The Effect of High Intensity Interval Training (HIIT) on Gene Expression of Uncoupling Protein 1 (UCP-1) in Subcutaneous White Adipose Tissue of Wistar Rats

Document Type : Research Paper

Authors

1 M.Sc. in Exercise Physiology, University of Tehran

2 Associate Professor of Exercise Physiology, University of Tehran

3 Professor of Immunogenetics, Tehran University of Medical Sciences

4 Assistant Professor of Exercise Physiology, University of Ayatollah Alozma Boroujerdi

Abstract
The purpose of this study was to investigate the effect of High Intensity Interval Training (HIIT) on gene expression of Uncoupling Protein 1 (UCP-1) in subcutaneous white adipose tissue of wistar rats. For this purpose,24 rats were divided into three groups included: 1) Control (n=8) 2) Moderate volume HIIT (n=8) and 3) high volume HIIT (n=8). The subjects of training groups underwent two models of HIIT at different volume (moderate and high) for 8 weeks (5 sessions per week). Forty-eight hours after last training session, rats were euthanized and subcutaneous adipose tissue (inguinal depot) were quickly dissected out. The Syber Green Real Time–PCR method were used to measure the gene expression of UCP-1. One-way analysis of variance (ANOVA) was used to analysis of data by a p value of P

Keywords

Main Subjects

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Sport Physiology
Volume 10, Issue 39
Fall 2018
November 2019
Pages 17-32

  • Receive Date 24 April 2016
  • Revise Date 18 October 2016
  • Accept Date 24 October 2016