Relative Gene Expression of Key Genes Involved in Lipid Metabolism, Following High Fat Diet and Moderate and High Intensity Aerobic Training in Rat’s Liver

Document Type : Research Paper

Authors
Maryam Ebrahimi 1
Rozita Fathi 2
Zarbakht Ansari Pirsarii 3
Elahe Talebi-Garakani 2
1 Ph.D. in Exercise Biochemistry and Metabolism, University of Mazandaran
2 Associate Professor of Exercise Biochemistry and Metabolism, University of Mazandaran
3 Associate Professor of Animal Science, Sari Agricultural Sciences & Natural Resources University
10.22089/spj.2017.2767.1374
Abstract
To study the hepatic lipid metabolism, 44 male Wistar rats randomly divided in two normal or high fat diet groups. Each group was included of control, moderate exercise and high intensity exercise groups. After the 8 weeks of training hepatic relative gene expression of Farnesoid X Receptor (FXR), Peroxisome proliferator activated receptor alpha (PPAR-α) and also sterol regulatory element binding protein 1c (SREBP-1c) were assessed. Also, plasma lipid profile and Aminotransferases levels were measured. Data were analyzed by 2way ANOVA in SPSS 22 (P<0.05). According to the results, high fat diet caused dyslipidemia and probably liver injury and only increased SERBP-1crelative gene expression. Although training had not any effect on FXR expression, PPAR-α expression increased in trained high fat diet groups, independent to intensity. SREBP-1c expression just decreased by high intensity training in normal diet group. In summary, dyslipidemia and hepatic fat overload could be induced by high fat diet due to hepatic SREBP-1c expression and activation of lipogenic pathways. Although exercise changed the gene expression but had no effect on lipid profile or transaminases. 
Keywords
High Fat Diet
Aerobic Training
FXR
PPAR-α
SREBP-1c

Main Subjects

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Sport Physiology
Volume 9, Issue 34 - Serial Number 34
Summer 2017
July 2017
Pages 201-216

  • Receive Date 17 July 2016
  • Revise Date 03 October 2016
  • Accept Date 15 October 2016