Effect of Aerobic Exercise on the Hematological Parameters Modification in Horses Producing Monovalent Snake Antivenin

Document Type : Research Paper

Authors

1 Ph.D. Student in Sport Physiology, Islamic Azad University South Tehran Branch

2 Professor of Human Serum Razi Vaccine and Serum Research Institute, Agricultural Research and Extension Organization, Karaj

3 Associate Professor of Sport Physiology, Islamic Azad University South Tehran Branch

4 Assistant Professor of Sport Physiology, Olum Tahghighat University, Tehran

Abstract
Snake antivenin production is associated with several hematological changes in horses affecting their health. Exercise training, as a non-pharmacological method has an important role in maintaining and improving livestock health. This study is aimed to investigate effect of aerobic exercise on the Hematological Parameters modification in Horses Producing monovalent snake Antivenin. Ten horses of mixed-breed of 8 to 5 years old with an average weight of 300-350 kg in antivenin production cycle were randomly divided into two groups of venom (V) and venom + exercise (TV) . The training protocol included 3 sessions of aerobic training in a week with medium intensity for 22 weeks. Each training session included of aerobic training with 50-60% of maxHR,. Blood samples were collected before and after training period through external jugular vein. Repeated measure method was used to analyze the data. Results showed that red blood cells count, hemoglobin, hematocrit, platelets count, white blood cells count, lymphocytes, and eosinophil decreased while MCH, MCHC, MCV, monocyte, and neutrophils increased significantly within both groups. However, these changes were not significant between the two groups (p≥0.05). Overall, the results of this study indicated that hematological changes in horses seem to be related to the venom and this intensity does not have a significant effect on hematological parameters modification in venom + exercise.

Keywords

Main Subjects

  1. Jamunaa A, Vejayan J, Halijah I, Sharifah SH, Ambu S. Cytotoxicity of Southeast Asian snake venoms. J VENOM ANIM TOXINS. 2012;18(2):150-6.
  2. Mackessy SP. The field of reptile toxinology: Snakes, lizards and their venoms. In: Mackessy SP, editor. Handbook of venoms and toxins of reptiles. Boca Raton. CRC Press; 2009; p. 3-23.
  3. Gutiérrez JM, León G. Snake antivenoms. Technological, clinical and public health issues. Animal Toxins: State of the Art. Ind Biotechnol. 2009 2009;15(3):393-421.
  4. Braud S, Bon C, Wisner A. Snake venom proteins acting on hemostasis. Biochimie. 2000;82(9-10):851-9.
  5. Tizard IR. Innate immunity: the recognition of invaders. Veterinary Immunology: An Introduction. 8th ed. Philadelphia, PA: Saunders. 2008:11-20.
  6. Lisičić D, Đikić D, Benković V, Knežević AH, Oršolić N, Tadić Z. Biochemical and hematological profiles of a wild population of the nose-horned viper Vipera ammodytes (Serpentes: Viperidae) during autumn, with a morphological assessment of blood cells. ZOOL STUD. 2013;52(1): 1-9.
  7. Nicolson IC, Ashby PA, Johnson ND, Versey J, Slater L. Boomslang bite with haemorrhage and activation of complement by the alternate pathway. Clin Exp Immunol. 1974 Feb;16(2):295.
  8. Barraviera B, Peraçoli MT. Soroterapia heteróloga. Barraviera B. Venenos Animais–uma visão integrada. Rio de Janeiro: EPUC. 1994.
  9. Cardoso DF, Mota I. Effect of crotalus venom on the humoral and cellular immune response. Toxicon. 1997;35(4):607-12.
  10. Angulo Y, Estrada R, Gutiérrez JM. Clinical and laboratory alterations in horses during immunization with snake venoms for the production of polyvalent (Crotalinae) antivenom. Toxicon. 1997;35(1):81-90.
  11. Chippaux JP, Goyffon M. Venoms, antivenoms and immunotherapy. Toxicon. 1998;36(6):823-46.
  12. Mackinnon L. Exercise and immunology (Current issues in exercise science series). Bristol: Society for Endocrinology; 1992.
  13. Snow DH, Kerr MG, Nimmo MA, Abbott EM. Alterations in blood, sweat, urine and muscle composition during prolonged exercise in the horse. Veterinary Record. 1982;110(16):377-84.
  14. Rose RJ, Hodgson DR. Haematological and plasma biochemical parameters in endurance horses during training. EQUINE VET J. 1982;14(2):144-8.
  15. Vidman MP, Greisheimer EM. Physiology and anatomy. 10th ed. Philadelphia: Lippincott Williams and Wilkins; 2002.
  16. Wilmore JH, Costill DL.Physiology of sport and exercise. 2nd ed. Indiana: Human Kinetics; 2005.
  17. Evans D. Training and fitness in athletic horses: A report for the rural industries research and development corporation. University of Sydne: RIRDC Publication; 2000.
  18. Gibbs PG, Potter GD, Nielsen BD, Householder DD, Moyer W. Scientific principles for conditioning race and performance horses1. ARPAS. 1995;11(4):195-203
  19. Burlikowska K, Boguslawska-Tryk M, Szymeczko R, Piotrowska A. Haematological and biochemical blood parameters in horses used for sport and recreation. JCEA. 2015;16(4):370-82.
  20. Cywińska A, Szarska E, Górecka R, Witkowski L, Hecold M, Bereznowski A, Schollenberger A, Winnicka A. Acute phase protein concentrations after limited distance and long-distance endurance rides in horses. RES VET SCI. 2012 Dec 1;93(3):1402-6.
  21. Netto DP, Chiacchio SB, Bicudo PL, Alfieri AA, Balarim, MRS,Nascimento N. Hematological changes in sheep inoculated with natural and Cobalt60-irradiated Crotalus durissus terrificus venom (Laurenti, 1768). J Venom Anim Toxins Incl. Trop Dis. 2004;10:34-52.
  22. Waghmare AB, Salvi NC, Deopurkar RL, Shenoy PA, Sonpetkar JM. Evaluation of health status of horses immunized with snake venom and montanide adjuvants, IMS 3012 (nanoparticle), ISA 206 and ISA 35 (emulsion based) during polyvalent snake antivenom production: Hematological and biochemical assessment. Toxicon. 2014;82:83-92.
  23. Nieman DC. Immune response to heavy exertion. J APPL PHYSIOL. 1997;82(5):1385-94.
  24. Waghmare AB, Salvi NC, Deopurkar RL, Shenoy PA, Sonpetkar JM. Evaluation of health status of horses immunized with snake venom and montanide adjuvants, IMS 3012 (nanoparticle), ISA 206 and ISA 35 (emulsion based) during polyvalent snake antivenom production: Hematological and biochemical assessment. Toxicon. 2018;82:83-92
  25. Padalino B, Rubino G, Centoducati P, Petazzi F. Training versus overtraining: evaluation of two protocols. J EQUINE VET SCI. 2007;27(1):28-31.
  26. Emam SJ, Nikzamir A. Evaluation of hematological and biochemical parameters in snakebite patients referred to Razi Hospital, Ahwaz, Iran. Pak J Med Sci. 2008;24:712-8.
  27. Lucas de Oliveira PC, Sakate M, Madruga RA, Barbosa NP. Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venom. J VENOM ANIM TOXINS. 2007;13(3):576-97.
  28. Clarkson GT. Haematology and serum iron in the racehorse. J Vet Record. 1986;118:77–84.
  29. Zech D, Rana S, Büchler MW, Zöller M. Tumor-exosomes and leukocyte activation: An ambivalent crosstalk. JCCS. 2012;10(1):1-17.
  30. Piccione G, Casella S, Giannetto C, Messina V, Monteverde V, Caola G, Guttadauro S. Haematological and haematochemical responses to training and competition in standardbred horses. Comp Clin Pathol. 2010;19(1):95-101.
  31. Cywińska A, Szarska E, Górecka R, Witkowski L, Hecold M, Acute phase protein concentrations after limited distance and long distance endurance rides in horses. RES VET SCI. 2012;93(3):1402-6.
  32. Lucas de Oliveira PC, Sakate M, Madruga RA, Barbosa NP. Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venom. J EQUINE VET SCI.2007;13(3):576-97.
  33. Yamileth A, Ricardo E. Clinical and laboratory alterations in horses during immunization with snake venoms for the production of polyvalent (Crotalinae) mantivenom. J Toxicon. 1997;35(1):81-90
  34. Krumrych W, Gołda R, Gołyński M, Markiewicz H, Buzała M. Effect of physical exercise on cortisol concentration and neutrophil oxygen metabolism in peripheral blood of horses. Ital J Anim Sci. 2018;18(1):53-68.
  35. Jain NC, Jain AH. Essentials of veterinary hematology. Philadelphia: Lea & Febiger; 1993.
  36. Satué K, Hernández A, Muñoz A. Physiological factors in the interpretation of equine hematological profile. InHematology-science and practice 2012 Mar 2. IntechOpen.
  37. Schumacher YO, Grathwohl D, Barturen JM, Wollenweber M, Heinrich L, Schmid A, et al. Haemoglobin, haematocrit and red blood cell indices in elite cyclists: Are the control values for blood testing valid? INT J SPORTS MED. 2000;21(05):380-5.
  38. Vina J, Sanchis‐Gomar F, Martinez‐Bello V, Gomez‐Cabrera MC. Exercise acts as a drug: The pharmacological benefits of exercise. Br J Pharmacol. 2012;167(1):1-2.
Sport Physiology
Volume 10, Issue 40
Winter 2019
January 2019
Pages 99-116

  • Receive Date 17 August 2018
  • Revise Date 08 December 2018
  • Accept Date 16 December 2018