Sport Sciences Research Institute Sport Physiology 2322-164X 6 22 2014 07 23 The effect of endurance training on skeletal muscle sodium bicarbonate cotransporter1(NBC1) and sodium proton exchanger1(NHE1) gene expression in rat The effect of endurance training on skeletal muscle sodium bicarbonate cotransporter1(NBC1) and sodium proton exchanger1(NHE1) gene expression in rat 55 68 261 FA A Monazzami H Rajabi 0000-0002-3276-1598 R Ghrakhanlou Journal Article 2013 07 22 The purpose of this study was to investigate the effect of endurance training on muscle NHE1 and NBC1 gene expression in rat. Male vistar rats (n=20), 4weeks age and 95.7±10.8g in weight were selected and divided into control and training groups randomly. Endurance training were performed for 7weeks that be started with relatively low speed and duration (20m∕min and 20 min respectively) in the first week and reached to 30m∕min and 35min gradually in the last week. NHE1 and NBC1 gene expression determined by Real time-PCR technique in Soleus as a red (oxidative) and EDL as a white (glycolytic) muscle preparation. The between groups differences in variables were determined by independent t-test through REST software .NHE1 mRNA increased in EDL and Soleus by 74% and 51% in trained group in compare with control respectively (P<0/05). NBC1 mRNA expression was also increased in EDL and Soleus by 62% and 41% in trained group, respectively, but not significant in Soleus (P<0/05). In conclusion, these result showed that endurance training  increased NHE1 and NBC1 mRNA expression in both oxidative and glycolytic fibers , Therefore endurance training may improve the capacity for pHi regulation in muscles by lactate independent pathway and  these  increases depend  on transporter and fiber-type specify that are most important in pH regulation. The purpose of this study was to investigate the effect of endurance training on muscle NHE1 and NBC1 gene expression in rat. Male vistar rats (n=20), 4weeks age and 95.7±10.8g in weight were selected and divided into control and training groups randomly. Endurance training were performed for 7weeks that be started with relatively low speed and duration (20m∕min and 20 min respectively) in the first week and reached to 30m∕min and 35min gradually in the last week. NHE1 and NBC1 gene expression determined by Real time-PCR technique in Soleus as a red (oxidative) and EDL as a white (glycolytic) muscle preparation. The between groups differences in variables were determined by independent t-test through REST software .NHE1 mRNA increased in EDL and Soleus by 74% and 51% in trained group in compare with control respectively (P<0/05). NBC1 mRNA expression was also increased in EDL and Soleus by 62% and 41% in trained group, respectively, but not significant in Soleus (P<0/05). In conclusion, these result showed that endurance training  increased NHE1 and NBC1 mRNA expression in both oxidative and glycolytic fibers , Therefore endurance training may improve the capacity for pHi regulation in muscles by lactate independent pathway and  these  increases depend  on transporter and fiber-type specify that are most important in pH regulation. https://spj.ssrc.ac.ir/article_261_b1a59b315fc9a3002ce38bbe070ec3f5.pdf