Effect of 12 Weeks Moderate Intensity Resistance Training and Green Tea Extract on Cardiac Caspase-3 Expression and Telomerase Enzyme Content in Aged Male Rats

Document Type : Research Paper

Authors
Mirza Hossein Norouzi Kamareh 1
Mohammad Reza Zolfaghari 2
Firouz Ghaderi Pakdel 3
Javad Tolouei Azar 2
1 Ph.D. Student of Exercise Physiology, Urmia University
2 Assistant Professor of Exercise Physiology, Urmia University
3 Associate Professor of Physiology, Urmia University of Medical Sciences
10.22089/spj.2018.5146.1687
Abstract
The prevalence, incidence, and morbidity of cardiac diseases increase with aging. Cardiomyocyte apoptosis contributes to the pathogenesis of heart failure. The aim of this study was to evaluate the effect of 12 weeks resistance training and green tea extract on cardiac caspase 3 expressions and telomerase enzyme content in aged male rats. In this study, 20 aged male rats were randomly divided into four groups: control, exercise, supplement and exercise plus supplement. Exercise groups performed resistance exercise for 12 weeks and five days per week; meantime, the supplementary groups consumed green tea extract. Immunohistochemistry method was used to measure the expression of caspase 3 and sandwich ELISA method was used to measure the telomerase content. Data was analyzed by ANOVA and Tukey's post hoc test .The results show that caspase 3 expressions in exercise (P=0.011), supplement (P=0.001) and exercise plus supplement (P=0.000) groups was significantly lower than control group. caspase 3 expressions in exercise plus supplement group significantly lower than exercise group (P=0.004) and supplement group (P=0.031). Telomerase content in exercise (P=0.043), supplement (P=0.015) and exercise plus supplement (P=0.005) groups was significantly higher than control group. According to the results of this of this study, the combination of resistance training and green tea extract can prevent cell aging in the cardiac tissue of elderly rats with decreasing expression of Caspase-3 and increased content of telomerase enzymes in the cardiac tissue.
Keywords
Aging
Cardiac Tissue
Resistance Training
Caspase-3
Telomerase Content
Green Tea

Main Subjects

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Sport Physiology
Volume 10, Issue 39
Fall 2018
November 2019
Pages 107-126

  • Receive Date 05 December 2017
  • Revise Date 12 May 2018
  • Accept Date 10 June 2018