Document Type : Research Paper


1 Ph.D. Candidate in Exercise Physiology, Tarbiat Modares University

2 Professor of Exercise Physiology, Tarbiat Modares University

3 Professor of Exercise Physiology, Kharazmi University

4 Physiatrist, Associate Professor, Aja University of Medical Science

5 Assistant Professor of Exercise Physiology, Razi University of karmanshah


The aim of the present study was to investigate the effect of upper limb exhaustive activity on corticospinal excitability and motoneuron responsiveness of lower limb. Ten active males (Age 28.1 ± 2.99yr, Height 1.77 ± 4.1 cm, Weight 75.7 ± 4.98) volunteered to participate in this study and visited the laboratory on three different occasions. After measuring anthropometric features and familiarization with the pull up (exhaustive task) and magnetic and electrical stimulations in the session one, in the second and third visits, motor evoked potential (MEP), cervicomedullary motor evoked potential (CMEP) and maximum direct motor response (Mmax) were assessed immediately, 10 and 20 minutes after the termination of the exhaustive protocol ( perform pull ups until exhaustion,7 sets with 1min rest intervals) ,by the use of magnetic stimulation over the motor cortex and spinal routes and electrical stimulation of peripheral nerve, respectively. The results showed that MEP in the Tibialis Anterior muscle (remote muscle) significantly decreased immediately (P₌0.001) and 10 minutes (P₌0.001) after performing the fatiguing task compared to the resting values. Also, no significant changes in CMEP and Mmax were observed in Tibialis Anterior muscle at any time points relative to the baseline (P= 0.5, P= 0.07). Based on the data obtained in this study, it looks that supraspinal centers account for the spreading of fatigue from the upper limb to the lower limb. Besides, such a supraspinal fatigue needs a considerable time to be recovered.


Main Subjects

  1. Gandevia SC, Allen GM, Butler JE, Taylor JL. Supraspinal factors in human muscle fatigue: Evidence for suboptimal output from the motor cortex. J physiol. 1996;490(Pt 2):529-36.
  2. Gandevia SC. Spinal and supraspinal factors in human muscle fatigue. Physiol rev. 2001;81(4):1725-89.
  3. McNeil CJ, Butler JE, Taylor JL, Gandevia SC. Testing the excitability of human motoneurons. Frontiers in hum neurosci. 2013;7:152.
  4. McNeil CJ, Martin PG, Gandevia SC, Taylor JL. Long-interval intracortical inhibition in a human hand muscle. Exp brain res. 2011;209(2):287-97.
  5. Goodall S, Howatson G, Romer L, Ross E. Transcranial magnetic stimulation in sport science: a commentary. Eur J sport sci. 2014;14 Suppl 1:332-40.
  6. Reis J, Swayne OB, Vandermeeren Y, Camus M, Dimyan MA, Harris-Love M, et al. Contribution of transcranial magnetic stimulation to the understanding of cortical mechanisms involved in motor control. J physiol. 2008;586(2):325-51.
  7. Tergau F, Geese R, Bauer A, Baur S, Paulus W, Reimers CD. Motor cortex fatigue in sports measured by transcranial magnetic double stimulation. Med sci sports exerc. 2000;32(11):1942-8.
  8. Taylor JL, Gandevia SC. Transcranial magnetic stimulation and human muscle fatigue. Muscle nerve. 2001;24(1):18-29.
  9. Verin E, Ross E, Demoule A, Hopkinson N, Nickol A, Fauroux B, et al. Effects of exhaustive incremental treadmill exercise on diaphragm and quadriceps motor potentials evoked by transcranial magnetic stimulation. J appl physiol. 2004;96(1):253-9.
  10. Taylor JL, Gandevia SC. A comparison of central aspects of fatigue in submaximal and maximal voluntary contractions. J appl physiol. 2008;104(2):542-50.
  11. McNeil CJ, Giesebrecht S, Gandevia SC, Taylor JL. Behaviour of the motoneurone pool in a fatiguing submaximal contraction. J physiol .2011;589(Pt 14):3533-44.
  12. Fulton RC, Strutton PH, McGregor AH, Davey NJ. Fatigue-induced change in corticospinal drive to back muscles in elite rowers. Exp physiol. 2002;87(5):593-600.
  13. Goodall S, Gonzalez-Alonso J, Ali L, Ross EZ, Romer LM. Supraspinal fatigue after normoxic and hypoxic exercise in humans. J physiol. 2012;590(11):2767-82.
  14. Sidhu SK, Bentley DJ, Carroll TJ. Locomotor exercise induces long-lasting impairments in the capacity of the human motor cortex to voluntarily activate knee extensor muscles. J appl physiol. 2009;106(2):556-65.
  15. Ross EZ, Gregson W, Williams K, Robertson C, George K. Muscle contractile function and neural control after repetitive endurance cycling. Med sci sports exerc. 2010;42(1):206-12.
  16. Hoffman BW, Oya T, Carroll TJ, Cresswell AG. Increases in corticospinal responsiveness during a sustained submaximal plantar flexion. J appl physiol. 2009; 107(1):112-20.
  17. Baumer T, Munchau A, Weiller C, Liepert J. Fatigue suppresses ipsilateral intracortical facilitation. Exp brain res. 2002;146(4):467-73.
  18. Lee M, Hinder MR, Gandevia SC, Carroll TJ. The ipsilateral motor cortex contributes to cross-limb transfer of performance gains after ballistic motor practice. J physiol. 2010;588(Pt 1):201-12.
  19. Lee M, Carroll TJ. Cross education: possible mechanisms for the contralateral effects of unilateral resistance training. Sports med. 2007;37(1):1-14.
  20. Post M, Bayrak S, Kernell D, Zijdewind I. Contralateral muscle activity and fatigue in the human first dorsal interosseous muscle. J appl physiol. 2008;105(1):70-82.
  21. Rattey J, Martin PG, Kay D, Cannon J, Marino FE. Contralateral muscle fatigue in human quadriceps muscle: Evidence for a centrally mediated fatigue response and cross-over effect. Eur J physiol. 2006;452(2):199-207.
  22. Takahashi K, Maruyama A, Maeda M, Etoh S, Hirakoba K, Kawahira K, et al. Unilateral grip fatigue reduces short interval intracortical inhibition in ipsilateral primary motor cortex. Clin neurophysiol. 2009;120(1):198-203.
  23. Salehi A, Rahmani Nia B, Mirzaei. Comparison the effect of two types of isotonic and isometric resistance training on untrained leg strength and EMG changes in untrained male students. Sport physiology. 2013; 5(18):107-20. (In Persian).
  24. Takahashi K, Maruyama A, Hirakoba K, Maeda M, Etoh S, Kawahira K, et al. Fatiguing intermittent lower limb exercise influences corticospinal and corticocortical excitability in the nonexercised upper limb. Brain stimul. 2011;4(2):90-6.
  25. Kennedy A, Hug F, Sveistrup H, Guevel A. Fatiguing handgrip exercise alters maximal force-generating capacity of plantar-flexors. Eur J appl physiol. 2013;113(3):559-66.
  26. Frigon A, Carroll TJ, Jones KE, Zehr EP, Collins DF. Ankle position and voluntary contraction alter maximal M waves in soleus and tibialis anterior. Muscle nerve. 2007;35(6):756-66.
  27. Aboodarda SJ, Copithorne DB, Power KE, Drinkwater E, Behm DG. Elbow flexor fatigue modulates central excitability of the knee extensors. Appl physiol nutri metab. 2015;40(9):924-30.
  28. McNeil CJ, Giesebrecht S, Khan SI, Gandevia SC, Taylor JL. The reduction in human motoneurone responsiveness during muscle fatigue is not prevented by increased muscle spindle discharge. J physiol. 2011;589(Pt 15):3731-8.
  29. Byblow WD, Coxon JP, Stinear CM, Fleming MK, Williams G, Muller JF, et al. Functional connectivity between secondary and primary motor areas underlying hand-foot coordination. J neurophysiol. 2007;98(1):414-22.
  30. de Kam D, Rijken H, Manintveld T, Nienhuis B, Dietz V, Duysens J. Arm movements can increase leg muscle activity during submaximal recumbent stepping in neurologically intact individuals. J appl physiol. 2013;115(1):34-42.
  31. Ferris DP, Huang HJ, Kao PC. Moving the arms to activate the legs. Exerc sport sci rev. 2006;34(3):113-20.
  32. Huang HJ, Ferris DP. Neural coupling between upper and lower limbs during recumbent stepping. J appl physiol. 2004;97(4):1299-308.
  33. Zehr EP, Balter JE, Ferris DP, Hundza SR, Loadman PM, Stoloff RH. Neural regulation of rhythmic arm and leg movement is conserved across human locomotor tasks. J physiol. 2007;582(Pt 1):209-27.
  34. Zehr EP, Duysens J. Regulation of arm and leg movement during human locomotion. Neuroscientist. 2004;10(4):347-61.
  35. Raux M, Xie H, Similowski T, Koski L. Facilitatory conditioning of the supplementary motor area in humans enhances the corticophrenic responsiveness to transcranial magnetic stimulation. J appl physiol. 2010;108(1):39-46.
  36. Sidhu SK, Weavil JC, Venturelli M, Garten RS, Rossman MJ, Richardson RS, et al. Spinal mu-opioid receptor-sensitive lower limb muscle afferents determine corticospinal responsiveness and promote central fatigue in upper limb muscle. J physiol. 2014;592(22):5011-24.
  37. Halperin I, Chapman DW, Behm DG. Non-local muscle fatigue: effects and possible mechanisms. Eur J appl physiol. 2015;115(10):2031-48.
  38.  Khosravi A, Mirzaei B , Mehrabani J, Rasoulian B. The interaction effects of aerobic training and Saffron extracts consumption on anti-oxidant defense system of heart and brain premotor cortex of young male rats following an acute bout of exhaustive endurance exercise. Sport physiology. 2015;7(25):109-30. (In Persian).
  39. Rasmussen P, Nielsen J, Overgaard M, Krogh-Madsen R, Gjedde A, Secher NH, et al. Reduced muscle activation during exercise related to brain oxygenation and metabolism in humans. J physioly. 2010;588(Pt 11):1985-95.