Document Type : Research Paper
Authors
1 Ph.D. in Sport Physiology, University of Mazandaran
2 Professor Sport Physiology, University of Mazandaran
3 Assistant Professor of Sport Physiology, Shahed University, Tehran
Abstract
Considering the important role of inflammation in pulmonary disorders the purpose of this study was to investigate the effect of a period of swimming training on Tumor necrosis factor alpha (TNF-α)and Nuclear factor- kappa B (NF-κB) gene expression in lung tissue of wistar rats. Twenty wistar rats of five weeks ages with the weight range of 102±23 g randomly divided into control (n=10) and swimming training groups (n=10). The training protocol included three months of swimming (5 times per week) which begun from 25 minutes in the first week (with the water flow intensity of 4 liters per minute) and reached to 60 minutes (with the water flow intensity of 10 liters per minute) at the final week. Quantitative Real-Time PCR used for detecting and quantifying expression profiles of TNF-αand NF-κB genes in the lung tissue of rats. Kolmogorov-Smirnov test was used to test the normality of data distribution and Independent t test was used to determine the between group differences (α <0.05). Results showed TNF-αand NF-κB gene expression in the lung tissue were decreased significantly in swimming training group (Respectively P=0.0001, P=0.001). Since TNF-αand NF-κB are responsible for beginning the cascade of proinflammatory cytokines and chemokines that induce inflammatory responses, reduction in the gene expression of this factors might reduce the risk of pulmonary disorders.
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Main Subjects
- Lawrence T. The nuclear factor NF-κB pathway in inflammation. Cold Spring Harb Perspect Biol. 2009;1(6): a001651.
- Siti HN, Kamisah Y, Kamsiah J. The role of oxidative stress, antioxidants and vascular inflammation in cardiovascular disease (a review). Vascul Pharmacol. 2015;71:40-56.
- Cantin AM, Hartl D, Konstan MW, Chmiel JF. Inflammation in cystic fibrosis lung disease: Pathogenesis and therapy. J Cyst Fibros. 2015;14(4):419-30.
- King PT. Inflammation in chronic obstructive pulmonary disease and its role in cardiovascular disease and lung cancer. Clin Transl Med. 2015; 4(1):1.
- Olivieri D, Scoditti E. Impact of environmental factors on lung defences. Eur Respir Rev. 2005;14(95):51-6.
- Samadi A. Exercise preconditioning and neuroprotection: A review of mechanisms. Shefaye Khatam. 2015;3(1):115-30. (In Persian).
- Alcorn JF, Ckless K, Brown AL, Guala AS, Kolls JK, Poynter ME, et al. Strain-dependent activation of NF-κB in the airway epithelium and its role in allergic airway inflammation. Am J Physiol Lung Cell Mol Physiol. 2010;298(1): 57-66.
- Poynter ME, Cloots R, van Woerkom T, Butnor KJ, Vacek P, Taatjes DJ, et al. NF-κB activation in airways modulates allergic inflammation but not hyperresponsiveness. J Immunol. 2004;173(11):7003-9.
- Waters JP, Pober JS, Bradley JR. Tumour necrosis factor and cancer. J Pathol. 2013;230(3):241-8.
- Durham WJ, Li YP, Gerken E, Farid M, Arbogast S, Wolfe RR, et al. Fatiguing exercise reduces DNA binding activity of NF-κB in skeletal muscle nuclei. J Appl Physiol. 2004;97(5):1740-5.
- Morgan MJ, Liu ZG. Crosstalk of reactive oxygen species and NF-κB signaling. Cell Res. 2011;21(1):103-15.
- Kratsovnik E, Bromberg Y, Sperling O, Zoref-Shani E. Oxidative stress activates transcription factor NF-κB-mediated protective signaling in primary rat neuronal cultures. J Mol Neurosci. 2005;26(1):27-32.
- Rahman I, Gilmour PS, Jimenez LA, MacNee W. Oxidative stress and TNF-a induce histone acetylation and NF-кB/AP-1 activation in alveolar epithelial cells: Potential mechanism in gene transcription in lung inflammation. Mol Cell Biochem. 2002;234-235(1-2):239-48.
- Wang W, Ge N, Ma A, Liu M, Liang H. Protective effect of Aplysin on IKK/NF-kB signaling pathway and related cytokines in rats exposed to ethanol. FASEB J. 2015;29(1 Supplement):755-18.
- Pak-Rad B, Agha Alinejad H, Zamani A, Fashi M, Rezaei Seraji B, Rajabi Z. Study of effect of aerobic exercise in particulate air pollution TLR4 and IL-1β genes expression in male wistar rats’ heart tissue. Exercise Physiology. 2016; 8(31):77-92. (In Persian).
- Azimian E, Ranjbar R, Shakerian S, Habibi A, Ghafourian M. Comparison of an acute bout of combined exercise training in different intensities on tumor necrosis factor- α (TNFα) and interlokin-6 (IL-6) in active men. Exercise Physiology. 2015;7(28):87-102. (In Persian).
- Sanchavanakit N, Saengtong W, Manokawinchoke J, Pavasant P. TNF-α stimulates MMP-3 production via PGE 2 signalling through the NF-kB and p38 MAPK pathway in a murine cementoblast cell line. Arch Oral Biol. 2015;60(7):1066-74.
- Jeong HJ, Koo HN, Na HJ, Kim MS, Hong SH, Eom JW, et al. Inhibition of TNF-α and IL-6 production by aucubin through blockade of NF-κB activation in RBL-2H3 mast cells. Cytokine. 2002;18(5):252-9.
- Cai Z, Tchou-Wong KM, Rom WN. NF-kappaB in lung tumorigenesis. Cancers (Basel). 2011;3(4):4258-68.
- Ricciardolo FL, Petecchia L, Sorbello V, Di Stefano A, Usai C, Massaglia GM, et al. Bradykinin B2 receptor expression in the bronchial mucosa of allergic asthmatics: The role of NF‐kB. ClinExp Allergy. 2016;46(3):428-38.
- Matera MG, Calzetta L, Cazzola M. TNF-α inhibitors in asthma and COPD: We must not throw the baby out with the bath water. Pulm Pharmacol Ther. 2010;23(2):121-28.
- Mukhopadhyay S, Hoidal JR, Mukherjee TK. Role of TNFα in pulmonary pathophysiology. Respir Res. 2006;11(7):125.
- Kalesi M, Gaeini A, Shabkhiz F, Samadi A, Tork F. The effect of a period of discontinuous endurance exercise on ICAM-1 and lipid profile of non-athletic male students. Jsums Med sab.2011;18(3):198-205. (In Persian).
- Vieira RD, Toledo AC, Silva LB, Almeida FM, Damaceno-Rodrigues NR, Caldini EG, et al. Anti-inflammatory effects of aerobic exercise in mice exposed to air pollution. Med Sci Sports Exerc. 2012;44(7):1227-34.
- Yu YB, Liao YW, Su KH, Chang TM, Shyue SK, Kou YR, et al. Prior exercise training alleviates the lung inflammation induced by subsequent exposure to environmental cigarette smoke. ActaPhysiol (Oxf). 2012;205(4):532-40.
- Fashi M, Agha-Alinejad H, Mahabadi HA, Rezaei B, Pakrad BB, Rezaei S. The effect of aerobic exercise in ambient particulate matter on lung tissue inflammation and lung cancer. Iran J Cancer Prev. 2015;8(3): 2333.
- Dempsey JA, Romer L, Rodman J, Miller J, Smith C. Consequences of exercise-induced respiratory muscle work. Respir Physiol Neurobiol. 2006. 28;151(2-3): 242-50.
- Enright SJ, Unnithan VB, Heward C, Withnall L, Davies DH. Effect of high-intensity inspiratory muscle training on lung volumes, diaphragm thickness, and exercise capacity in subjects who are healthy. Phys Ther. 2006;86(3):345-54.
- Mirdar Sh , Arab A, Hedayati M, Hajizade A. The effect of pregnant rat swimming on hypoxia-inducible factor-1α levels of neonatal lung. TUMS. 2012.1;69(12): 754-60. (In Persian).
- Abbass GN, NavabehZare K, Saleh Rahmati A, Rozita F. Visfatin gene responses to 8 weeks of treadmill running with or without pistachio atlantica liquid extraction in female rat tissues.FNS. 2012;28: 1144 –9.
- Normando VM, Mazzoli-Rocha F, Moreira DK, Barcellos BC, Picanço-Diniz DW, Zin WA. Regular exercise training attenuates pulmonary inflammatory responses to inhaled alumina refinery dust in mice. Respir Physiol Neurobiol. 2013.1;186(1): 53-60.
- Matsui E. Aerobic exercise attenuates airway inflammatory responses in a mouse model of atopic asthma. J Immunol. 2004.1;172(7):4520-6.
- Rabinovich RA, Figueras M, Ardite E, Carbó N, Troosters T, Filella X, et al. Increased tumour necrosis factor‐α plasma levels during moderate-intensity exercise in COPD patients. EurRespir J. 2003;21(5):789-94.
- Jing H, Lee S. NF-{kappa} B in cellular senescence and cancer treatment. Mol Cells. 2014;37(3):189-95.
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