The Effect of Boldenone and Resistance Training on Hematological Profile and Spleen Structure in Wistar Rats

Document Type : Research Paper

Authors
Asieh Abbassi Daloii 1
Ahmad Abdi 1
Seyed Javad Ziaolhagh 2
Asad Shahbahrami 3
1 Assistant Professor of Sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2 Assistant Professor of Sport Physiology, Shahrood Branch, Islamic Azad University, Shahrood, Iran
3 M. Sc. of Sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
10.22089/spj.2017.2366.1319
Abstract
The aim of this study was to investigate the effect of boldenone and Resistance training on Hematological profile and spleen structure in Wistar rats. 47 male Wistar rats aged 12 weeks (weight 195±7.94 g) were randomly divided into seven groups: control, sham, boldenone-1, boldenone-2, Resistance training, Resistance training+boldenone-1, Resistance training+boldenone-2. Resistance training program includes six weeks of climbing a ladder for 5 sessions, 3 sets, 5 repetitions, and one-minute resting between sets with 50% 1RM in the first week and 100% 1RM in the final week. Injection once a week, on an appointed day, and in the quadriceps and hamstring was conducted in depth. After anesthesia, autopsy was performed and the spleen Isolated. Selected microscopic sections, then stained with hematoxylin and eosin were studied with microscopic. Spleen injury was determined based on histological criteria. Data were analyzed by T-Test, one-way ANOVA and post hoc LSD at the significant level P
Keywords
Resistance Training
Boldenone
Hematological factors
Spleen Structure Changes
Wistar Rats

Main Subjects

  1. Lumia A R, McGinnis M Y. Impact of anabolic androgenic steroids on adolescent males. Physiol Behav. 2010;100(3):199-204.2010; 100-‌‌99.
  2. Guan F, Cornelius E U, Soma R, Youwen Y, Liu Y, Lia X. High-throughput UHPLC MS/ MSmethod for the detection, quantification and identification of fifty-five anabolic and androgenic steroids in equine plasma. J Mass Spectrom. 2010; 45(11): 1270-9.
  3. Kicman A T. Pharmacology of anabolic steroids. Br J Pharmacol 2008;154(3):502-21. 2008; 154-502.
  4. Velazquez I, Alter B P. Androgens and liver tumors: Fanconi’s anemia and non-Fanconi’s anemia. Am J Hematol. 2004; 77: 257-67.
  5. Standring S. Gray’s anatomy: The anatomical basis of the clinical practice. 39th ed‌. Edinburg: Elsevier Churchill Livingstone; 2006. p. 1239-44.
  6. Sandler M P, Mrvin W, Kronenberg F, Mervyn B, Oscar H, Jeffrey A, et al. Dynamic fluctuations in blood and spleen radioactivity: Splenic contraction and relation to clinical radionuclide. Journal of the American College of Cardiology, 1984; 3(5): 1205-11.
  7. Alm-Eldeen A, Tousson E. Deterioration of glomerular endothelial surface layer and the alteration in the renal function in Rabbits after treatment with a growth promoter Boldenone. Human and Experimental Toxicology. 2012; 31(5): 465-72.
  8. Groot M J, Biolatti B. Histopathological effects of boldenone in cattle. J Vet Med. 2004; 51: 58–63.
  9. Stewart I B, McKenzie D C. The human spleen during physiological stress. Sports Med. 2002; 32(6): 361-9.
  10. Alén M. Androgenic steroid effects on liver and red cells. Br J Sports Med. 1985; 19: 15-20.
  11. Hartgens F, Hamulyak K, Pernot C. Effects of high doses androgenic-anabolic steroids on haematologic parameters in bodybuilders. Abstract from the 8th FIMS European sports medicine congress; Granada, Spain, 1995. P. 23-7.
  12. Fernández M D, Fuente M L, Fernfindez E, Manso R. Anabolic steroids and lymphocyte function in sedentary and exercise-trained rats. Steroid Biochem Molec Biol. 1996; 59: 225-32.
  13. Qadampour Vahed Z, Rashidlamir A, Moosavi Z, Raji A R. The effects of anabolic steroid stanozolol along with eight weeks of resistance training on structural changes in male rats' liver. Sport Biosciences. 2013; 5(2): 115-32. (In Persian).
  14. Ozdemir M, Yalcin H. The effects of testosterone on certain organs and the macro-anatomical changes induced by this hormone in pubescent male and female rats. Movement and Health. 2012; 3(1): 83-8.
  15. Sukho L, Roger P. Resistance training, muscle mass and function in the Rat. An International Electronic Journal. 2003; 6(2): 1097-9751.
  16. Ahmed N F, Neamat A. Effect of boldenone undecylenate on haematological and biochemical parameters in veal calves. Global Veterinaria. 2014; 13‌(6): 1092-‌96.
  17. Urhausen A, Torsten A, Wilfried K. Reversibility of the effects on blood cells, lipids, liver function and hormones in former anabolic-androgenic steroid abusers. J Steroid Biochem Mol Biol. 2003; 84(2-3): 369-75.
  18. Saad A H, Torroba M, Varas A, Zapata A. Testosterone induces lymphopenia in turtles. Vet Immunol Immunopathol. 1991; 28(2): 173-80.
  19. Nakao A, Sakagami K, Nakata Y, Komazawa K, Amimoto T, Nakashima K, et al. Multiplehepatic adenomas caused by long-termad ministration of androgenic steroids for a plasticanemia in association with familial adenomatouspolyposis. Journal of Gastroenterology. 2000; 35: 557-62.
  20. Blystone C R, Furr J, Lambright C S, Howdeshell K L, Ryan B C, Wilson V S, et al. Prochloraz inhibits testosterone production at dosages below those that affect androgen-dependent organ weights or the onset of puberty in the male sprague dawley rat. Toxicologıcal Sciences. 2007; 97(1): 65-74.
  21. Balkaya M, Metin K, Kozaci D, Hümeyra Ü. Erkek Wistar ratlarında (rattus norvegicus) kalitatif ve kantitatif protein yetersizliklerinin canlı ağırlık ve bazı organ ağırlıkları uzerine etkileri. Fen Bilimleri Dergisi. 2002; 23‌(1): 48-60.
  22. Parkinson A B, Evans N A. Anabolic androgenic steroid: A survey of 500 users. Medicine Science in Sports & Exercise. 2006; 38(4): 644-51.
  23. Babaei A L, Arshami J L, Haghparast A, Danesh Mesgaran M L. Effects of saffron (crocus sativus) petal ethanolic extract on hematology, antibody response, and spleen histology in rats. Avicenna J Phytomed. 2014; 4(2): 103-9.                 (In Persian).
  24. Gabr I, Hassan T A, Abo El-M, Amal M, Aotifa A M. Effects of growth promoter Boldenone undecylenate on weaned male lambs. Nature and Science. 2009; 7(3): 61-9.
  25. Kutscher E C, Lund B C, Perry P J. Anabolic steroids: A review for the clinician. Sports Med Review. 2002; 32(5): 285-96.
Sport Physiology
Volume 9, Issue 34 - Serial Number 34
Summer 2017
July 2017
Pages 129-146

  • Receive Date 08 April 2016
  • Revise Date 18 June 2016
  • Accept Date 28 June 2016