Sport Sciences Research Institute Sport Physiology 2322-164X 12 47 2020 09 22 Alterations in Enzymatic and Non‐Enzymatic Antioxidants Levels After One Session of Repeated-Sprint Activity and Cold Water Immersion Alterations in Enzymatic and Non‐Enzymatic Antioxidants Levels After One Session of Repeated-Sprint Activity and Cold Water Immersion 133 146 1609 10.22089/spj.2017.3320.1453 FA Nima Gharahdaghi Ph.D. of Exercise Physiology, Faculty of Physical Education and Sports Sciences, University of Tehran, Iran Mohammad Reza Kordi Associate Professor of Exercise Physiology, Faculty of Physical Education and Sports Sciences, University of Tehran, Iran 0000-0002-7999-4292 Fatemeh Shabkhiz Associate Professor of Exercise Physiology, Faculty of Physical Education and Sports Sciences, University of Tehran, Iran 0000-0003-1183-9517 Sara Farajnia M.Sc. of Exercise Physiology, Faculty of Physical Education and Sports Sciences, University of Tehran, Iran Mojtaba Khaki Ph.D. Student of Exercise Physiology, Faculty of Physical Education and Sports Sciences, University of Tehran, Iran Saman Hajizadeh M.Sc. of Exercise Physiology, Faculty of Physical Education and Sports Sciences, University of Tehran, Iran Journal Article 2016 11 16 The purpose of this study was to measure the changes in oxidized (GSSG) and reduced (GSH) glutathione, glutathione reductase (GR) and glutathione peroxidase (GPX) after repeated-sprint activity (RSA) and subsequent cold water immersion (CWI). Twenty trained athletes with maximal oxygen uptake (VO_2max) 52.9±2.9 ml.kg^-1.min^-1, age 21.9±2.2 yrs, height 174.2±5.4 cm, and weight 68±4.4 kg was assigned to take part in this study. After performing repeated-sprint activity, 10 participants immersed in cold water (14°c) and 10 participants passively sat on a chair at room temperature. Blood sampling was performed before and after RSA, after CWI or passive rest and after 24 h. The blood variables assessed through ELISA method. The results showed that antioxidant levels did not return to baseline levels and CWI had no effect on these factors, compared to GPX and GR returned to baseline levels after 24 h, nevertheless; there was no significant difference between CWI and Control groups. In conclusion, although the levels of enzymatic and non‐enzymatic antioxidants were significantly higher after RSA, CWI did not have any effect on returning to baseline levels after 24h. The purpose of this study was to measure the changes in oxidized (GSSG) and reduced (GSH) glutathione, glutathione reductase (GR) and glutathione peroxidase (GPX) after repeated-sprint activity (RSA) and subsequent cold water immersion (CWI). Twenty trained athletes with maximal oxygen uptake (VO_2max) 52.9±2.9 ml.kg^-1.min^-1, age 21.9±2.2 yrs, height 174.2±5.4 cm, and weight 68±4.4 kg was assigned to take part in this study. After performing repeated-sprint activity, 10 participants immersed in cold water (14°c) and 10 participants passively sat on a chair at room temperature. Blood sampling was performed before and after RSA, after CWI or passive rest and after 24 h. The blood variables assessed through ELISA method. The results showed that antioxidant levels did not return to baseline levels and CWI had no effect on these factors, compared to GPX and GR returned to baseline levels after 24 h, nevertheless; there was no significant difference between CWI and Control groups. In conclusion, although the levels of enzymatic and non‐enzymatic antioxidants were significantly higher after RSA, CWI did not have any effect on returning to baseline levels after 24h. https://spj.ssrc.ac.ir/article_1609_a2cc63e065705fe938a4dda49092966f.pdf