Effect of Aerobic Interval Training on Acylated Ghrelin, Peptide Tyrosine Tyrosine and Glucagon-Like Peptide-1 in Healthy Young Men

Document Type : Research Paper

Authors
Ahmad Jafary 1
Elahe Talebi Garakani 2
Marzieh Saghebjoo 3
Rozita Fathi 2
1 Ph.D. Student of Sport Physiology, University of Mazandaran
2 Associate Professor of Sport Physiology, University of Mazandaran
3 Associate Professor of Sport Physiology, University of Birjand
10.22089/spj.2017.4373.1583
Abstract
The aim of this study was to investigate the effect of aerobic interval training on acylated ghrelin, peptide tyrosine tyrosine (PYY) and glucagon-like peptide-1 (GLP-1) in healthy young men. Twenty male students with age 22.26±1.25 years old and BMI 23.2±1.4 kg/m2 were randomly assigned to the training and control groups. The training group completed a high intensity interval aerobic exercise training for 12 weeks. The training program consists of four minutes running periods on treadmill with intensity of 85-95%HRmax followed by three minutes of 65-75% HRmax rest active periods. The levels of plasma acylated ghrelin, PYY3-36 and GLP-1 were measured by ELISA method before and after eight- and 12-weeks trainings. In data analysis, was used the Mann-Whitney U test in order to compare the control and training groups and the Wilcoxon test in order to compare the training periods. The results showed that in the training group, levels of acylated ghrelin significantly (P=0.008) decreased after 12 weeks compared to the eight weeks. However, there were no significant changes in the acylated ghrelin, PYY and GLP-1 levels compared to the control group after eight and 12 weeks. Overall it seems appetite hormones alterations in young active males in response to aerobic interval trainings were not considerable, but a 12 weeks period of aerobic interval training may decrease appetite compared to the same training during eight weeks.
Keywords
Aerobic Interval Training
Appetite
Acylated Ghrelin
Glucagon-Like Peptide-1

Main Subjects

  1. Jill A, Kanaley I, Timothy D, Adam T. Short term aerobic exercise training increases postprandial pancreatic polypeptide but not peptide YY concentrations in obese individuals. Int J Obes (Lond). 2014;38(2): 266–71.
  2. Douglas JA, King J A, McFarlane E. Appetite, appetite hormone and energy intake responses to two consecutive days of aerobic exercise in healthy young men. Appetite. 2015;92:57-65.  
  3. Thackray AE, Deighton K, King JA and Stensel DJ. Exercise, appetite and weight control: Are there differences between men and women? J Nutrients. 2016;8(9):1-18.
  4. Martins C, Kulseng B, King NA, Holst JJ. The effects of exercise-induced weight loss on appetite-related peptides and motivation to eat. J Clin Endocrinol Metab. 2010;95(4):1609-16.
  5. Spreckley E, Murphy KG. The L-cell in nutritional sensing and the regulation of appetite. Front Nutr. 2015;2:23.1-12
  6. Kalra SP, Dube MG, Pu S, Xu B, Horvath TL, Kalra PS. Interacting appetite regulating pathways in the hypothalamic regulation of body weight. Endocr Rev. 1999;20(1):68-100.
  7. Wren AM, Bloom SR. Gut hormones and appetite control. Gastroenterology. Gastroenterology. 2007;132(6):2116-30.
  8. Ueda SY, Miyamoto T, Nakahara H, Shishido T, Usui T, Katsura Y. Effects of exercise training on gut hormone levels after a single bout of exercise in middle-aged Japanese women. Springerplus. 2013;2(1):83. DOI: 10.1186/2193-1801-2-83.
  9. Justine M, Azizan A, Hassan V, Manaf H. Barriers to participation in physical activity and exercise among middle-aged and elderly individuals. Singapore Med J. 2013;54(10):581-6.
  10. Karlsen T, Aamot IL, Haykowsky M, Rognmo Ø. High intensity interval training for maximizing health outcomes. Prog Cardiovasc Dis. 2017;S0033-0620(17):30051-8. DOI: 10.1016/j.pcad.2017.
  11. Aaron Y. Sim Karen E. Wallman, Timothy J, Kym J. Effects of high-intensity intermittent exercise training on appetite regulation. Med Sci Sports Exerc. 2015; 47(11):2441-9.
  12. Martins C, Truby H, Morgan L M. Short-term appetite control in response to a 6-week exercise programme in sedentary volunteers. Br J Nutr. 2007;98(4):834-42.
  13. Rosenkilde M, Reichkendler MH, Auerbach P, Toräng S, Gram AS, Ploug Tet al. Appetite regulation in overweight, sedentary men after different amounts of endurance exercise: A randomized controlled trial. J Appl Physiol. 2013;115(11): 1599 –609.
  14. King JA, Wasse LK, Stensel DJ, Nimmo MA. Exercise and ghrelin. A narrative overview of research. Appetite. 2013;68:83-91.
  15. Guelfi K J, Donges C E, Duffield R. Beneficial effects of 12 weeks of aerobic compared with resistance exercise training on perceived appetite in previously sedentary overweight and obese men. Metabolism. 2013;62(2):235-43.
  16. Hopkins M, Gibbons C, Caudwell P. The adaptive metabolic response to exercise-induced weight loss influences both energy expenditure and energy intake. Eur J Clin Nutr. 2014;68(5):581–6.
  17. Moradi F, Vosoughy V, Heydarzadeh A. Effect of twelve weeks aerobic training on chemerin, active ghrelin, and appetite in sedentary obese men. Iranian Journal of Metabolism and Exercise. 2013;3(2).89-104. (In Persian).
  18. Thomas S, Reading J, Shephard RJ. Revision of the activity readiness questionnaire (PAR-Q). Can J Sport Sci .1992;17(4):338-45.
  19. Jackson AS, Pollock ML. Practical assessment of body composition. Phys Sportsmed. 1985;13(5):76-90.
  20. Heyward VH, Campaign I L. editors. Advanced fitness assessment and exercise prescription and exercise. 6th ed.United of states of America : Human Kinetics. 2010; P. 233-313.
  21. Ebrahimi M, Rahmani Nia F, Damirchi A, Mirzaie B. Effects of aerobic exercise in tensityon energy intake, appetite and energy-regulating hormones in sedentary young men. J Sport Physiol, 2014;5(20).15-28. (In Persian).
  22. Amiri F, Amani R, Khajemogahi N, Rashidkhani B. Comparison of the effects of two Iranian breakfasts (high-carbohydrate vs. high- protein) on cognitive function of 9-11 year-old primary school children. Nutr Science & Food Technology. 2008;3(2):       23-32. (In Persian).
  23. Flint A, Raben J E, Blundell A. Reproducibility, power and validity of visual analogue scales in assessment of appetite sensations in single test meal studies. Int J Obes Relat Metab Disord. 2000;24(1):38-48.
  24. Lunt H, Draper N, Marshall HC, Logan FJ, Hamlin MJ. High intensity interval training in a real world setting: A randomized controlled feasibility study in overweight inactive adults, measuring change in maximal oxygen uptake. PLoS ONE. 2014;13:9(1):83256. DOI:10.1371/journal.pone.0083256.
  25. Schradin C, Pillay N, Kondratyeva A, Yuen CH, Schoepf I, Krackow S. Basal blood glucose concentration in free-living striped mice is influenced by food availability, ambient temperature and social tactic. Biol Lett. 2015; 11(5):20150208.
  26. Berthoud HR. The vagus nerve, food intake and obesity. Regul Pept. 2008;149          (1-3):15-25. 
  27. Leidy H J, Dougherty K A, Frye B R, Duke K M, Williams N I. Twenty-four-hour ghrelin is elevated after calorie restriction and exercise training in non-obese women. Obesity (Silver Spring). 2007;15(2):446-55.
  28. Chebani Y, Marion C, Zizzari P, Chettab K, Pastor M, Korostelev M. et al. Enhanced responsiveness of Ghsr Q343X rats to ghrelin results in enhanced adiposity without increased appetite. Sci Signal. 2016;9(424):ra39.  
  29. Chen HY, Trumbauer ME, Chen AS, Weingarth DT, Adams JR, Shen Zet aL. Orexigenic action of peripheral ghrelin is mediated by neuropeptide Y and agouti-related protein. Endocrinology. 2004;145(6):2607–12.
  30. Jerlhag E, Egecioglu E, Dickson SL, Douhan A, Svensson L, Engel JA. Ghrelin administration into tegmental areas stimulates locomotor activity and increases extracellular concentration of dopamine in the nucleus accumbens. Addiction Biology. 2007;12(1):6–16.
  31. Delzenne N, Blundell J, Brouns F, Cunningham K, De Graaf K, Erkner A. Gastrointestinal targets of appetite regulation in humans. Obes Rev. 2010;11(3):   234-50.
  32. King NA, Caudwell P, Hopkins M, Stubbs J R, Naslund E, Blundell J E. Dual-process action of exercise on appetite control: Increase in orexigenic drive but improvement in meal-induced satiety. Am J Clin Nutr. 2009;90(4):921–7.
  33. Nauck MA, Siemsgluss J, Orskov C, Holst JJ. Release of glucagon-like peptide 1 (GLP-1 [7-36 amide]), gastric inhibitory polypeptide (GIP) and insulin in response to oral glucose after upper and lower intestinal resections. Z Gastroenterol. 1996;34(3):159–66.
  34. Pilichiewicz AN, Chaikomin R, Brennan IM, Wishart JM. Load-dependent effects of duodenal glucose on glycemia, gastrointestinal hormones, antropyloroduodenal motility, and energy intake in healthy men. Am J Physiol Endocrinol Metab . 2007:293(3):743–53.
  35. Devaskar SU, Giddings SJ, Rajakumar PA, Carnaghi LR, Menon RK. Insulin gene expression and insulin synthesis in mammalian neuronal cells. J Biol Chem. 1994;269(11):8445-54.
  36.  Kleinridders A, Ferris HA, Cai W, Kahn CR. Insulin action in brain regulates systemic metabolism and brain function. Diabetes .2014;63(7):2232–43.  
  37. Kalra SP, Dube MG, Pu S, Xu B, Horvath TL, Kalra PS. Interacting appetite regulating pathways in the hypothalamic regulation of body weight. Endocr Rev. 1999;20(1):68-100.
  38. Crabtree DR, Chambers ES, Hardwick RM, Blannin AK. The effects of high-intensity exercise on neural responses to images of food. Am J Clin Nutr. 2014;99(2):258–67.
  39. Sim AY, Wallman KE, Fairchild TJ, and Guelfi KJ. Effects of high-intensity Intermittent exercise training on appetite regulation. Med. Sci. Sports Exerc. 2015;47(11):2441–9.
  40. Naleid AM, Grace MK, Cummings DE, and Levine AS. Ghrelin induces feeding in the mesolimbic reward pathway between the ventral tegmental area and the nucleus accumbens. Peptides. 2005;26(11):2274–9.
Sport Physiology
Volume 11, Issue 41
April 2019
Pages 105-122

  • Receive Date 20 June 2017
  • Revise Date 19 August 2017
  • Accept Date 17 September 2017