Sport Sciences Research Institute Sport Physiology 2322-164X 11 42 2019 07 23 Acute Interval Walking with Blood Flow Restriction Does Not Activate MAPK Pathway Signaling in Inactive Men Acute Interval Walking with Blood Flow Restriction Does Not Activate MAPK Pathway Signaling in Inactive Men 47 60 1526 10.22089/spj.2018.5839.1768 FA Mehdi Khoubi Ph.D. Student in Exercise Physiology, Shahid Chamran University of Ahvaz, Ahvaz, Iran, Faculty member of Payame Noor University, Tehran, Iran Abdolhamid Habibi Professor of Exercise Physiology, Shahid Chamran University of Ahvaz, Ahvaz, Iran Mmohsen Ghanbarzadeh Assistant Professor of Exercise Physiology, Shahid Chamran University of Ahvaz, Ahvaz, Iran Saeed Shakerian Associate Professor of Exercise Physiology, Shahid Chamran University of Ahvaz, Ahvaz, Iran 0000-0002-3752-8525 Bahman Mirzaie Professor of Exercise Physiology, University Of Guilan, Rasht, Iran Journal Article 2018 05 18 The purpose of the study was to investigate of effect of acute interval walking with blood flow restriction on 4EBP1, ERK and P38 proteins of skeletal muscle in inactive men. Five healthy sedentary were participated in 2 sessions including acute interval walking- BFR (5 intervals 3-min walking at 55% MHR and 1 min at rest with BFR) and acute interval walking (5 intervals 3-min walking at 55% MHR and 1 min at rest without BFR). Sampling of Vastus lateralis muscle before and 3 hours after the training from the right foot of the subjects at a distance of 5 to 10 cm. 4EBP1, ERK and P38 proteins concentration evaluated by Western blotting. Dependent t-test and and Independent t-test was used to analyze the data after subtracting the post-test score from the pre-test. However, there was a significant difference between the pre and post test for 4EBP1 (P = 0.001) and ERK (P = 0.049) in the blood flow restriction group. There was no significant difference between pre and post test of P38 (P = 0.452).significant difference was observed for ERK (P = 0.012) in acute interval walking.and (P = 0.049). There was no significant difference between pre and post test of 4EBP1(P = 0.064) and P38 (P = 0.122). No significant difference was found between two group for concentration of 4EBP1 (P=0.068), P38 (P-0.091) and ERK (P=0.827), (P ≥ 0.05). This study has shown that, Acute interval walking with blood flow restriction does not activate MAPK pathway signaling in inactive men. The purpose of the study was to investigate of effect of acute interval walking with blood flow restriction on 4EBP1, ERK and P38 proteins of skeletal muscle in inactive men. Five healthy sedentary were participated in 2 sessions including acute interval walking- BFR (5 intervals 3-min walking at 55% MHR and 1 min at rest with BFR) and acute interval walking (5 intervals 3-min walking at 55% MHR and 1 min at rest without BFR). Sampling of Vastus lateralis muscle before and 3 hours after the training from the right foot of the subjects at a distance of 5 to 10 cm. 4EBP1, ERK and P38 proteins concentration evaluated by Western blotting. Dependent t-test and and Independent t-test was used to analyze the data after subtracting the post-test score from the pre-test. However, there was a significant difference between the pre and post test for 4EBP1 (P = 0.001) and ERK (P = 0.049) in the blood flow restriction group. There was no significant difference between pre and post test of P38 (P = 0.452).significant difference was observed for ERK (P = 0.012) in acute interval walking.and (P = 0.049). There was no significant difference between pre and post test of 4EBP1(P = 0.064) and P38 (P = 0.122). No significant difference was found between two group for concentration of 4EBP1 (P=0.068), P38 (P-0.091) and ERK (P=0.827), (P ≥ 0.05). This study has shown that, Acute interval walking with blood flow restriction does not activate MAPK pathway signaling in inactive men. https://spj.ssrc.ac.ir/article_1526_e60e81c4cbe5171cd654662d9887aec2.pdf