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Effect of 12 Weeks Moderate Intensity Resistance Training and Green Tea Extract on Cardiac Caspase-3 Expression and Telomerase Enzyme Content in Aged Male Rats

Document Type : Research Paper

Authors

1 Ph.D. Student of Exercise Physiology, Urmia University

2 Assistant Professor of Exercise Physiology, Urmia University

3 Associate Professor of Physiology, Urmia University of Medical Sciences

Abstract

The prevalence, incidence, and morbidity of cardiac diseases increase with aging. Cardiomyocyte apoptosis contributes to the pathogenesis of heart failure. The aim of this study was to evaluate the effect of 12 weeks resistance training and green tea extract on cardiac caspase 3 expressions and telomerase enzyme content in aged male rats. In this study, 20 aged male rats were randomly divided into four groups: control, exercise, supplement and exercise plus supplement. Exercise groups performed resistance exercise for 12 weeks and five days per week; meantime, the supplementary groups consumed green tea extract. Immunohistochemistry method was used to measure the expression of caspase 3 and sandwich ELISA method was used to measure the telomerase content. Data was analyzed by ANOVA and Tukey's post hoc test .The results show that caspase 3 expressions in exercise (P=0.011), supplement (P=0.001) and exercise plus supplement (P=0.000) groups was significantly lower than control group. caspase 3 expressions in exercise plus supplement group significantly lower than exercise group (P=0.004) and supplement group (P=0.031). Telomerase content in exercise (P=0.043), supplement (P=0.015) and exercise plus supplement (P=0.005) groups was significantly higher than control group. According to the results of this of this study, the combination of resistance training and green tea extract can prevent cell aging in the cardiac tissue of elderly rats with decreasing expression of Caspase-3 and increased content of telomerase enzymes in the cardiac tissue.

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References
  1. Werner C, Hanhoun M, Widmann T, Kazakov A, Semenov A, Pöss J, et al. Effects of physical exercise on myocardial telomere-regulating proteins, survival pathways, and apoptosis. J Am Coll Cardiol. 2008;52(6):470-82.
  2. Müezzinler A, Zaineddin AK, Brenner H. A systematic review of leukocyte telomere length and age in adults. Ageing Res Rev. 2013;12(2):509-19.
  3. Zalli A, Carvalho LA, Lin J, Hamer M, Erusalimsky JD, Blackburn EH, et al. Shorter telomeres with high telomerase activity are associated with raised allostatic load and impoverished psychosocial resources. Proc Natl Acad Sci U S A. 2014;111(12):4519-24.
  4. Chilton W, O’Brien B, Charchar F. Telomeres, aging and exercise: Guilty by association? Int J Mol Sci. 2017;18(12):25-73.
  5. Haendeler J, Hoffmann J, Brandes RP, Zeiher AM, Dimmeler S. Hydrogen peroxide triggers nuclear export of telomerase reverse transcriptase via Src kinase family-dependent phosphorylation of tyrosine 707. Mol Cell Biol. 2003;23(13):4598-610.
  6. Nazem MR, Emami A, Movafagh A, Pejhan N, Hedayati M. Significance of telomere and telomerase biology and their relationship with aging process. Genet 3rd Millennium. 2012;10(2):2736-45. (In Persian).
  7. Torella D, Rota M, Nurzynska D, Musso E, Monsen A, Shiraishi I, et al. Cardiac stem cell and myocyte aging, heart failure, and insulin-like growth factor-1 overexpression. Circ Res. 2004;94(4):514-24.
  8. Shih H, Lee B, Lee RJ, Boyle AJ. The aging heart and post-infarction left ventricular remodeling. J Am Coll Cardiol. 2011;57(1):9-17.
  9. Ashe PC, Berry MD. Apoptotic signaling cascades. P Prog Neuropsychopharmacol Biol Psychiatry. 2003;27(2):199-214.
  10. Shi J, Zhao Y, Wang Y, Gao W, Ding J, Li P, et al. Nature. 2014; 514(7521):     187-92.
  11. Bowles DK, Laughlin MH. Mechanism of beneficial effects of physical activity on atherosclerosis and coronary heart disease. J Appl Physiol (1985). 2011;111(1):  308-10.
  12. Siagian M, Lousiana M, Santoso DI, Endardjo S. Effects of anaerobic exercise and detraining on the caspase-3 expression of rat ventricular cardiomyocyte. Med J Indones. 2015;24(2):84-90.
  13. Hsieh S-F, Hu G-C, Chuang Y-C, Chen C-Y, Hu Y-N. The effects and safety of exercise training in subjects with chronic heart failure—do elder subjects gain similar benefits? Int J Gerontol. 2010;4(4):165-70.
  14. Jafari A, Pourrazi H, Nikookheslat S, Baradaran B. Effect of exercise training on bcl-2 and bax gene expression in the rat heart. Gene, Cell, Tissue. 2015;2(4):       328-33.
  15. Ko I-G, Kim S-E, Kim C-J, Jee Y-S. Treadmill exercise alleviates aging-induced apoptosis in rat cardiac myocytes. Int J Gerontol. 2013;7(3):152-7.
  16. Kwak H-B, Song W, Lawler JM. Exercise training attenuates age-induced elevation in Bax/Bcl-2 ratio, apoptosis, and remodeling in the rat heart. FASEB J. 2006;20(6):791-3.
  17. Barauna VG, Rosa KT, Irigoyen MC, De Oliveira EM. Effects of resistance training on ventricular function and hypertrophy in a rat model. Clin Med Res. 2007;5(2):114-20.
  18. Yousef D, Farhad GS, Ghiassie R, Saeid S. Effect of resistance exercise on cardiac apoptosis following of ischemic/reperfusion. J Anim Vet Adv. 2011;10(19):2561-6.
  19. Berent R, von Duvillard SP, Crouse SF, Sinzinger H, Green JS, Schmid P. Resistance training dose response in combined endurance-resistance training in patients with cardiovascular disease: a randomized trial. Arch Phys Med Rehabil. 2011;92(10):1527-33.
  20. Bjarnason-Wehrens B, Mayer-Berger W, Meister E, Baum K, Hambrecht R, Gielen S. Recommendations for resistance exercise in cardiac rehabilitation. Recommendations of the German federation for cardiovascular prevention and rehabilitation. Eur J Cardiovasc Prev Rehabil. 2004;11(4):352-61.
  21. Lakhani SA, Masud A, Kuida K, Porter GA, Booth CJ, Mehal WZ, et al. Caspases 3 and 7: Key mediators of mitochondrial events of apoptosis. Science. 2006;311(5762):847-51.
  22. Chu C, Deng J, Man Y, Qu Y. Green Tea Extracts Epigallocatechin-3-gallate for Different Treatments. Biomed Res Int. 2017; 2017: Article ID 5615647, 9 pages.
  23. Sheng R, Gu ZL, Xie ML, Zhou WX, Guo CY. Epigallocatechin gallate protects H9c2 cardiomyoblasts against hydrogen dioxides- induced apoptosis and telomere attrition. Eur J Pharmacol. 2010;641(2-3):199-206.
  24. Sheng R, Gu ZL, Xie ML, Zhou WX, Guo CY. EGCG inhibits proliferation of cardiac fibroblasts in rats with cardiac hypertrophy. Planta Med. 2009;75(2):113-20.
  25. Sheng R, Gu ZL, Xie ML. Epigallocatechin gallate, the major component of polyphenols in green tea, inhibits telomere attrition mediated cardiomyocyte apoptosis in cardiac hypertrophy. Int J Cardiol. 2013;162(3):199-209.
  26. Boren J, Brindle K. Apoptosis-induced mitochondrial dysfunction causes cytoplasmic lipid droplet formation. Cell Death Differ. 2012;19(9):1561-70.
  27. Maki KC, Reeves MS, Farmer M, Yasunaga K, Matsuo N, Katsuragi Y, et al. Green tea catechin consumption enhances exercise-induced abdominal fat loss in overweight and obese adults. J Nutr. 2008;139(2):264-70.
  28. Murase T, Haramizu S, Shimotoyodome A, Tokimitsu I, Hase T. Green tea extract improves running endurance in mice by stimulating lipid utilization during exercise. Am J Physiol Regul Integr Comp Physiol. 2006;290(6): 1550-6.
  29. Nagao T, Hase T, Tokimitsu I. A green tea extract high in catechins reduces body fat and cardiovascular risks in humans. Obesity. 2007;15(6):1473-83.
  30. Thielecke F, Rahn G, Böhnke J, Adams F, Birkenfeld AL, Jordan J, et al. Epigallocatechin-3-gallate and postprandial fat oxidation in overweight/obese male volunteers: a pilot study. Eur J Clin Nutr. 2010;64(7):704-13.
  31. Venables MC, Hulston CJ, Cox HR, Jeukendrup AE. Green tea extract ingestion, fat oxidation, and glucose tolerance in healthy humans. Am J Clin Nutr. 2008;87(3):778-84.
  32. Call JA, Voelker KA, Wolff AV, McMillan RP, Evans NP, Hulver MW, et al. Endurance capacity in maturing mdx mice is markedly enhanced by combined voluntary wheel running and green tea extract. J Appl Physiol (1985). 2008;105(3):923-32.
  33. Richards JC, Lonac MC, Johnson TK, Schweder MM, Bell C. Epigallocatechin-3-gallate increases maximal oxygen uptake in adult humans. Med Sci Sports Exerc. 2010;42(4):739-44.
  34. Cardoso GA, Salgado JM, Cesar MdC, Donado-Pestana CM. The effects of green tea consumption and resistance training on body composition and resting metabolic rate in overweight or obese women. J Med Food. 2013;16(2):120-7.
  35. Gonçalves L, de Souza RR, Maifrino LBM, Caperuto ÉC, Carbone PO, Rodrigues B, et al. Resistance exercise and testosterone treatment alters the proportion of numerical density of capillaries of the left ventricle of aging Wistar rats. Aging Male. 2014;17(4):243-7.
  36. Nucci RAB, Teodoro ACdS, Krause Neto W, Silva WdA, de Souza RR, Anaruma CA, et al. Effects of resistance training on liver structure and function of aged rats. Aging Male. 2018; 21(1):60-64.
  37. Souza RR, de França E, Madureira D, Pontes CC, Santana JO, Caperuto EC. Resistance training improves aortic structure in Wistar rats. Braz J Phys Ther. 2017;21(4):244-50..
  38. Gad SB, Zaghloul DM. Beneficial effects of green tea extract on liver and kidney functions, ultrastructure, lipid profile and hematological parameters in aged male rats. Global Vet. 2013;11(2):191-205.
  39. Keele JA, Stayton MM. Immunohistochemistry in the heart: A protocol manual 2008 [cited 2016 Aug 12]. Available from: http://www.uwyo.edu/molecbio/faculty-and-staff/mark-stayton/_files/ihc-heart-protocols.pdf.
  40. Akbari H, Maleki MJ, Ravasi AA, kordi MR, Dizagi A, Miri M, et al. The effect of an endurance training period with cellular Anti-aging purpose on telomerase enzyme activity in cardiac tissue and peripheral blood lymphocytes in male rats. J Med Counc I.R. Iran. 2014;31(4):389-96. (In Persian).
  41. Okutucu B, Dınçer A, Habib Ö, Zıhnıoglu F. Comparison of five methods for determination of total plasma protein concentration. J Biochem Biophys Meth. 2007;70(5):709-11.
  42. Dimauro I, Scalabrin M, Fantini C, Grazioli E, Valls MRB, Mercatelli N, et al. Resistance training and redox homeostasis: Correlation with age-associated genomic changes. Redox Biol. 2016;10:34-44.
  43. Cherkas LF, Hunkin JL, Kato BS, Richards JB, Gardner JP, Surdulescu GL, et al. The association between physical activity in leisure time and leukocyte telomere length. Arch InternMed. 2008;168(2):154-8.
  44. Savela S, Saijonmaa O, Strandberg TE, Koistinen P, Strandberg AY, Tilvis RS, et al. Physical activity in midlife and telomere length measured in old age. Exp Gerontol. 2013;48(1):81-4.
  45. Rice-Evans C. Implications of the mechanisms of action of tea polyphenols as antioxidants in vitro for chemoprevention in humans. Proc Soc Exp Biol Med. 1999;220(4):262-6.
  46. Arsenis NC, You T, Ogawa EF, Tinsley GM, Zuo L. Physical activity and telomere length: Impact of aging and potential mechanisms of action. Oncotarget. 2017; 8(27):45008-19.
  47. Starr JM, Shiels PG, Harris SE, Pattie A, Pearce MS, Relton CL, et al. Oxidative stress, telomere length and biomarkers of physical aging in a cohort aged 79 years from the 1932 Scottish Mental Survey. Mech Ageing Dev. 2008;129(12):745-51.
  48. Valls MRB, Dimauro I, Brunelli A, Tranchita E, Ciminelli E, Caserotti P, et al. Explosive type of moderate-resistance training induces functional, cardiovascular, and molecular adaptations in the elderly. Age. 2014;36(2):759-72.
  49. Barari A, Bashiri J, Farzanegi P, Fayyaziniya V. The effect of endurance and circuit resistance training on serum superoxide dismutase and heat shock protein 70 levels in inactive college students. J Iran Univ Med Sci. 2015;22(134):9-17. (In Persian).
  50. Ghiasi R, Mohammadi M, Helan JA, Jozani SRJ, Mohammadi S, Ghiasi A, et al. Influence of Two Various Durations of Resistance Exercise on Oxidative Stress in the Male Rat’s Hearts. J Cardiovasc Thorac Res. 2015;7(4):149-53.
  51. Huang C-Y, Lin Y-Y, Hsu C-C, Cheng S-M, Shyu W-C, Ting H, et al. Antiapoptotic effect of exercise training on ovariectomized rat hearts. J Appl Physiol (1985). 2016;121(2):457-65.
  52. Soheily S, Yadegari Hemat Abadi E, Shakeri N. The effect of endurance and resistance training on interleukin-6 and tumor necrosis factor-α in overweight young woman. Journal of Sport Biosciences. 2016;8(2):263-76. (In Persian).
  53. Phillips MD, Flynn MG, McFarlin BK, Stewart LK, Timmerman KL. Resistance training at eight-repetition maximum reduces the inflammatory milieu in elderly women. Med Sci Sports Exerc. 2010;42(2):314-25.
Sport Physiology
Volume 10, Issue 39
Fall 2018
November 2019
Pages 107-126
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History
  • Receive Date: 05 December 2017
  • Revise Date: 12 May 2018
  • Accept Date: 10 June 2018
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