نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناسی‌ارشد فیزیولوژی ورزشی، دانشگاه پیام نورکرج

2 استادیار فیزیولوژی ورزشی، دانشگاه شهید بهشتی تهران

3 دانشیار فیزیولوژی ورزشی، دانشگاه پیام نور

چکیده

هدف پژوهش حاضر، بررسی تأثیر شش هفته تمرین استقامتی تداومی بر بیان ژن کاسپاز-3 و متالوتیونئین-2 بافت بطن چپ موش‌های صحرایی نر سالمند بود. تعداد 14 سر‌ موش صحرایی     (26-24 ماهه و میانگین وزنی 20 ± 380 گرم)، به‌طور تصادفی به دو گروه کنترل و تمرین تقسیم شدند. گروه تمرین، تمرین استقامتی تداومی روی تردمیل را به‌مدت شش هفته انجام دادند ( 65 تا 70 درصد سرعت بیشینه).بیان ژن‌های کاسپاز-3 و ‌متالوتیونئین- 2 بافت بطن چپ با تکنیک Real time PCR سنجیده شد و پس از کمی‌سازی مقادیر بیان ژن، با استفاده از فرمول 2-∆∆ct به‌وسیلة روش آماری تی مستقل بررسی شد (P < 0.05). نتایج نشان داد که شش هفته تمرین استقامتی ‌تداومی به کاهش غیرمعنادار بیان ژن کاسپاز-3 (P = 0.078) و افزایش غیرمعنا‌دار بیان ژن متالوتیونئین-2 (P = 0.513) منجر ­شد. به‌نظر می‌رسد که انجام تمرین‌های استقامتی تداومی در سالمندان خطر آسیب‌های ناشی از آپوپتوز را القا نمی‌کند و می‌تواند در این جمعیت به‌کار گرفته شود.

کلیدواژه‌ها

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