The Effect of Pretreatment with Interval Training and Vitamin D on IL-10، BDNF and TNFα in Brain Rat Model of Experimental Autoimmune Encephalomyelitis (EAE)

Document Type : Research Paper

Authors

1 Ph.D. in Sport Physiology, University of Mazandaran

2 Associate Professor of Sport Physiology, University of Mazandaran

3 Ph.D. Student in Sport Physiology, University of Mazandaran

Abstract

The present study aimed at investigating the pre-treatment effect of interval exercises and vitamin D supplementation on BDNF and TNFα of the brain in the rat model of experimental autoimmune encephalomyelitis (EAE). Thirty Lewis rats (six-week old) were assigned to six groups: healthy control, EAE control, EAE control D, healthy interval D, interval EAE D, and sham. Animals were subjected to 6 weeks of interval running training, 5 days in each week, which were performed 10 reps of 1-minute in each. In addition, work to rest ratio of 1: 2 and the running overall time of 30 minutes was considered. After completing the training period, the rats were immunized with guinea pig spinal cord and complete adjuvant. The disease progress and the clinical changes in rats were evaluated over 2 weeks after inducing the disease model. The results showed that there was a significant increase in IL-10 (P=0.003) and a significant decrease in TNF-α (P=0.001) in Lewis female rats with experimental autoimmune encephalomyelitis, but there was no significant change in BDNF (P=0.62). Furthermore, the clinical symptoms in trained rats were delayed. According to the results of this study, it seems that six weeks of interval training along with vitamin D supplementation can not only postpone the onset of clinical symptoms but also increase the anti-inflammatory and inflammation indices in the brain of experimental autoimmune encephalomyelitis models, and thus could help neuroprotection.

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References

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Sport Physiology
Volume 9, Issue 36 - Serial Number 36
Winter 2018
January 2018
Pages 129-142

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History

  • Receive Date: 17 June 2017
  • Revise Date: 10 October 2017
  • Accept Date: 23 October 2017

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