Reduction of Tumor Necrosis Factor Alpha and Nuclear Factor- Kappa B Gene Expression in Lung Tissue of Rats After a Period of Swimming Training

Document Type : Research Paper

Authors

1 Ph.D. in Sport Physiology, University of Mazandaran

2 Professor Sport Physiology, University of Mazandaran

3 Assistant Professor of Sport Physiology, Shahed University, Tehran

Abstract

Considering the important role of inflammation in pulmonary disorders the purpose of this study was to investigate the effect of a period of swimming training on Tumor necrosis factor alpha (TNF-α)and Nuclear factor- kappa B (NF-κB) gene expression in lung tissue of wistar rats. Twenty wistar rats of five weeks ages with the weight range of 102±23 g randomly divided into control (n=10) and swimming training groups (n=10). The training protocol included three months of swimming (5 times per week) which begun from 25 minutes in the first week (with the water flow intensity of 4 liters per minute) and reached to 60 minutes (with the water flow intensity of 10 liters per minute) at the final week. Quantitative Real-Time PCR used for detecting and quantifying expression profiles of TNF-αand NF-κB genes in the lung tissue of rats. Kolmogorov-Smirnov test was used to test the normality of data distribution and Independent t test was used to determine the between group differences (α <0.05). Results showed TNF-αand NF-κB gene expression in the lung tissue were decreased significantly in swimming training group (Respectively P=0.0001, P=0.001). Since TNF-αand NF-κB are responsible for beginning the cascade of proinflammatory cytokines and chemokines that induce inflammatory responses, reduction in the gene expression of this factors might reduce the risk of pulmonary disorders.

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Main Subjects

References

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Sport Physiology
Volume 10, Issue 37
Spring 2018
April 2018
Pages 153-166

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History

  • Receive Date: 08 October 2016
  • Revise Date: 03 January 2017
  • Accept Date: 04 January 2017

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