Sport Physiology

Sport Physiology

Effect of Single Bout of Continuous Exercise and High Intensity Interval Exercise on HIF-1α and TNF‌-α Levels in Adipose Tissue of Obese Male Wistar Rats

Document Type : Research Paper

Authors
Maryam Nourshahi 1
Nazanin Aboozari 2
Fariba Khodagholi 3
1 Associated Professor of Sport Physiology, Shahid Beheshti University
2 Ph.D. Student in Sport Physiology, Shahid Beheshti University
3 Associated Professor at Neuroscience Research Center, Shahid Beheshti University of Medical Sciences
10.22089/spj.2018.2303.1311
Abstract
The purpose of this study was to determine the effect of single bout of continuous exercise and high intensity interval exercise on Hypoxia Inducible Factor-1alph (HIF-1α) and Tumor Necrosis Factor-alph (TNFα) levels in adipose tissue of obese male Wistar rats. Subject was 30 obese male Wistar rat that intake high fat diet for 8 weeks. Subject done an exhaustive test to assess VO2max for determine the exercise intensity then divided into three groups: Control, continuous and High Intensity Interval Exercise (HIIE). The HIIE protocol was five 2min sets of high intensity exercise (100% vVO2max) which separated whit four sets of low intensity exercise (30% vVO2max). Continues group intensity was 65% vVO2max and the running time measured by interval sets distance in HIE group. Western blotting analysis use for determine protein levels in visceral and subcutaneous adipose tissue and data analysis was performed with SPSS software. Based on data HIF-1α level reduce significantly 33.5% and 45% in continues and HIIE group respectively (P<0.001). Also, TNF-α level was reduce significantly in both experimental group compare to control and reduction significantly lower in continues group compare to HIIE group(P<0.001). In conclusion exercise specially HIIE that seems to be harmful for obese people, probably does not induce increase in hypoxia an inflammation in adipose tissue and reject the possibility of systemic inflammation and subsequently problem after a bout of intense exercise.
Keywords
Continuous Aerobic
Interval
Hypoxia Inducible Factor-1α
Tumor Necrosis Factor-α
obesity
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Sport Physiology
Volume 9, Issue 36 - Serial Number 36
Winter 2018
Winter 2018
Pages 73-88

  • Receive Date 16 March 2016
  • Revise Date 18 June 2016
  • Accept Date 18 July 2016