Acute Interval Walking with Blood Flow Restriction Does Not Activate MAPK Pathway Signaling in Inactive Men

Document Type : Research Paper

Authors
Mehdi Khoubi 1
Abdolhamid Habibi 2
Mmohsen Ghanbarzadeh 3
Saeed Shakerian 4
Bahman Mirzaie 5
1 Ph.D. Student in Exercise Physiology, Shahid Chamran University of Ahvaz, Ahvaz, Iran, Faculty member of Payame Noor University, Tehran, Iran
2 Professor of Exercise Physiology, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3 Assistant Professor of Exercise Physiology, Shahid Chamran University of Ahvaz, Ahvaz, Iran
4 Associate Professor of Exercise Physiology, Shahid Chamran University of Ahvaz, Ahvaz, Iran
5 Professor of Exercise Physiology, University Of Guilan, Rasht, Iran
10.22089/spj.2018.5839.1768
Abstract
The purpose of the study was to investigate of effect of acute interval walking with blood flow restriction on 4EBP1, ERK and P38 proteins of skeletal muscle in inactive men. Five healthy sedentary were participated in 2 sessions including acute interval walking- BFR (5 intervals 3-min walking at 55% MHR and 1 min at rest with BFR) and acute interval walking (5 intervals 3-min walking at 55% MHR and 1 min at rest without BFR). Sampling of Vastus lateralis muscle before and 3 hours after the training from the right foot of the subjects at a distance of 5 to 10 cm. 4EBP1, ERK and P38 proteins concentration evaluated by Western blotting. Dependent t-test and and Independent t-test was used to analyze the data after subtracting the post-test score from the pre-test. However, there was a significant difference between the pre and post test for 4EBP1 (P = 0.001) and ERK (P = 0.049) in the blood flow restriction group. There was no significant difference between pre and post test of P38 (P = 0.452).significant difference was observed for ERK (P = 0.012) in acute interval walking.and (P = 0.049). There was no significant difference between pre and post test of 4EBP1(P = 0.064) and P38 (P = 0.122). No significant difference was found between two group for concentration of 4EBP1 (P=0.068), P38 (P-0.091) and ERK (P=0.827), (P ≥ 0.05). This study has shown that, Acute interval walking with blood flow restriction does not activate MAPK pathway signaling in inactive men.
Keywords
Walking
Blood Flow Restriction
Hypertrophy
Protein Synthesis

Main Subjects

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Sport Physiology
Volume 11, Issue 42 - Serial Number 42
Summer 2019
August 2019
Pages 47-60

  • Receive Date 18 May 2018
  • Revise Date 10 September 2018
  • Accept Date 11 September 2018