyol Genomics. 2014;47(2): 33-43.
13
Zuo B, Zhu JF, Li J, Wang CD, Zhao XY, Cai GQ, et al. MicroRNA‐103a functions as a mechanosensitive microRNA to inhibit bone formation through targeting runx2. J Bone Miner Res. 2015;30(2):330-45.
14
Wang H, Sun Z, Wang Y, Hu Z, Zhou H, Zhang L, et al. MiR-33-5p, a novel mechano-sensitive microRNA promotes osteoblast differentiation by targeting Hmga2. Sci Rep-UK. 2016;6:23170-85.
15
Bailey C, Brooke-Wavell K. Exercise for optimising peak bone mass in women: Postgraduate Symposium. P Nutr Soc. 2008;67(1):9-18.
16
Kiuchi A, Shimegi S, Tanaka I, Izumo N, Fukuyama R, Nakamuta H, et al. Dose-response effects of exercise intensity on bone in ovariectomized rats. Int J Sport Health Sci. 2006;4:10-8.
17
Song F, Jiang D, Wang T, Wang Y, Lou Y, Zhang Y. Mechanical stress regulates osteogenesis and adipogenesis of rat mesenchymal stem cells through PI3K/Akt/GSK-3β/β-catenin signaling pathway. Biomed Res Int. 2017; 2017: 6027402-10
18
Gregov C, Šalaj S. The Effects of Different training modalities on bone mass: a Review. Kinesiol. Int J Fundam Appl Kinesiol. 2014;46(Supplement 1):10-29.
19
Kohrt WM, Bloomfield SA, Little KD, Nelson ME, Yingling VR. Physical activity and bone health. Med Sci Sports Exerc. 2004;36(11):1985-96.
20
Turner CH, Robling AG. Mechanisms by which exercise improves bone strength. J Bone Miner Metab. 2005;23(1):16-22.
21
Markou KB, Mylonas P, Theodoropoulou A, Kontogiannis A, Leglise M, Vagenakis AG, et al. The influence of intensive physical exercise on bone acquisition in adolescent elite female and male artistic gymnasts. J Endocrinol Metab. 2004;89:4383–7.
22
Maddalozzo GF, Snow CM. High intensity resistance training effects on bone in older men and women. Calcif Tissue Int. 2000;66:399–404.
23
de Cassia Marqueti R, Almeida JA, Guzzoni V, Boghi F, Renner A, Silva PE, et al. Resistance training minimizes the biomechanical effects of aging in three different rat tendons. J Biomech. 2017;53:29-35.
24
Krug AL, Macedo AG, Zago AS, Rush JWE, Santos CF, Amaral SL. High-intensity resistance training attenuates dexamethasone-induced muscle atrophy. Muscle Nerve. 2016;53(5):779-88.
25
Macedo AG, Krug ALO, Herrera NA, Zago AS, Rush JWE, Amaralab SL. Low-intensity resistance training attenuates dexamethasone-induced atrophy in the flexor hallucis longus muscle. J Steroid Biochem Mol Biol. 2014;143:357-64.
26
Leandro CG, Levada AC, Hirabara SM, Manhães-de-Castro R, De-Castro CB, Curi R, et al. aprogram of moderate physical training for wistar rats based on maximal oxygen consumption. J Strength Cond Res. 2007;21(3):751-6.
27
Rezaei R, Norshahi M, Bigdeli M, Khodagholi F, Haghparast A. Effect of eight weeks continues and HIIT exercises on VEGF-A and VEGFR-2 levels in stratum, hippocampus and cortex of wistar rat brain. J PHYSIO sport PA. 2016;(16):1213-21. (In Persian).
28
Fani F, Abbassi Daloii A, Abdi A. The effect of 8 weeks of endurance training and L-NAME on Apelin in adipose tissue in elderly male’s rats. J practi studi bio sport. 2016;4(8):77-88. (In Persian).
29
Kim SH, Kim GJ, Umemura T, Lee SG, Cho KJ. Aberrant expression of plasma microRNA-33a in an atherosclerosis-risk group. Mol Biol Rep. 2017;44(1):79-88.
30
Soves CP, Miller JD, Begun DL, Taichman RS, Hankenson KD, Goldsteina SA. Megakaryocytes are mechanically responsive and influence osteoblast proliferation and differentiation. Bone. 2014;66:111-20.
31
Nie Y, Sato Y, Wang C, Yue F, Kuang S, Gavin TP. impaired exercise tolerance, mitochondrial biogenesis, and muscle fiber maintenance in miR-133a–deficient mice. FASEB Journal. 2016;30(11):3745-58.
32
Gomes CPC, Oliveira-Jr GP, Madrid B, Almeida JA, Franco OL, Pereira RW. Circulating miR-1, miR-133a, and miR-206 levels are increased after a half-marathon run. Biomarkers. 2014;19(7):585-9.
33
Nielsen S, Scheele C, Yfanti C, Åkerström T, Nielsen AR, Pedersen BK, et al. Muscle specific microRNAs are regulated by endurance exercise in human skeletal muscle. J Physiol. 2010;588(20):4029-37.
34
Ogasawara R, Akimoto T, Umeno T, Sawada S, Hamaoka T, Fujita S. MicroRNA expression profiling in skeletal muscle reveals different regulatory patterns in high and low responders to resistance training. Physiol Genomics. 2016;48(4):320-4.
35
Li M, Bai Y, Jianfei C, Xiaodong X, Yuanyuan D, Jing Z. Effects of different exercise intensity on PPARγ and relative index in adolescent obesity rats. Wei Sheng Yan Jiu. 2014;43(5):732-7.
36
Thomas AW, Davies NA, Moir H, Watkeys L, Ruffino JS, Isa SA, et al. Exercise-associated generation of PPARγ ligands activates PPARγ signaling events and upregulates genes related to lipid metabolism. J Appl Physiol. 2011;112(5): 806-15.
37
Li Y, Ge C, Long JP, Begun DL, Rodriguez JA, Goldstein SA, et al., Biomechanical stimulation of osteoblast gene expression requires phosphorylation of the RUNX2 transcription factor. J Bone Miner Res. 2012;27(6):1263-74.
38
Ziros PG Basdra EK, Papavassiliou AG. Runx2: of bone and stretch. Int. J Biochem Cell Biol. 2008;40(9):1659-63.
39
Singulani MP, Stringhetta-Garcia CT, Santos LF, Morais SRL, Louzada MJQ, Oliveira SHP, et al. Effects of strength training on osteogenic differentiation and bone strength in aging female Wistar rats. Sci Rep-UK. 2017;7(1):42878-9.
40
Turner CH, Takano Y, Owan I. Aging changes mechanical loading thresholds for bone formation in rats. J Bone Miner Res. 1995;10(10):1544-9.
41
Aido MIFd. The influence of age and mechanical loading on bone structure and material properties. TU Berlin: IBMS BoneKE; 2015: 84-8
42
Going SB, Farr JN. Exercise and bone macro-architecture: is childhood a window of opportunity for osteoporosis prevention? Int J Body Compos Res. 2010;8(1):1-9.
43
Razi H, Birkhold AI, Weinkamer R, Duda GN, Willie BM, Checa S. Aging leads to a dysregulation in mechanically driven bone formation and resorption. J Bone Miner Res. 2015;30(10):1864-73.
44
Heinonen A, Oja P, Kannus P, Sievanen H, Haapasalo H, Mänttäri A, et al. Bone mineral density in female athletes representing sports with different loading characteristics of the skeleton. Bone. 1995;17(3):197-203.
45
Wheater G, Elshahaly M, Tuck SP, Datta HK, van Laar JM. The clinical utility of bone marker measurements in osteoporosis. J Transl Med. 2013;11(1):201-14.
46
Shimamura C, Iwamoto J, Takeda T, Ichimura S, Abe H, Toyama Y. Effect of decreased physical activity on bone mass in exercise-trained young rats. J Orthop Sci. 2002;7(3):358-63.
47
Sinaki M, Wahner HW, Bergstralh EJ, Hodgson SF, Offord KP, Squires RW, et al. Three-year controlled, randomized trial of the effect of dose-specified loading and strengthening exercises on bone mineral density of spine and femur in nonathletic, physically active women. Bone. 1996;19(3):233-44.
48
Turner C. Functional determinants of bone structure: beyond Wolff's law of bone transformation. Elsevier. 1992;13(6): 403-9.
49
Forwood MR, Burr DB. Physical activity and bone mass: Exercises in futility. Bone Miner. 1993;21(2):89-112.
50
Kotha SP, Hsieh YF, Strigel RM, Muller R, Silva MJ. Experimental and finite element analysis of the rat ulnar loading model-correlations between strain and bone formation following fatigue loading. J Biomech. 2004;37(4):541-8.
51
Uthgenannt BA, Silva MJ. Use of the rat forelimb compression model to create discrete levels of bone damage in vivo. J Biomech. 2007;40(2):317-24.
52
Luu YK, Capilla E, Rosen CJ, Gilsanz V, Pessin JE, Judex S, et al. Mechanical stimulation of mesenchymal stem cell proliferation and differentiation promotes osteogenesis while preventing dietary‐induced obesity. J Bone Miner Res. 2009;24(1):50-61.
53
David V, Martin A, Lafage-Proust MH, Malaval L, Peyroche S, Jones DB, et al. Mechanical loading down-regulates peroxisome proliferator-activated receptor γ in bone marrow stromal cells and favors osteoblastogenesis at the expense of adipogenesis. Endocrinology. 2007;148(5):2553-62.
54
Sen B, Xie Z, Case N, Ma M, Rubin C, Rubin J. Mechanical strain inhibits adipogenesis in mesenchymal stem cells by stimulating a durable beta-catenin signal. Endocrinology. 2008;149(12):6065-75.
55
Tanabe Y, Koga M, Saito M, Matsunaga Y, Nakayama K. Inhibition of adipocyte differentiation by mechanical stretching through ERK-mediated downregulation of PPARgamma2. J Cell Sci. 2004;117(16):3605–14
56
Marie P, Kaabeche K. PPAR gamma activity and control of bone mass in skeletal unloading. Ppar Res. 2006; 2006(1): 1-6.
57
ORIGINAL_ARTICLE
تأثیر تمرین تناوبی پرشدت در موش صحرایی مادة چاق قبل از بارداری بر زمان خستگی، متیلاسیون ژنهای PGC-1α و سارکولیپین در عضلة دوقلوی زادههای موش
چاقی و نیاز به کنترل انرژی، درسراسر جهان بهعنوان یکی از بزرگترین عوامل در بروز بیماریهای مزمن محسوب میشوند و بهدلیل انتقال مضرات آن از مادر به فرزند بسیار اهمیت دارند؛ بنابراین، هدف از انجام پژوهش حاضر، بررسی تأثیر تمرین تناوبی پرشدت در موش صحرایی مادة چاق قبل از بارداری بر زمان خستگی، متیلاسیون ژنهایPeroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) و سارکولیپین در عضلة دوقلوزادههای موش بود؛ برایناساس، 40 موش صحرایی سهماهة مادة نژاد ویستار خریداری شدند و به چهار گروه مساوی تغذیة پرچرب، (گرم 22 ± 250) کنترل (گرم 30 ± 170)، تمرین + تغذیة پرچرب (گرم 30 ± 240) و تمرین (گرم 33 ± 190) تقسیم شدند. بعد از انجام شش هفته پروتکل تمرین تناوبی شدید، موشهای ماده باردار شدند و پس از تولد نوزادان، آنها به دو گروه تقسیم شدند. گروه اول برای بررسی متیلاسیون PGC-1α و سارکولیپین، به روشواکنش زنجیرهای پلیمراز اختصاصیتشریح شدند و گروه دوم بعد از دوماه، پروتکل شنا برای سنجش خستگی را انجام دادند. نتایج آزمون آنوای یکطرفه نشان داد که کاهش معناداری (P = 0.001) در مقادیر متیلاسیون ژن PGC-1α و افزایش معناداری (P = 0.001) در آزمون سنجش مقاومت به خستگی، بین گروه تمرین با گروههای دیگر و گروه تمرین + تغذیة پرچرب با گروه تغذیة پرچرب وجود داشتند؛ بنابراین، انجام تمرینهای تناوبی شدید قبل از بارداری بر متیلاسیون ژن PGC-1αو عملکرد ورزشی نوزادان موش صحرایی در مقاومت به خستگی نقش دارد. همچنین، تمرین تناوبی شدید میتواند تأثیر ناشی از مصرف غذای پرچرب را کاهش دهد.
https://spj.ssrc.ac.ir/article_1421_a55d2001fc7e7a085029b4f0917dc8da.pdf
2019-07-23
79
94
10.22089/spj.2018.6188.1799
سارکولیپین
اپیژنتیک
تمرین تناوبی پرشدت
PGC-1α
سمانه
کنشلو
s.koneshlou@yahoo.com
1
دانشجوی دکتری فیزیولوژی ورزشی، دانشگاه شهید بهشتی، تهران، ایران
AUTHOR
مریم
نورشاهی
m-nourshahi@sbu.ac.ir
2
دانشیار فیزیولوژی ورزشی، دانشگاه شهید بهشتی، تهران، ایران
LEAD_AUTHOR
مهدی
هدایتی
hedayati47@gmail.com
3
دانشیار بیوشیمی، دانشگاه علوم پزشکی شهید بهشتی
AUTHOR
رعنا
فیاض میلانی
milani.sbu@hotmail.com
4
استادیار فیزیولوژی ورزشی دانشگاه شهید بهشتی، تهران، ایران
AUTHOR
Adair LS, Fall CH, Osmond C, Stein AD, Martorell R, Ramirez-Zea M, et al. Associations of linear growth and relative weight gain during early life with adult health and human capital in countries of low and middle income: findings from five birth cohort studies. Lancet. 2013;382(9891):525-34.
1
Cedergren M, Brynhildsen J, Josefsson A, Sydsjo A, Sydsjo G. Hyperemesis gravidarum that requires hospitalization and the use of antiemetic drugs in relation to maternal body composition. Am J Obstet Gynecol. 2008 Apr;198(4):412.e1-5. doi: 10.1016/j.ajog.2007.09.029. Epub 2008 Jan 25
2
Mesman I, Roseboom TJ, Bonsel GJ, Gemke RJ, van der Wal MF, Vrijkotte TG. Maternal pre-pregnancy body mass index explains infant's weight and BMI at 14 months: results from a multi-ethnic birth cohort study. Arch Dis Child. 2009 Aug;94(8):587-95. doi: 10.1136/adc.2008.137737. Epub 2009 Mar 29.
3
Whitaker RC. Predicting preschooler obesity at birth: the role of maternal obesity in early pregnancy. Pediatrics. 2004;114(1):29-36.
4
Tequeanes ALL, Gigante DP, Assunção MCF, Chica DAG, Horta BL. Maternal anthropometry is associated with the body mass index and waist: height ratio of offspring at 23 years of age. J Nutr. 2009;139(4):750-4.
5
Laker RC, Lillard TS, Okutsu M, Zhang M, Hoehn KL, Connelly JJ, et al. Exercise prevents maternal high-fat diet-induced hypermethylation of the Pgc-1alpha gene and age-dependent metabolic dysfunction in the offspring. Diabetes. 2014;63(5):1605-11.
6
Barres R, Yan J, Egan B, Treebak JT, Rasmussen M, Fritz T, et al. Acute exercise remodels promoter methylation in human skeletal muscle. Cell Metab. 2012;15(3):405-11.
7
Scarpulla RC. Metabolic control of mitochondrial biogenesis through the PGC-1 family regulatory network. Biochim Biophys Acta. 2011;1813(7):1269-78.
8
Lin J, Wu H, Tarr PT, Zhang C-Y, Wu Z, Boss O, et al. Transcriptional co-activator PGC-1α drives the formation of slow-twitch muscle fibres. Nature. 2002;418(6899):797.
9
Leone TC, Lehman JJ, Finck BN, Schaeffer PJ, Wende AR, Boudina S, et al. PGC-1alpha deficiency causes multi-system energy metabolic derangements: muscle dysfunction, abnormal weight control and hepatic steatosis. PLoS biology. 2005;3(4): 101.
10
Odermatt A, Becker S, Khanna VK, Kurzydlowski K, Leisner E, Pette D, et al. Sarcolipin regulates the activity of SERCA1, the fast-twitch skeletal muscle sarcoplasmic reticulum Ca2+-ATPase. J Biol Chem. 1998;273(20):12360-9.
11
Odermatt A, Taschner PE, Scherer SW, Beatty B, Khanna VK, Cornblath DR, et al. Characterization of the gene encoding human sarcolipin (SLN), a proteolipid associated with SERCA1: absence of structural mutations in five patients with Brody disease. Genomics. 1997;45(3):541-53.
12
Maurya SK, Bal NC, Sopariwala DH, Pant M, Rowland LA, Shaikh SA, et al. Sarcolipin Is a Key Determinant of the Basal Metabolic Rate, and Its Overexpression Enhances Energy Expenditure and Resistance against Diet-induced Obesity. J Biol Chem. 2015;290(17):10840-9.
13
Smith WS, Broadbridge R, East JM, Lee AG. Sarcolipin uncouples hydrolysis of ATP from accumulation of Ca2+ by the Ca2+-ATPase of skeletal-muscle sarcoplasmic reticulum. Biochem J. 2002;361(Pt 2):277-86.
14
Sopariwala DH, Pant M, Shaikh SA, Goonasekera SA, Molkentin JD, Weisleder N, et al. Sarcolipin overexpression improves muscle energetics and reduces fatigue. J Appl Physiol. 2015;118(8):1050-8.
15
Bruton JD, Aydin J, Yamada T, Shabalina IG, Ivarsson N, Zhang SJ, et al. Increased fatigue resistance linked to Ca2+‐stimulated mitochondrial biogenesis in muscle fibres of cold‐acclimated mice. J Physiol. 2010;588(21):4275-88.
16
Calvo JA, Daniels TG, Wang X, Paul A, Lin J, Spiegelman BM, et al. Muscle-specific expression of PPARγ coactivator-1α improves exercise performance and increases peak oxygen uptake. J Appl Physiol. 2008;104(5):1304-12.
17
Castillo H, Santos IS, Matijasevich A. Relationship between maternal pre-pregnancy body mass index, gestational weight gain and childhood fatness at 6-7 years by air displacement plethysmography. Matern Child Nutr. 2015;11(4):606-17.
18
King HD. The relation of age to fertility in the rat. The Anatomical Record. 1916;11(5):269-87.
19
Hao Y-R, Tang F-J, Zhang X, Wang H. Suppression of NF-κB activation by PDLIM2 restrains hepatic lipogenesis and inflammation in high fat diet induced mice. Biochem Biophys Res Commun. 2018;503(2):564-71.
20
Zou T, Wang B, Yang Q, de Avila JM, Zhu M-J, You J, et al. Raspberry promotes brown and beige adipocyte development in mice fed high-fat diet through activation of AMP-activated protein kinase (AMPK) α1. J Nutr Biochem. 2018;55:157-64.
21
Afzalpour ME, Chadorneshin HT, Foadoddini M, Eivari HA. Comparing interval and continuous exercise training regimens on neurotrophic factors in rat brain. Physiol Behav. 2015;147:78-83.
22
Høydal MA, Wisløff U, Kemi OJ, Ellingsen Ø. Running speed and maximal oxygen uptake in rats and mice: practical implications for exercise training. Eur J Cardiovasc Prev Rehabil. 2007;14(6):753-60.
23
Barres R, Osler ME, Yan J, Rune A, Fritz T, Caidahl K, et al. Non-CpG methylation of the PGC-1alpha promoter through DNMT3B controls mitochondrial density. Cell Metab. 2009;10(3):189-98.
24
Lima F, Stamm D, Della Pace I, Ribeiro L, Rambo L, Bresciani G, et al. Ibuprofen intake increases exercise time to exhaustion: A possible role for preventing exercise‐induced fatigue. Scand J Med Sci Sports. 2016;26(10):1160-70.
25
Laker RC, Lillard TS, Okutsu M, Zhang M, Hoehn KL, Connelly JJ, et al. Exercise prevents maternal high-fat diet-induced hypermethylation of the Pgc-1α gene and age-dependent metabolic dysfunction in the offspring. Diabetes. 2014:DB_131614.
26
Sheldon RD, Blaize AN, Fletcher JA, Pearson KJ, Donkin SS, Newcomer SC, et al. Gestational exercise protects adult male offspring from high-fat diet-induced hepatic steatosis. J Hepatol. 2016;64(1):171-8.
27
Stanford KI, Lee M-Y, Getchell KM, So K, Hirshman MF, Goodyear LJ. Exercise before and during pregnancy prevents the deleterious effects of maternal high-fat feeding on metabolic health of male offspring. Diabetes. 2014:DB_131848.
28
Vega CC, Reyes-Castro LA, Bautista CJ, Larrea F, Nathanielsz PW, Zambrano E. Exercise in obese female rats has beneficial effects on maternal and male and female offspring metabolism. Int J Obes (Lond). 2015;39(4):712.
29
Gibala M. Molecular responses to high-intensity interval exercise. Applied Physiology, Nutrition, and Metabolism. 2009;34(3):428-32.
30
Nitert MD, Dayeh T, Volkov P, Elgzyri T, Hall E, Nilsson E, et al. Impact of an exercise intervention on DNA methylation in skeletal muscle from first-degree relatives of patients with type 2 diabetes. Diabetes. 2012;61(12):3322-32.
31
Borengasser SJ, Lau F, Kang P, Blackburn ML, Ronis MJ, Badger TM, et al. Maternal obesity during gestation impairs fatty acid oxidation and mitochondrial SIRT3 expression in rat offspring at weaning. PloS one. 2011;6(8):24068.
32
Hastie R, Lappas M. The effect of pre-existing maternal obesity and diabetes on placental mitochondrial content and electron transport chain activity. Placenta. 2014;35(9):673-83.
33
De Fante T, Simino LA, Reginato A, Payolla TB, Vitoréli DCG, de Souza M, et al. Diet-induced maternal obesity alters insulin signalling in male mice offspring rechallenged with a high-fat diet in adulthood. PloS one. 2016;11(8):0160184.
34
De Araujo GG, Papoti M, de Barros Manchado F, de Mello MAR, Gobatto CA. Protocols for hyperlactatemia induction in the lactate minimum test adapted to swimming rats. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology Comp Biochem Physiol A Mol Integr Physiol. 2007;148(4):888-92.
35
Boutcher SH. High-intensity intermittent exercise and fat loss. J Obes. 2011;2011:868305.161-10
36
Little JP, Safdar A, Cermak N, Tarnopolsky MA, Gibala MJ. Acute endurance exercise increases the nuclear abundance of PGC-1alpha in trained human skeletal muscle. Am J Physiol Regul Integr Comp Physiol. 2010;298(4):912-7.
37
ORIGINAL_ARTICLE
هنجاریابی آزمون آمادگی جسمانی استاندارد کانادایی (CSTF) در دانشآموزان با کمتوان ذهنی مقاطع متوسطۀ اول و متوسطة دوم استان همدان
هدف این پژوهش، نورمسنجی آمادگی جسمانی دانشآموزان کمتوان ذهنی توسط آزمون آمادگی جسمانی استاندارد کانادایی (CSTF) بود؛ برایناساس، 314 دانشآموز (115 دختر و 199 پسر) کمتوان ذهنی آموزشپذیر (دامنۀ بهرۀ هوشی: 70-50، سن: 55/2 ± 53/18 سال)، ازطریق مدارس استثنایی استان همدان انتخاب شدند. نخست، قد، وزن، و نمایۀ تودۀ بدنی دانشآموزان سنجیده شدند. سپس، آزمونهای میدانی انعطافپذیری، استقامت و قدرت ماهیچهای و اکسیژن مصرفی بیشینه (VO2max)، توسط مجموعة آزمون CSTF برآورد شدند. آمار توصیفی، آزمون آنوای یکسویه و صدکهای 20، 40، 60 و 80، برای آنالیز دادهها و تدوین هنجارهای آن استفاده شدند.نتایج نشان داد که تفاوت معناداری بین دختران و پسران در هریک از متغیرهای استقامت عضلانی، قدرت ماهیچهای و اکسیژن مصرفی بیشینه وجود داشت (0.05 >P)؛ اما انعطافپذیری تفاوت معناداری را نشان نداد (0.05P >). میانگین و انحراف استاندارد آزمون انعطافپذیری 89/9 ± 85/19 (سانتیمتر)، شنای سوئدی 48/8 ± 89/11 (عدد)، درازونشست 54/9 ± 26/19 (عدد در دقیقه)، قدرت پنجه 64/5 ± 74/14 (کیلوگرم) و ظرفیت هوازی 97/5 ± 51/60 (میلیلیتر بر کیلوگرم در دقیقه) بود. دادههای پژوهش حاضر میتوانند برای نمرهدهی و درجهبندی دانشآموزان دختر و پسر کمتوان ذهنی استان همدان بهکار روند.
https://spj.ssrc.ac.ir/article_1606_8691bf6bfcd10c19991ba45b67a8bc88.pdf
2019-07-23
95
112
10.22089/spj.2019.6414.1816
آزمون کانادایی
نورمسنجی
کمتوان ذهنی
آمادگی بدنی
قدرت
استقامت
انعطافپذیری
حجت اله
سیاوشی
seiavoshy@gmail.com
1
دانشجوی دکتری فیزیولوژی ورزشی، پژوهشگاه تربیت بدنی و علوم ورزشی، تهران، ایران
LEAD_AUTHOR
حمید
آقا علی نژاد
halinejad@modares.ac.ir
2
دانشیار فیزیولوژی ورزشی، دانشگاه تربیت مدرس، تهران، ایران
AUTHOR
علی
کاشی
ssrc.kashi@gmail.com
3
استادیار رفتار حرکتی، پژوهشگاه تربیتبدنی و علوم ورزشی، تهران، ایران
AUTHOR
محمد علی
سمواتی شریف
m-samavati@basu.ac.ir
4
دانشیار فیزیولوژی ورزشی، دانشگاه بوعلی سینا، همدان، ایران
AUTHOR
معصومه
هلالی زاده
m.helalizadeh@ssrc.ac.ir
5
استادیار فیزیولوژی ورزشی، پژوهشگاه تربیتبدنی و علوم ورزشی، تهران، ایران
AUTHOR
Samavati Sharif M, Afshari A, Siavoshy H, Keshvary M. The effect of two exercises training on some of immune system markers in adolescent athletes. Journal of Practical Studies of Biosciences in Sport (JPSBS). 2016;4(8):55-65. (Persian(
1
Afshari A, Samavati Sharif MA, Siavoshy H. Comparison of Speed and Strength Training to Maintain Hematological Factors and Vo2max of Male Athletes 13 to 15 Years. JsportPec. 2015;12(23):53-64. (Persian(
2
Kajbaf MB, Mansuri M, Ejehei J, Dadsetan P. Investigating of diagnosis of "intellectual disability" based on Piaget's tests and Lambert's adaptive behavioral scale. J Psychol. 2000;3(4):341-57.
3
Siavoshy H, Seddighi A. The Effects of a Balance Exercise Program for Enhancement of Gait Function on Temporal and Spatial Gait Parameters in Young People with Intellectual Disabilities. Exceptional Education. 2016;1(138):68-73. (Persian(
4
Siavoshy H. Effects of Two Type Exercise Training Programs on Body Composition of Adolescence with Down Syndrome. Exceptional Education. 2015;3(131):65-72. (Persian(
5
Siyavoshi H. Progressive Tolerance Exercises for Young adults Suffering from down syndrome: A Clinical Experiment. Exceptional Education. 2013;5(118):68-71. (Persian(
6
Siavoshy H, Bolurian F. The effects of 12 weeks of playing boccia on the social development of children with cerebral palsy and intellectual disability. Journal of Exceptional Children. 2016;15(4):45-51. (Persian(
7
Siavoshy H. Effects of Resistance Training on Salivary Hormone Profile and Immunoglobulin A in Adults with Down Syndrome. Exceptional Education. 2016;9(137):60-4. (Persian(
8
Sedighi A, Anbarian M, Siavoshy H, editors. Effect of 8 Weeks Resistance Training on Muscle Strength in Adolescents with Down Syndrome. The First International Conference on Sport Science; 2015; Tehran, Iran.
9
Abdullah NM, Hamid NA, Tumijan W, Parnabas V, Rahim MRA, Ismail S, et al., editors. The Differences Between Students with Intellectual Disabilities and Normal Students on the Physical Fitness Level. Proceedings of the International Colloquium on Sports Science, Exercise, Engineering and Technology 2014 (ICoSSEET 2014); 2014; Singapore: Springer.
10
Graham A, Reid G. Physical fitness of adults with an intellectual disability: A 13-year follow-up study. Res Q Exerc Sport. 2000;71(2):152-61.
11
Karinharju K. Physical Fitness and its testing in adults with intellectual disability. Department of sport sciences, University of Jyvaskyla, Finland [Jyvaskyla]: Master’s Thesis. Available From: URL: https://jyx. jyu. fi/dspace/bitstream/handle/123456789/9 670/URN_NBN_fi_jyu-2005401. pdf; 2005. p. 7-77.
12
Vaupshas R. The exercise intensity of mentally retarded adults as a function of an aerobic fitness program: McGill University Montreal, Quebec; 1989. p.14-105.
13
Rarick GL. Motor performance of mentally retarded children. Physical activity: Human growth and development. 1973;1(1):225-56.
14
van Schijndel‐Speet M, Evenhuis H, van Wijck R, van Montfort K, Echteld M. A structured physical activity and fitness programme for older adults with intellectual disabilities: results of a cluster‐randomised clinical trial. J Intellect Disabil Res. 2017;61(1):16-29.
15
Lahtinen U, Rintala P, Malin A. Physical performance of individuals with intellectual disability: A 30-year follow-up. Adapted Physical Activity Quarterly. 2007;24(2):125-43.
16
Sherrill C. Adapted physical activity, recreation and sport: Crossdisciplinary and lifespan. 6 ed. Boston: MA: McGraw-Hill; 2004. p. 10-706.
17
Hematfar A, Sharif MAS, Valizadeh Y, Siavoshy H, Keihanshokouh J. Effect of a Six-week Combined Aerobic and Resistance Exercise Training on Some Liver Function Parameters in Middle-aged Men with Non-alcoholic Fatty Liver Disease. Scientific Journal of Hamadan University of Medical Sciences and Health Services. 2017;24(38): 206-14. (Persian(
18
Lotan M, Yalon-Chamovitz S, Weiss PLT. Virtual reality as means to improve physical fitness of individuals at a severe level of intellectual and developmental disability. Res Dev Disabil. 2010;31(4):869-74.
19
Frey G, Chow B. Relationship between BMI, physical fitness, and motor skills in youth with mild intellectual disabilities. Int J Obes. 2006;30(5):861–7.
20
Boer P, Moss S. Test–retest reliability and minimal detectable change scores of twelve functional fitness tests in adults with Down syndrome. Res Dev Disabil. 2016;48:176-85.
21
Hilgenkamp TI, van Wijck R, Evenhuis HM. Feasibility and reliability of physical fitness tests in older adults with intellectual disability: a pilot study. Journal of Intellectual and Developmental Disability. 2012;37(2):158-62.
22
Hilgenkamp TI, van Wijck R, Evenhuis HM. Feasibility of eight physical fitness tests in 1,050 older adults with intellectual disability: Results of the healthy ageing with intellectual disabilities study. Intellectual and developmental disabilities. 2013;51(1):33-47.
23
Mohammadi F, Rajabi R, Alizadeh MH, VaezMousavi M. Relationship between IQ Levels and Physical Fitness Factors in Students with Intellectual Disability. Sport Psychology Studies. 2015;3(10):69-74. (Persian(
24
Monks C. The impact of the Canadian Standardized Test of Fitness and of health counselling on health attitudes and behaviour: Brock University, Ontario [Catharines]; 1996.
25
Fernhall B. Limitations to physical work capacity in individuals with mental retardation. Clin Exerc Physiol. 2001;3:176-85.
26
Wouters M, Evenhuis HM, Hilgenkamp TI. Systematic review of field-based physical fitness tests for children and adolescents with intellectual disabilities. Res Dev Disabil. 2017;61:77-94.
27
Samavati Sharif MA, Bagheri S, Siavoshy H. Comparison Anthropometrical and Physiological Profile Between National Handball Women's Players and Hamadan's Handball Women's Players. Research on Biosciences and Physical Actiuity. 2015;2(3):47-54. (Persian(
28
Government of Canada. Canadian Standardized Test of Fitness (CSTF) Operations Manual. 3 ed. Ottawa, Canada: Fitness and Amateur Sport Canada. FAS 7378; 1986.
29
Schalock RL, Borthwick-Duffy SA, Bradley VJ, Buntinx WH, Coulter DL, Craig EM, et al. Intellectual disability: Definition, classification, and systems of supports. 11, editor. American Association on Intellectual and Developmental Disabilities: 444 North Capitol Street NW Suite 846, Washington, DC 20001; 2010. p. 10-259.
30
Reid G, Montgomery DL, Seidl C. Performance of mentally retarded adults on the Canadian Standardized Test of Fitness. Canadian journal of public health= Revue canadienne de sante publique. 1985;76(3):187-90.
31
Weller IM, Thomas SG, Gledhill N, Paterson D, Quinney A. A study to validate the modified Canadian Aerobic Fitness Test. Can J Appl Physiol. 1995;20(2):211-21.
32
Garcia AW, Zakrajsek JS. Evaluation of the Canadian Aerobic Fitness Test with 10-to 15-year-old children. Pediatr Exerc Sci. 2000;12(3):300-11.
33
Shephard RJ, Bouchard C. A new approach to the interpretation of Canadian Home Fitness Test scores. Can J Appl Physiol. 1993;18(3):304-16.
34
Pitetti KH, Fernhall B, Stubbs N, Stadler Jr LV. A step test for evaluating the aerobic fitness of children and adolescents with mental retardation. Pediatr Exerc Sci. 1997;9(2):127-35.
35
Jetté M, Campbell J, Mongeon J, Routhier R. The Canadian Home Fitness Test as a predictor of aerobic capacity. Can Med Assoc J. 1976;114(8):680–2.
36
Montgomery D, Reid G, Seidl C. The effects of two physical fitness programs designed for mentally retarded adults. Canadian journal of sport sciences= Journal canadien des sciences du sport. 1988;13(1):73-8.
37
Montgomery D, Reid G, Koziris L. Reliability and validity of three fitness tests for adults with mental handicaps. Can J Sport Sci. 1992;17(4):309-15.
38
Farahani A, Seraj S. Nationai Index of Motor-Skill Fitness in comprehensive Volunteer of Payame Noor University Case Study:Comprehensive Undergraduate Female Volunteer. Scientific Journal Management System. 2014;3(6):87-94.
39
Samavati Sharif MA, Rajabi A, Siavoshi H. The Effects of 6-Weeks Aerobic Exercise Training on Blood Hematological Factors in Adolescence Girls. The Iranian Journal of Obstetrics, Gynecology and Infertility. 2016; 37(19): 8-15. (Persian(
40
Siavoshy H, Mohammadi M, Hojjati SS, editors. Investigating the relationship between aerobic capacity and muscular strength in intellectual disability children. The First Conference on Applied Research in Sport Science; 2017; Hamadan, Iran.
41
Short FX, Winnick JP. Test items and standards related to muscle strength and Endurance on the Brockport physical fitness test. Adapted Physical Activity Quarterly. 2005;22(4):371-400.
42
Rahmani P, Shahrokhi H, Dansehmandi H. Comparative study of physical fitness factors in intellectual disability with and without Down syndrome. Studies in Sport Medicine. 2012;4(11):81-94. (Persian(
43
Place M, Dickinson K, Reynolds J. Do we need norms of fitness for children with autistic spectrum condition? British Journal of Special Education. 2015;42(2):199-216.
44
ORIGINAL_ARTICLE
اثر دو الگوی تمرین با نسبت روزهای استراحت به تمرین متفاوت و مصرف مکمل عسل طبیعی بر غلظت اینترلوکین-6 سرم و بیان آن در بافت هیپوکمپ موشهای صحرایی نر نابالغ
هدف از انجام پژوهش حاضر، بررسی تأثیر دو الگوی تمرین با نسبت روزهای استراحت به تمرین متفاوت همراه با مصرف عسل طبیعی، بر میزانIL-6 سرم و بیان آن در بافت هیپوکمپ بود. 36 موش صحرایی نر نابالغ در شش گروه کنترل، عسل (10 درصد در آب آشامیدنی)، تمرین تناوبی شدید چهارروزه، تمرین تناوبی شدید چهارروزه + عسل، تمرین تناوبی شدید هفتروزه و تمرین تناوبی شدید هفتروزه + عسل قرار گرفتند. دورههای تمرین چهارروزه (سه روز تمرین و یک روز استراحت) و هفتروزه (شش روز تمرین، یک روز استراحت) تناوبی شدید (16-10 متر در دقیقه در هفتة اول و 40-36 متر در دقیقه در هفتة آخر) بهمدت یک ماه انجام شد. غلظت IL-6 سرم در گروه تمرین هفتروزه نسبت به گروه کنترل بالاتر بود (P = 0.001). سطوح IL-6 سرم در گروه عسل- تمرین هفتروزه پایینتر از گروه تمرین هفتروزه بدون مکمل (P = 0.018) بود. بین گروه چهارروزه و گروه کنترل تفاوت معناداری وجود نداشت (P = 0.946). هر دو نوع تمرینهای هفتروزه (P = 0.007) و چهارروزه (P = 0.005) موجب بالاتررفتن معنادار بیان ژن IL-6 هیپوکمپ شدند؛ اما مصرف مکمل عسل در گروه هفتروزه (P = 0.583) و چهارروزه (P = 0.983) موجب تعدیل بیان ژن IL-6 هیپوکمپ نشد؛ بنابراین، تمرینهای تناوبی شدید بیان ژن IL-6 را در هیپوکمپ افزایش میدهند؛ اما دورههای تمرینی طولانیتر موجب افزایش سیستمیک سایتوکاینهای التهابی میشوند. مصرف عسل اثرهای مرکزی تمرینهای تناوبی شدید را تعدیل نمیکند؛ بااینحال، میتواند التهاب سیستمیک را کاهش دهد. همچنین، اثرهای ضدالتهابی عسل در شرایط غیرتمرینی و غیرالتهابی، مشاهده نمیشوند.
https://spj.ssrc.ac.ir/article_1670_1c87777a7e3ad7a98329898d0d7f0215.pdf
2019-07-23
113
132
10.22089/spj.2019.3748.1505
تمرین تناوبی شدید
عسل
اینترلوکین-6
هیپوکمپ
احمد
رحمانی
a_rahmani@znu.ac.ir
1
استادیار رفتار حرکتی، دانشگاه زنجان، زنجان، ایران
LEAD_AUTHOR
علی
گرزی
ali_gorzi@znu.ac.ir
2
دانشیار فیزیولوژی ورزشی، دانشگاه زنجان، زنجان، ایران
AUTHOR
زهرا
محمدی
zahramohamadi979@gmail.com
3
کارشناسیارشد فیزیولوژی ورزشی کاربردی، دانشگاه زنجان، زنجان، ایران
AUTHOR
Andersen SL. Trajectories of brain development: point of vulnerability or window of opportunity? Neurosci Biobehav Rev. 2003;27(1-2):3-18.
1
Uysal N, Tugyan K, Kayatekin BM, Acikgoz O, Bagriyanik HA, Gonenc S, et al. The effects of regular aerobic exercise in adolescent period on hippocampal neuron density, apoptosis and spatial memory. Neurosci Lett. 2005;383(3):241-45.
2
Kim T-W, Ji E-S, Kim T-W, Lee S-W, Lee C-Y, Lee S-J. Postnatal treadmill exercise attenuates prenatal stress-induced apoptosis through enhancing serotonin expression in aged-offspring rats. J Exerc Rehabil. 2015;11(1):12-9.
3
Gibala MJ, McGee SL. Metabolic adaptations to short-term high-intensity interval training: a little pain for a lot of gain? Exerc Sport Sci Rev. 2008;36(2):58-63.
4
De Almeida AA, da Silva SG, Fernandes J, Peixinho-Pena LF, Scorza FA, Cavalheiro EA, et al. Differential effects of exercise intensities in hippocampal BDNF, inflammatory cytokines and cell proliferation in rats during the postnatal brain development. Neurosci Lett. 2013;553:1-6.
5
Secher NH, Seifert T, Van Lieshout JJ. Cerebral blood flow and metabolism during exercise: implications for fatigue. J Appl Physiol. 2008;104:306-14.
6
Chennaoui M, Drogou C, Gomez-Merino D. Effects of physical training on IL-1β, IL-6 and IL-1ra concentrations in various brain areas of the rat. Eur Cytokine Netw. 2008;19(1):8-14.
7
Colbert L, Davis J, Essig D, Ghaffar A, Mayer E-P. Tissue expression and plasma concentrations of TNFα, IL-1β, and IL-6 following treadmill exercise in mice. Int J Sports Med. 2001;22(04):261-7.
8
Zhang J-M, An J. Cytokines, inflammation and pain. Int Anesthesiol Clin. 2007;45(2):27-37.
9
Smith LL. Overtraining, excessive exercise, and altered immunity. Sports Med. 2003;33(5):347-64.
10
Wang W-Y, Tan M-S, Yu J-T, Tan L. Role of pro-inflammatory cytokines released from microglia in Alzheimer's disease. Ann Transl Med. 2015;3(10):136-51.
11
Suzuki K, Nakaji S, Yamada M, Totsuka M, Sato K, Sugawara K. Systemic inflammatory response to exhaustive exercise. Cytokine kinetics. Exerc Immunol Rev. 2002;8:6-48.
12
European journal of pharmacologyInternational journal of biological sciencesBoots AW, Haenen GR, Bast A. Health effects of quercetin: from antioxidant to nutraceutical. Eur J Pharmacol. 2008;585(2-3):325-37.
13
Erta M, Quintana A, Hidalgo J. Interleukin-6, a major cytokine in the central nervous system. Int J Biol Sci. 2012;8(9):1254-66.
14
Lou S-j, Liu J-y, Chang H, Chen P-j. Hippocampal neurogenesis and gene expression depend on exercise intensity in juvenile rats. Brain Res. 2008;1210:48-55.
15
De Almeida AA, Gomes da Silva S, Fernandes J, Peixinho-Pena LF, Scorza FA, Cavalheiro EA, et al. Differential effects of exercise intensities in hippocampal BDNF, inflammatory cytokines and cell proliferation in rats during the postnatal brain development. Neurosci Lett. 2013;553:1-6.
16
Lezi E, Burns JM, Swerdlow RH. Effect of high-intensity exercise on aged mouse brain mitochondria, neurogenesis, and inflammation. Neurobiol Aging. 2014;35(11):2574-83.
17
De Souza DC, Matos VAF, Dos Santos VOA, Medeiros IF, Marinho CSR, Nascimento PRP, et al. Effects of High-Intensity Interval and Moderate-Intensity Continuous Exercise on Inflammatory, Leptin, IgA, and Lipid Peroxidation Responses in Obese Males. Front Physiol. 2018;9:567-76.
18
Heavens KR, Szivak TK, Hooper DR, Dunn-Lewis C, Comstock BA, Flanagan SD, et al. The effects of high intensity short rest resistance exercise on muscle damage markers in men and women. J Strength Cond Res. 2014;28(4):1041-9.
19
Navalta JW, Tibana RA, Fedor EA, Vieira A, Prestes J. Three consecutive days of interval runs to exhaustion affects lymphocyte subset apoptosis and migration. Biomed Res Int. 2014;2014:1-6
20
Ghazali WSW, Romli AC, Mohamed M. Effects of honey supplementation on inflammatory markers among chronic smokers: a randomized controlled trial. BMC Complement Altern Med. 2017;17(1):175-81.
21
Vallianou N, Gounari P, Skourtis A, Panagos J, Kazazis C. Honey and its anti-inflammatory, anti-bacterial and anti-oxidant properties. Gen Med (Los Angel). 2014;2(132):1-5.
22
Hadagali MD, Chua LS. The anti-inflammatory and wound healing properties of honey. Eur Food Res Technol. 2014;239(6):1003-14.
23
Salehian O, Rashidi M, Sedaghat M. Oral supplementation of natural honey and levels of inflammatory and anti-inflammatory plasma cytokines during 10-week of intensive tread-mill training in endurance-trained athletes. Biomed Res-India. 2014;25(4): 459-62.
24
Chepulis LM, Starkey NJ, Waas JR, Molan PC. The effects of long-term honey, sucrose or sugar-free diets on memory and anxiety in rats. Physiol Behav. 2009;97 (3-4):359-68.
25
Prakash A, Medhi B, Avti PK, Saikia UN, Pandhi P, Khanduja KL. Effect of different doses of Manuka honey in experimentally induced inflammatory bowel disease in rats. Phytother Res. 2008;22(11):1511-9.
26
Camelia Adly Abdel Malak KAKE, Mahmoud Mohamed Howas, Eslam Samy Elsherbiny. Protective Effect of Honey and Propolis against Carbon Tetrachloride (CCl4)-Induced Hepatotoxicity in Rats. Biochemistry. 2015;5(2):1-4.
27
Shepherd R, Gollnick P. Oxygen uptake of rats at different work intensities. Pflug Arch. 1976;362(3):219-22.
28
Mohebbi H, Garekani ET, Hedayati M, Fathi R. Effects of exercise training on high molecular weight adiponectin in healthy male rat. IJEM. 2009;11(3): 315-21. (in persian).
29
Bijeh N, Hejazi K, Delpasand A. Acute and Chronic Responses of Serum Leptin Hormone to Different Intensities of Exercise in Rats with Polycystic Ovarian Syndrome. Pathobio Res. 2015;18(1):95-106. (in persian).
30
Vincent HK, Powers SK, Stewart DJ, Demirel HA, Shanely RA, Naito H. Short-term exercise training improves diaphragm antioxidant capacity and endurance. Eur J Appl Physiol. 2000;81(1-2):67-74.
31
Freitas DA, Rocha-Vieira E, Soares BA, Nonato LF, Fonseca SR, Martins JB, et al. High intensity interval training modulates hippocampal oxidative stress, BDNF and inflammatory mediators in rats. Physiol Behav. 2018;184:6-11.
32
Zwetsloot KA, John CS, Lawrence MM, Battista RA, Shanely RA. High-intensity interval training induces a modest systemic inflammatory response in active, young men. J Inflamm Res. 2014;7:9-17.
33
Gorzi A, Ekradi S, Rahmani A. The Effect of 8 Weeks of Sprint Interval Training on Oxidative and Antioxidative Capacity of Heart, Liver and Skeletal Muscle in Male Wistar Rats. Sport Phys. 2018;10(37):123-38. (in persian).
34
Powers SK, Criswell D, Lawler J, Martin D, Lieu F, Ji LL, et al. Rigorous exercise training increases superoxide dismutase activity in ventricular myocardium. Am J Physiol. 1993;265(6):2094-8.
35
Dorak MT. Real-time PCR. Newcastle:Taylor & Francis Group; 2007:1-307
36
Sahhugi Z, Hasenan SM, Jubri Z. Protective effects of gelam honey against oxidative damage in young and aged rats. Oxid Med Cell Longev. 2014;2014:1-9.
37
Erejuwa OO, Sulaiman SA, Ab Wahab MS. Honey: a novel antioxidant. Molecules. 2012;17(4):4400-23.
38
Tavafzadeh SS, Ooi FK, Chen CK, Sulaiman SA. Changes in Bone Metabolism and Antioxidant Status with Combined Exercise and Honey Supplementation in Young Female Rats. J Exerc Sports Orthop. 2015;2(2):1-8.
39
Ma Y, Gao M, Sun H, Liu D. Interleukin-6 gene transfer reverses body weight gain and fatty liver in obese mice. Biochim Biophys Acta. 2015;1852(5):1001-11.
40
Smith LL. Cytokine hypothesis of overtraining: a physiological adaptation to excessive stress? Med Sci Sports Exerc. 2000;32(2):317-31.
41
Gorzi A, Tofighi A, Amiri B. The effects of curcumin supplementation on oxidative stress induced during strenuous endurance training on the kidney and lung tissue. SJKUMS. 2017;13(25):75-86. (in persian).
42
Freitas DA, Rocha-Vieira E, Soares BA, Nonato LF, Fonseca SR, Martins JB, et al. High intensity interval training modulates hippocampal oxidative stress, BDNF and inflammatory mediators in rats. Physiol Behav. 2018;184:6-11.
43
Sheikholeslami-vatani D, Eidi A, Boubani B, Abdi M. The effects of one vs. two exhaustive sessions exercise on Cortisol, TNF-α and Adenosine Deaminase enzyme in endurance runners. J Kermanshah Univ Med Sci. 2012;16(2):109-18. (in persian).
44
Cabral-Santos C, Castrillón CI, Miranda RA, Monteiro PA, Inoue DS, Campos EZ, et al. Inflammatory cytokines and BDNF response to high-intensity intermittent exercise: effect the exercise volume. Front Physiol. 2016;7:509-17.
45
Mogharnasi M, Gaeini A, Sheikholeslami Vatani D. Comparing the effects of two training methods of aerobic and anaerobic on some pre-inflammatory cytokines in adult male rats. IJEM. 2010;11(2):191-8. (in persian).
46
Pedersen BK. Exercise and cytokines. Immunol Cell Biol. 2000;78(5):532-5.
47
Nasuti C, Gabbianelli R, Falcioni G, Cantalamessa F. Antioxidative and gastroprotective activities of anti-inflammatory formulations derived from chestnut honey in rats. Nutr Res. 2006;26(3):130-7.
48
Chepulis LM. The effects of honey compared with sucrose and a sugar-free diet on neutrophil phagocytosis and lymphocyte numbers after long-term feeding in rats. JCIM. 2007;4(1):1-7.
49
Jalili L, Hajizadeh B, Mohammadzadeh H, Tartibian B. The effects of honey solution before maximal aerobic exercise on immune respones in active young men. J Urmia Univ Med Sci. 2010;21(2):235-42. (in persian).
50
Tartibian B, Maleki BH. The effects of honey supplementation on seminal plasma cytokines, oxidative stress biomarkers, and antioxidants during 8 weeks of intensive cycling training. J Androl. 2012;33(3):449-61.
51
Samarghandian S, Farkhondeh T, Samini F. Honey and Health: A Review of Recent Clinical Research. Pharmacognosy Res. 2017;9(2):121-7.
52
Samarghandian S, Farkhondeh T, Samini F. Honey and health: A review of recent clinical research. Pharmacogn Res. 2017;9(2):121-7.
53
Tonks AJ, Cooper R, Jones K, Blair S, Parton J, Tonks A. Honey stimulates inflammatory cytokine production from monocytes. Cytokine. 2003;21(5):242-7.
54
Buba F, Gidado A, Shugaba A. Analysis of biochemical composition of honey samples from North-East Nigeria. Biochem Anal Biochem. 2013;2(3):139-46.
55
Wang J, Vanegas SM, Du X, Noble T, Zingg J-MA, Meydani M, et al. Caloric restriction favorably impacts metabolic and immune/inflammatory profiles in obese mice but curcumin/piperine consumption adds no further benefit. Nutr Metab. 2013;10(1):29-39.
56
Sumarac-Dumanovic M, Stevanovic D, Ljubic A, Jorga J, Simic M, Stamenkovic-Pejkovic D, et al. Increased activity of interleukin-23/interleukin-17 proinflammatory axis in obese women. Int J Obes (Lond). 2009;33(1):151-6.
57
Winer S, Paltser G, Chan Y, Tsui H, Engleman E, Winer D, et al. Obesity predisposes to Th17 bias. Eur J Immunol. 2009;39(9):2629-35.
58
ORIGINAL_ARTICLE
تأثیر 12 هفته تمرین هوازی با و بدون مصرف مولتیویتامین– مینرال در هوای آلوده بر برخی عوامل ایمونولوژیک مردان سالم
هدف از انجام این مطالعه، بررسی تأثیر 12 هفته تمرین هوازی با و بدون مصرف مولتیویتامین- مینرال در هوای آلوده بر برخی عوامل ایمونولوژیک مردان سالم بود. تعداد 46 مرد سالم (سن: 1/2 ± 25 سال؛ شاخص توده بدنی: 98/0 ± 83/23 کیلوگرم بر مترمربع) بهطور تصادفی در چهار گروه تمرین هوازی همراه با مکمل (11 نفر)، تمرین هوازی بهتنهایی (11 نفر)، مکمل بهتنهایی (12 نفر) و کنترل (12 نفر) در هوای آلوده با میانگین شاخص هوای ناسالم قرار گرفتند. برنامة تمرین شامل 90 دقیقه تمرین هوازی، سه جلسه در هفته با شدت 85-60 درصد ضربان قلب ذخیرة بیشینه بهمدت 12 هفته بود. علاوهبراین، از شرکتکنندگان گروه تمرین همراه با مکمل خواسته شد که 500 میلیگرم مولتیویتامین- مینرال بهمدت 12 هفته مصرف کنند. تعداد گلبولهای سفید، نوتروفیلها، لنفوسیتها، مونوسیتها، ائوزینوفیلها و بازوفیلها، سطوح کوتیزول و ایمونوگلوبین A بزاقی بهترتیب با روشهای سل کانتر، الیزا و ایمونودیفیوزیون در حالت پایه و در پایان 12 هفته مداخله اندازه گیری شدند. دادهها با استفاده از تحلیل واریانس با اندازهگیری تکراری تجزیهوتحلیل شدند. پس از مداخله، افزایش معناداری در سلولهای ایمنی و سـطوح کـورتیزول (P = 0.047) در گروه تمرین (P = 0.01)، و کاهش معناداری در لنفوسیتها (P = 0.001)، مونوسیتها (P = 0.01) و بازوفیلها (P = 0.041) در گروه مکمل وجود داشت. همچنین، سطوح ایمونوگلوبین بزاقی آ در گروه تمرین همراه با مصرف مکمل، بهطور معناداری افزایش یافتند (P = 0.01)؛ درحالیکه در دیگر گروهها بدون تغییر ماندند. نتایج نشان داد که تمرین هوازی همراه با مصرف مکمل موجب بهبودی در برخی عوامل ایمنی در هوای آلوده میشود؛ بااینحال، برای دستیابی به نتایج قطعیتر به بررسیهای بیشتری نیاز است.
https://spj.ssrc.ac.ir/article_1607_e4974abb976629e7f91c09084a5e74a3.pdf
2019-07-23
133
154
10.22089/spj.2019.7181.1885
تمرین هوازی
مولتیویتامین مینرال
ایمنی
ایمونوگلوبین آ
کورتیزول
هوای آلوده
صابر
رضانژاد
saber.rezanejad@gmail.com
1
دانشجوی دکتری بیوشیمی و متابولیسم ورزشی، دانشگاه اصفهان، اصفهان، ایران
LEAD_AUTHOR
مهدی
کارگرفرد
m.kargarfard@spr.ui.ac.ir
2
استاد فیزیولوژی ورزشی، دانشگاه اصفهان، اصفهان، ایران
AUTHOR
عفت
بمبئی چی
e.bambaeichi@yahoo.com
3
دانشیار فیزیولوژی ورزشی، دانشگاه اصفهان، اصفهان، ایران
AUTHOR
رویا
کلیشادی
kelishadi@med.mui.ac.ir
4
استاد دانشکده پزشکی، مرکز تحقیقات رشد و نمو کودکان، دانشگاه علوم پزشکی اصفهان
AUTHOR
Kargarfard M, Poursafa P, Rezanejad S, Mousavinasab F. Effects of exercise in polluted air on the aerobic power, serum lactate level and cell blood count of active Individuals. Int J Prev Med. 2011;2:145-50.
1
Kargarfard M, Shariat A, Brandon S, Shaw ISh, Eddie TCL, Kheiri A, et al. Effects of polluted air on cardiovascular and hematological parameters after progressive maximal aerobic exercise. Lung. 2015;193:275–81.
2
Ashley MJ, Dodds L, Arbuckle T, Levy A, Platt R, Marshall J. Umbilical cord blood levels and predictors of Interleukin-33, Thymic Lymphopoietin and Immunoglobulin E. Pediatr Allergy Immunol. 2015;26:161–7.
3
Mackinnon LT. Immunology and sport. 2nd ed. Trans Mousavi T, Abdollahi M. Tehran: Imam Hossein University; 2012;120-5.
4
Gleeson M. Performance of the immune system in exercise. Trans Mohebbi H. Tehran: Publishing The World of Movement; 2008;34-9.
5
Jeremy P. Beneficial cardiovascular effects of reducing exposure to particulate air pollution with a simple facemask. Particle and Fiber Toxicology 2009; 8: 186-93.
6
Gref A, Rautiainen S, Gruzieva O, Håkansson N, Kull I, Pershagen G. Dietary total antioxidant capacity in early school age and subsequent allergic disease. Clin Exp Allergy. 2017;47:751–9.
7
Li XY, Hao L, Liu YH, Chen CY, Pai VJ, Kang JX. Protection against fine particle-induced pulmonary and systemic inflammation by omega-3 polyunsaturated fatty acids. Biochim Biophys Acta. 2017;1861:577–84.
8
Bakhtiar T, Zeinali F. Investigation the response of inflammatory, immune and hormonal indices to increasing physical activity in healthy children. Jour of Health and Care. 2016;17:154-65. (In Persian).
9
Rahmani H, Ahmadizad S, Rohani H, Nouri Habashi A, Mohammadi Dehcheshmeh M. Acute effects of continuous or high intensity interval exercise on plasma levels of E-selectin and WBC indices in CHD Pptients. JEP. 2017;35:35-48. (In Persian).
10
Mirdar Sh, Naiestany F, Hamidian Gh, Hedayati M. Increment of alveolar macrophages and pulmonary surfactant of young male rats after six weeks interval training. JEP. 2018;36:59-72. (In Persian).
11
Giahi O, Dervishi A, Sorai M, Shahsavari M. Investigation the relationship between exposure to inhaling pollutants and pulmonary function capacity in steel industry. Journal of Kurdistan University of Medical Sciences. 2016;19:135-45.
12
Ambroza A, Vikova V, Rossner P, Rossnerova A, Svecova V. Impact of air pollution on oxidative DNA damage and lipid peroxidation in mothers and their newborns. Inter Jour of Hygiene and Environ Health. 2016;219:545-56.
13
Gahani G, Abkar A, Heydari H. The effect of high-intensity intermittent Training and consumption of probiotic supplement on immune cells, reactive protein C, and immunoglobulin A in young soccer players. Jour of Qom Uni of Med Sci. 2017;10:36-46. (In Persian).
14
Cheng Sh, William D, Tang J, Brown K, Renwick S. Gleeson M. The effect of 14 weeks of vitamin D3 supplementation on antimicrobial peptides and proteins in athletes. Jour of Sports Sci. 2015; 34(1):136-46.
15
Gleeson M. Immune system adaptation in elite athletes. Current opinion in Clinical Nutrition and Metabolic car. 2006;9:659-65.
16
Gleeson M. Immune function in sport and exercise. J Appl Physiol. 2007;103:693-9.
17
Sattari Fard S, Gaini AA, Choobineh S, Shafiei L, Azami A, Adibzadeh H. Salivary immunoglobulin A changes in athletes after an exercise session in warm, cold, and natural temperatures. Hormoz Med Jour. 2014;3:239-29. (In Persian).
18
Azerbaijani MA, Nick Bakht H, Rezai MJ. The effect of continuous and periodic exercises on resting levels and rapid response of immunoglobulin A and total salivary protein in male basketball players. Jour of Kurd Med Sci. 2009;12:1-12. (In Persian).
19
Novas AM, Rowbottom DG, Jenkins DG. URTI and salivary IgA. Int J Sports Med. 2010;24:24-31.
20
Talebi K, Hejazi SM, Mottaghi MR. Investigation the effect of intense training on the concentration of immunoglobulin A and salivary cortisol swimmers. Jour of Gon of Med Sci. 2014;18:191-6. (In Persian).
21
Ebrahimi H, Nick Ravesh M. Investigation of a vigorous exhausting activity on immune globulin and salivary changes in adolescent athletic professional and recreational activities. M Q. 2006;29:147-56. (In Persian).
22
Tabarestani M, Fathi M, Attarzadeh SR, Tabarstani M. Effect of a session of aerobic exhaustion on changes in immunoglobulin A and total salivary protein in recreational athlete adolescents. Jour of Sport and Bio Sci. 2014;4:64-70. (In Persian).
23
Neville V, Gleeson M, Folland JP. Saliva IgA as a risk factor for upper respiratory infections in elite professional athletes. J Med Sci Sport Exerc. 2008; 40(7):1228-36.
24
Moreira A, Arsati F, Bosco Y, Lima AO, Franchini E, Cava AV. Effect of a kickboxing match on salivary cortisol and immunoglobulin A. Percep and Moto Skil. 2010; 111(4):158-66.
25
Shoaei T, Heidari M, Ghasemi Tehrani H, feizi A. Effects of probiotic supplementation on pancreatic β‑cell function and C‑reactive protein in women with polycystic ovary syndrome: A randomized double‑blind placebo‑controlled clinical trial. Int Jour of Pre Med. 2015;6:102-18. (In Persian).
26
Thurston GD, Jiyoung A, Kevin R. Ambient particulate matter air pollution exposure and mortality in the NIH-AARP diet and health cohort. Environ Health Pers. 2015;123:537-43.
27
Rahimi R, Ghaderi M, Mirzaei B, Ghaeni S, Faraji H, Sheikholeslami VD. Effect of very short rest periods on Immunoglobulin A and cortisol responses to resistance exercise in men. J Hum Sport Exerc. 2010;5:146-57. (In Persian).
28
Tavakoli Z, Sari Sarraf V, Amir Sasan S. The effect of exercise activity per day on acute immunoglobulin a response, cortisol, α amylase and salivary total protein in swim girls. J Met Sport. 2013;2:101-12. (In Persian).
29
Nourian J, Abed Natanzai H, Nikbakht HA. The effect of sport competition on the level of immunoglobulin A and salivary cortisol in adolescent karate. J Ilam Med Sci. 2016;23:121-8. (In Persian).
30
Safai M, Mojtahedi H, Gorbani F, Garadaghi N. Effect of 8 weeks of periodic aerobic exercise on levels of plasma A, G and M immuneglobins in obese and normal men. Sport Sci. 2017;8:221-30. (In Persian).
31
Chiodo S, Tessitore A, Cortis C, Cibelli G, Lupo C, Ammend SM. Stress related hormonal and psychological changes to official youth Taekwondo competitions. Scandinavian J Med Sci Sport. 2009; 15:1-33.
32
Limoei Ch, Hemmat Fard A, Ghofrani M, Nouri P. Comparison of the effects of exhausting exercises on serum testosterone and cortisol at two times in the morning and evening in female athletes. Sport Sci. 2012;9:33-47. (In Persian).
33
Daly W, Seeqers CA, Rubin DA, Hackney A. Relationship between stress hormones and testosterone with proloned endurance exercise. Eur J Appl Physiol. 2005;93:375-80.
34
Handziski Z, Maleska V, Petrovska S, Nikolik S. The ACTH, cortisol, testosteronel and testosteronel cortisol ratio in professional socer players during a competiton half-season. Bratisl lek lisky. 2006;107:256-63.
35
Pourvaghar MJ, Gaeini AA, Ravasi AA, Kordi MR, Shaykh Aleslam D. The effects of training time on serum immunoglobulin alterations and cortisol testosterone responses in male athlete atudents. World J of Sport Sci. 2008;1:12-6. (In Persian).
36
Zorana JA, Audrey N, Michelle AM. A study of the combined effects of physical activity and air pollution on mortality in elderly urban residents: The danish diet, cancer, and health cohort. Environ Health Pers. 2015;123:557-63.
37
Razavi Majd Z, Nazar Ali P, Hanachi P, Kordi MR. The effect of aerobic training course and vitamin D supplementation on respiratory indices in patients with asthma. J Qom University Med Sci. 2013;6:74-80. (In Persian).
38