Sport Sciences Research Institute Sport Physiology 2322-164X 9 34 2017 06 22 Relative Gene Expression of Key Genes Involved in Lipid Metabolism, Following High Fat Diet and Moderate and High Intensity Aerobic Training in Rat’s Liver Relative Gene Expression of Key Genes Involved in Lipid Metabolism, Following High Fat Diet and Moderate and High Intensity Aerobic Training in Rat’s Liver 201 216 1013 10.22089/spj.2017.2767.1374 FA Maryam Ebrahimi Ph.D. in Exercise Biochemistry and Metabolism, University of Mazandaran Rozita Fathi Associate Professor of Exercise Biochemistry and Metabolism, University of Mazandaran 0000-0002-4966-2692 Zarbakht Ansari Pirsarii Associate Professor of Animal Science, Sari Agricultural Sciences & Natural Resources University Elahe Talebi-Garakani Associate Professor of Exercise Biochemistry and Metabolism, University of Mazandaran 0000-0001-7499-8852 Journal Article 2016 07 17 To study the hepatic lipid metabolism, 44 male Wistar rats randomly divided in two normal or high fat diet groups. Each group was included of control, moderate exercise and high intensity exercise groups. After the 8 weeks of training hepatic relative gene expression of Farnesoid X Receptor (FXR), Peroxisome proliferator activated receptor alpha (PPAR-α) and also sterol regulatory element binding protein 1c (SREBP-1c) were assessed. Also, plasma lipid profile and Aminotransferases levels were measured. Data were analyzed by 2way ANOVA in SPSS 22 (P<0.05). According to the results, high fat diet caused dyslipidemia and probably liver injury and only increased SERBP-1crelative gene expression. Although training had not any effect on FXR expression, PPAR-α expression increased in trained high fat diet groups, independent to intensity. SREBP-1c expression just decreased by high intensity training in normal diet group. In summary, dyslipidemia and hepatic fat overload could be induced by high fat diet due to hepatic SREBP-1c expression and activation of lipogenic pathways. Although exercise changed the gene expression but had no effect on lipid profile or transaminases.  To study the hepatic lipid metabolism, 44 male Wistar rats randomly divided in two normal or high fat diet groups. Each group was included of control, moderate exercise and high intensity exercise groups. After the 8 weeks of training hepatic relative gene expression of Farnesoid X Receptor (FXR), Peroxisome proliferator activated receptor alpha (PPAR-α) and also sterol regulatory element binding protein 1c (SREBP-1c) were assessed. Also, plasma lipid profile and Aminotransferases levels were measured. Data were analyzed by 2way ANOVA in SPSS 22 (P<0.05). According to the results, high fat diet caused dyslipidemia and probably liver injury and only increased SERBP-1crelative gene expression. Although training had not any effect on FXR expression, PPAR-α expression increased in trained high fat diet groups, independent to intensity. SREBP-1c expression just decreased by high intensity training in normal diet group. In summary, dyslipidemia and hepatic fat overload could be induced by high fat diet due to hepatic SREBP-1c expression and activation of lipogenic pathways. Although exercise changed the gene expression but had no effect on lipid profile or transaminases.  https://spj.ssrc.ac.ir/article_1013_6b180037abbebea991d8b1232f8a8ca9.pdf