Sport Sciences Research Institute Sport Physiology 2322-164X 15 60 2023 12 22 The effect of eight weeks endurance training on Sca-1 gene expression in the heart of male rats The effect of eight weeks endurance training on Sca-1 gene expression in the heart of male rats 107 120 4368 10.22089/spj.2024.16731.2311 FA Mahboobeh Borjian Fard Department of Exercise Physiology, Faculty of Sport Sciences and Health, University of Tehran, Tehran, Iran 0000-0003-0468-6030 Journal Article 2023 06 22 Objective: The aim of the present study was to investigate the effect of eight weeks of endurance exercise on the expression of the Stem cells antigen-1 (Sca-1) gene in cardiac hypertrophy of male Wistar rats. Methods and materials: In this experimental study, 16 adult male Wistar rats were divided into two groups of eight: control and endurance training. The endurance training group performed continuous treadmill running for 50 minutes, five days a week, at an intensity of 65-70% Vo2 max for a period of eight weeks. The control group did not engage in any exercise during this time. After 48 hours following the last training session, the rats were dissected. The heart-to-body weight ratio and left ventricular wall thickness were measured to assess cardiac hypertrophy in the Wistar rats. The expression level of the Sca-1 gene in the heart was also measured using Real-time PCR. In the end, the collected data were evaluated using independent t-test at a significance level of 0.05. Results The results showed that both heart-to-body weight ratio and left ventricular wall thickness were significantly higher in the endurance exercise group compared to the control group. Additionally, Sca-1 levels were significantly increased in the endurance exercise group compared to the control group. Conclusions: It appears that endurance exercise can have prominent effects on cardiac hypertrophy by regulating an increase in Sca-1 expression Objective: The aim of the present study was to investigate the effect of eight weeks of endurance exercise on the expression of the Stem cells antigen-1 (Sca-1) gene in cardiac hypertrophy of male Wistar rats. Methods and materials: In this experimental study, 16 adult male Wistar rats were divided into two groups of eight: control and endurance training. The endurance training group performed continuous treadmill running for 50 minutes, five days a week, at an intensity of 65-70% Vo2 max for a period of eight weeks. The control group did not engage in any exercise during this time. After 48 hours following the last training session, the rats were dissected. The heart-to-body weight ratio and left ventricular wall thickness were measured to assess cardiac hypertrophy in the Wistar rats. The expression level of the Sca-1 gene in the heart was also measured using Real-time PCR. In the end, the collected data were evaluated using independent t-test at a significance level of 0.05. Results The results showed that both heart-to-body weight ratio and left ventricular wall thickness were significantly higher in the endurance exercise group compared to the control group. Additionally, Sca-1 levels were significantly increased in the endurance exercise group compared to the control group. Conclusions: It appears that endurance exercise can have prominent effects on cardiac hypertrophy by regulating an increase in Sca-1 expression https://spj.ssrc.ac.ir/article_4368_ae0909a324fb2530e205e52d40266418.pdf