Sport Sciences Research Institute Sport Physiology 2322-164X 7 28 2016 02 20 The effect of 14 weeks of endurance training on miR-499 expression of left ventricle in wistar male rat The effect of 14 weeks of endurance training on miR-499 expression of left ventricle in wistar male rat 59 72 587 FA Mohammad Fathi 0000-0002-2113-365X Saeed Abroun Journal Article 2014 09 01 The aim of this study was to investigate the effect of endurance training on cardiac expression of miR-499, For this purpose, 14 rats under controlled conditions (temperature, light/dark (12:12) cycle, with ad Libitum access to food and water) were housed and after familiarization with protocol they were randomly assigned into control and Experimental groups. The experimental group performed 14 weeks endurance exercise on motorized treadmill, and then 48 hours after the end of the last session were anesthetized and sacrificed. The heart was removed and then left ventricle was dissected. Real time RT-PCR method was used to determine of expression levels of miR-449 in left ventricle and the obtained data were evaluated using one sample t-test. The hypertrophy evaluation indices showed that the ratio of left ventricle to the body weight in experimental group (2.3±0.18) was significantly (P=0.05) higher in compare control group (2.049±0.12) and the ratio of left ventricle to body surface area in experimental group (0.168±0.008) were significantly (P=0.01) higher in compare with control group (0.153±0.006). Finally, the mean of miR-499 expression of left ventricle in experimental group was significantly (P=0.004) higher than control group. It seems miR-499 expression relate to endurance induce cardiac hypertrophy special in left ventricle.  The aim of this study was to investigate the effect of endurance training on cardiac expression of miR-499, For this purpose, 14 rats under controlled conditions (temperature, light/dark (12:12) cycle, with ad Libitum access to food and water) were housed and after familiarization with protocol they were randomly assigned into control and Experimental groups. The experimental group performed 14 weeks endurance exercise on motorized treadmill, and then 48 hours after the end of the last session were anesthetized and sacrificed. The heart was removed and then left ventricle was dissected. Real time RT-PCR method was used to determine of expression levels of miR-449 in left ventricle and the obtained data were evaluated using one sample t-test. The hypertrophy evaluation indices showed that the ratio of left ventricle to the body weight in experimental group (2.3±0.18) was significantly (P=0.05) higher in compare control group (2.049±0.12) and the ratio of left ventricle to body surface area in experimental group (0.168±0.008) were significantly (P=0.01) higher in compare with control group (0.153±0.006). Finally, the mean of miR-499 expression of left ventricle in experimental group was significantly (P=0.004) higher than control group. It seems miR-499 expression relate to endurance induce cardiac hypertrophy special in left ventricle.  https://spj.ssrc.ac.ir/article_587_766ebcd59621e305170616ba3d3dac32.pdf