Sport Physiology

Sport Physiology

The Effect of Endurance Training and High-Fat Diet on PGC-1a/FNDC5/Irisin Pathway in Male C57BL/6 Mice

Document Type : Research Paper

Authors
Fatemeh Kazemi Nasab 1
Mohammad Marandi 2
Kamran Ghaedi 3
Fahimeh Esfarjani 4
Mohammad Hossein Nasr-Esfahani 5
1 Ph.D. Student of Exercise Physiology, University of Isfahan
2 Professor of Exercise Physiology, University of Isfahan
3 Professor of Biology, University of Isfahan
4 Associate Professor of Exercise Physiology, University of Isfahan
5 Professor of Embryology, Royan Institute for Biotechnology
10.22089/spj.2017.4295.1573
Abstract
The purpose of this study was to investigate the effect of endurance training and high fat diet on PGC-1a/FNDC5/Irisin pathway in Gastrocnemius muscle and visceral adipose tissue in male C57BL/6 mice. Male C57BL/6 mice were divided to 4 groups: High fat-Exercise (HF-E), High fat-Sedentary (HF-S), Low fat-Exercise (LF-E) and Low fat-Sedentary (LF-S). Exercise groups received exercise on a motor-driven treadmill at 21m/min, 45 min/day, 5 days/week for 8 weeks. 24 hours after final exercise session, mice were sacrificed and blood samples were taken from the mice to measure plasma irisin. Gastrocnemius skeletal muscle and visceral adipose tissue were excised and frozen in liquid nitrogen for assessment of PGC-1a and FNDC5 mRNA levels. Data was analyzed by a two-way ANOVA at P0.05). The results of this study indicate that high fat diet increases the expression of FNDC5 in the Gastrocnemius muscle tissue. Also, there is a potential correlation between the expression of muscular FNDC5 and body fat mass, which requires further studies to investigate its cellular and molecular mechanisms.
Keywords
High-Fat Diet
Endurance Training
PGC-1a mRNA
FNDC5 mRNA
Irisin
Subjects
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Sport Physiology
Volume 11, Issue 41
Spring 2019
Pages 63-80

  • Receive Date 08 June 2017
  • Revise Date 26 August 2017
  • Accept Date 17 September 2017