Sport Physiology

Sport Physiology

Effects of Aerobic Exercise and Calorie Restriction (Low-Carb Diet and Standard Diet) on Adipokines Affecting Insulin Resistance in Rats with Type 2 Diabetes

Document Type : Research Paper

Authors
Hosein Dastbarhagh 1
Mehdi Kargar Fard 2
Effat Bambaechi 3
Hassan Ali Abedi 4
1 Ph.D. Students at Sport Physiology, University of Isfahan
2 Professor of Sport Physiology, University of Isfahan
3 Associate Professor of Sport Physiology, University of Isfahan
4 Assistant Professor of Medicine Physiology, Jahroom University of Medical Sciences
10.22089/spj.2017.4283.1572
Abstract
The aim of this study was to evaluate the interactive effects of calorie restriction (standard diet and low-carb diet) in combination with aerobic exercise on the adipokines that are affective on insulin resistance in type 2 diabetic male rats. In an experimental study, 48 male Wistar rats (age 10 weeks, weight 229±19.5g) were randomly divided equally into six groups after diabetes induction. Group1: Diabetic control, group 2: 20% negative balance calorie with aerobic exercise, group 3: 20% caloric restriction with standard diet, group4: 20% caloric restriction with low carbohydrate diet, group 5: 20% caloric restriction with standard diet + aerobic exercise and group6: 20% calorie restriction with low-carb diet + aerobic exercise. Then 20% of negative energy balance was carried out on a daily basis of all groups for 8 weeks. Results showed that exercise alone and in combination with low-carb diet caused a significant increase in the Omentin-1 compared to diabetic control group (P≤0.05). But no significant differences were observed in the chemerin between the experimental groups with diabetic control group. The results also showed that the standard diet group and the low-carbohydrate diet alone and in combination with exercise were significantly improved insulin resistance (P≤0.05). This study showed that combination of aerobic exercise and low carbohydrates diet can increase Omentin-1 and improve insulin sensitivity in rats with type 2 diabetes.
Keywords
Omentin-1
Chemerin
Insulin Resistance
Exercise
Diet
Calorie Restriction
Subjects
  1. Lizcano F, Guzmán G. Estrogen deficiency and the origin of obesity during menopause. BioMed research international. Volume 2014, Article ID 757461, 11 pages.
  2. Tan BK, Adya R, Farhatullah S, Lewandowski KC, O’Hare P, Lehnert H, et al. Omentin-1, a novel adipokine, is decreased in overweight insulin-resistant women with polycystic ovary syndrome. Diabetes. 2008; 57(4): 801-8.
  3. Roman AA, Parlee SD, Sinal CJ. Chemerin: a potential endocrine link between obesity and type 2 diabetes. Endocrine. 2012; 42(2): 243-51.
  4. Conde J, Scotece M, Gómez R, López V, Gómez‐Reino JJ, Lago F, et al. Adipokines: biofactors from white adipose tissue. A complex hub among inflammation, metabolism, and immunity. Biofactors. 2011; 37(6): 413-20.
  5. Ouchi N, Parker JL, Lugus JJ, Walsh K. Adipokines in inflammation and metabolic disease. Nature Reviews Immunology. 2011; 11(2): 85-97.
  6. Shoelson SE, Lee J, Goldfine AB. Inflammation and insulin resistance. The Journal of clinical investigation. 2006; 116(7): 1793-801.
  7. Goldberg RB. Cytokine and cytokine-like inflammation markers, endothelial dysfunction, and imbalanced coagulation in development of diabetes and its complications. The Journal of Clinical Endocrinology & Metabolism. 2009; 94(9): 3171-82.
  8. De Souza Batista CM, Yang R-Z, Lee M-J, Glynn NM, Yu D-Z, Pray J, et al. Omentin plasma levels and gene expression are decreased in obesity. Diabetes. 2007; 56(6): 1655-61.
  9. Moreno-Navarrete JM, Catalán V, Ortega F, Gómez-Ambrosi J, Ricart W, Frühbeck G, et al. Circulating omentin concentration increases after weight loss. Nutrition & metabolism. 2010; 7(1): 1.
  10. Giannopoulou I, Fernhall B, Carhart R, Weinstock RS, Baynard T, Figueroa A, et al. Effects of diet and/or exercise on the adipocytokine and inflammatory cytokine levels of postmenopausal women with type 2 diabetes. Metabolism. 2005; 54(7): 866-75.
  11. Cantó C, Auwerx J. Caloric restriction, SIRT1 and longevity. Trends in Endocrinology & Metabolism. 2009; 20(7): 325-31.
  12. Weindruch R. Caloric restriction: life span extension and retardation of brain aging. Clinical Neuroscience Research. 2003; 2(5): 279-84.
  13. Masoro EJ. Dietary restriction and longevity extension as a manifestation of hormesis. Human and Ecological Risk Assessment. 2000; 6(2): 273-9.
  14. Cartee G, Kietzke E, Briggs-Tung C. Adaptation of muscle glucose transport with caloric restriction in adult, middle-aged, and old rats. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 1994; 266(5): R1443-R1447.
  15. Fathi R, Aslani moghanjoughi S, Talebi Garakani E, Safarzadeh A, Seyghal H. Effect of 8-week resistance training on plasma vitamin levels and its relation to insulin male rats. Iranian Journal of Diabetes and Lipid Disorders. 2015; 14(6): 390-8.
  16. Khalafi M, Fatemeh Shabkhiz S, Zolfaghari M, Zarei Y. The Effect of Two Types of Exercise on Serum Chemerin in Diabetic Male Rats. Qom Univ Med Sci J. 2016; 10(8): 27-35.
  17. Feinman RD, Volek JS. Low carbohydrate diets improve atherogenic dyslipidemia even in the absence of weight loss. Nutrition & metabolism. 2006; 3(1): 24.
  18. Krauss RM, Blanche PJ, Rawlings RS, Fernstrom HS, Williams PT. Separate effects of reduced carbohydrate intake and weight loss on atherogenic dyslipidemia. The American journal of clinical nutrition. 2006; 83(5): 1025-31.
  19. Volek JS, Feinman RD. Carbohydrate restriction improves the features of Metabolic Syndrome. Metabolic Syndrome may be defined by the response to carbohydrate restriction. Nutrition & metabolism. 2005; 2(1): 31.
  20. Volek JS, Fernandez ML, Feinman RD, Phinney SD. Dietary carbohydrate restriction induces a unique metabolic state positively affecting atherogenic dyslipidemia, fatty acid partitioning, and metabolic syndrome. Progress in lipid research. 2008; 47(5): 307-18.
  21. Liu WY, Du XM, Sun JQ, Ge J, Wang RW, Wang R, et al. Effect of aerobic exercise and low carbohydrate diet on pre-diabetic non-alcoholic fatty liver disease in postmenopausal women and middle aged men–the role of gut microbiota composition: study protocol for the AELC randomized controlled trial. BMC public health. 2014; 14(1): 1.
  22. Ruth MR, Port AM, Shah M, Bourland AC, Istfan NW, Nelson KP, et al. Consuming a hypocaloric high fat low carbohydrate diet for 12weeks lowers C-reactive protein, and raises serum adiponectin and high density lipoprotein-cholesterol in obese subjects. Metabolism. 2013; 62(12): 1779-87.
  23. Ryan A, Nicklas B, Berman D, Elahi D. Adiponectin levels do not change with moderate dietary induced weight loss and exercise in obese postmenopausal women. International journal of obesity. 2003; 27(9): 1066-71.
  24. Pierre W, Gildas AJH, Ulrich MC, Modeste W-N, azTélesphore Benoît N, Albert K. Hypoglycemic and hypolipidemic effects of Bersama engleriana leaves in nicotinamide/streptozotocin-induced type 2 diabetic rats. BMC complementary and alternative medicine. 2012; 12(1): 264.
  25. Masiello P, Broca C, Gross R, Roye M, Manteghetti M, Hillaire-Buys D, et al. Experimental NIDDM: development of a new model in adult rats administered streptozotocin and nicotinamide. Diabetes. 1998; 47(2): 224-229.
  26. Shirwaikar A, Rajendran K, Barik R. Effect of aqueous bark extract of Garuga pinnata Roxb. in streptozotocin-nicotinamide induced type-II diabetes mellitus. Journal of ethnopharmacology. 2006; 107(2): 285-90.
  27. Reeves PG, Nielsen FH, Fahey Jr GC. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J nutr. 1993; 123(11): 1939-51.
  28. Chen J-H, Ouyang C, Ding Q, Song J, Cao W, Mao L. A moderate low-carbohydrate low-calorie diet improves lipid profile, insulin sensitivity and adiponectin expression in rats. Nutrients. 2015; 7(6): 4724-38.
  29. Jashni HK, Mohebbi H, Delpasand A, Jahromy HK. Caloric restriction and exercise training, combined, not solely improve total plasma adiponectin and glucose homeostasis in streptozocin-induced diabetic rats. Sport Sciences for Health. 2015; 11(1): 81-6.
  30. Shepherd R, Gollnick P. Oxygen uptake of rats at different work intensities. Pflügers Archiv European Journal of Physiology. 1976; 362(3): 219-22.
  31. Garekani ET, Mohebbi H, Kraemer RR, Fathi R. Exercise training intensity/volume affects plasma and tissue adiponectin concentrations in the male rat. Peptides. 2011; 32(5): 1008-12.
  32. Tapan S, Tascilar E, Abaci A, Sonmez A, Kilic S, Erbil MK, et al. Decreased plasma apelin levels in pubertal obese children. Journal of Pediatric Endocrinology and Metabolism. 2010; 23(10): 1039-46.
  33. Arora SK, McFarlane SI. The case for low carbohydrate diets in diabetes management. Nutrition & metabolism. 2005; 2(1): 16.
  34. Goodwin ML. Blood glucose regulation during prolonged, submaximal, continuous exercise: a guide for clinicians. Journal of diabetes science and technology. 2010; 4(3): 694-705.
  35. Suh S-H, Paik I-Y, Jacobs K. Regulation of blood glucose homeostasis during prolonged. Mol cells. 2007; 23: 272-9.
  36. Taddibi V, Rahimi M, Comparing the effect of two protocols concurrent training (strength-aerobic) on fasting blood glucose, glycosylated hemoglobin, high-sensitivity C - reactive protein and insulin resistance in women with type 2 diabetes, Eexrcise physiology, spring 2017;2(2):95-100.
  37. Masoro EJ, McCarter RJ, Katz MS, McMahan CA. Dietary restriction alters characteristics of glucose fuel use. Journal of gerontology. 1992; 47(6): 202-8.
  38. Wetter TJ, Gazdag AC, Dean DJ, Cartee GD. Effect of calorie restriction on in vivo glucose metabolism by individual tissues in rats. American Journal of Physiology-Endocrinology And Metabolism. 1999; 276(4): 728-38.
  39. Blades M, Morgan J, Dickerson J. Dietary advice in the management of diabetes mellitus-history and current practice. The Journal of the Royal Society for the Promotion of Health. 1997; 117(3): 143-50.
  40. Group, D. S. and E. D. E. Group (1999). "Glucose tolerance and mortality: comparison of WHO and American Diabetes Association diagnostic criteria." Lancet 354: 617-621.
  41. Sheard NF, Clark NG, Brand-Miller JC, Franz MJ, Pi-Sunyer FX, Mayer-Davis E, et al. Dietary carbohydrate (amount and type) in the prevention and management of diabetes. Diabetes care. 2004; 27(9): 2266-71.
  42. Gannon MC, Nuttall FQ, Saeed A, Jordan K, Hoover H. An increase in dietary protein improves the blood glucose response in persons with type 2 diabetes. The American journal of clinical nutrition. 2003; 78(4): 734-41.
  43. Boden G, Sargrad K, Homko C, Mozzoli M, Stein TP. Effect of a low-carbohydrate diet on appetite, blood glucose levels, and insulin resistance in obese patients with type 2 diabetes. Annals of internal medicine. 2005; 142(6): 403-11.
  44. Cai R, Wei L, Di J, Yu H, Bao Y, Jia W. Expression of omentin in adipose tissues in obese and type 2 diabetic patients. Zhonghua yi xue za zhi. 2009; 89(6): 381-4.
  45. Gürsoy G, Kırnap N, Eşbah O, Acar Y, Demirbaş B, Akçayöz S, et al. The relationship between plasma omentin-1 levels and insulin resistance in newly diagnosed type 2 diabetıc women. Clinical reviews and opinions. 2010; 2(4): 49-54.
  46. Bessesen DH. The role of carbohydrates in insulin resistance. The Journal of nutrition. 2001; 131(10): 2782-6.
  47. Foster GD, Wyatt HR, Hill JO, McGuckin BG, Brill C, Mohammed BS, et al. A randomized trial of a low-carbohydrate diet for obesity. New England Journal of Medicine. 2003; 348(21): 2082-90.
  48. Ginsberg HN. Insulin resistance and cardiovascular disease. The Journal of clinical investigation. 2000; 106(4): 453-8.
  49. Bozaoglu K, Bolton K, McMillan J, Zimmet P, Jowett J, Collier G, et al. Chemerin is a novel adipokine associated with obesity and metabolic syndrome. Endocrinology. 2007; 148(10): 4687-94.
  50. Sell H, Laurencikiene J, Taube A, Eckardt K, Cramer A, Horrighs A, et al. Chemerin is a novel adipocyte-derived factor inducing insulin resistance in primary human skeletal muscle cells. Diabetes. 2009; 58(12): 2731-40.
  51. Venojärvi M, Wasenius N, Manderoos S, Heinonen OJ, Hernelahti M, Lindholm H, et al. Nordic walking decreased circulating chemerin and leptin concentrations in middle-aged men with impaired glucose regulation. Annals of medicine. 2013; 45(2): 162-70.
  52. Alfadda, A. A., R. M. Sallam, et al.. "Differential patterns of serum concentration and adipose tissue expression of chemerin in obesity: adipose depot specificity and gender dimorphism." Molecules and cells 2012; 33(6): 591-596
Sport Physiology
Volume 9, Issue 36 - Serial Number 36
Winter 2018
Winter 2018
Pages 173-188

  • Receive Date 05 June 2017
  • Revise Date 05 September 2017
  • Accept Date 18 September 2017