The Effects of Vitamin D and Continuous Training on Apoptosis Markers in Aorta Tissue of Rats Exposed to Hydrogen Peroxide (H2O2)

Document Type : Research Paper

Authors
Javid Mahtabi Alamdari 1
Hasan Matin Homaei 2
Parvin Farzanegi 3
1 Ph.D. Student of Sport Physiology, Islamic Azad University, Tehran Branch
2 Associate Professor of Sport Physiology, Islamic Azad University Tehran Branch
3 Associate Professor of Sport Physiology, Islamic Azad University, Sari Branch
10.22089/spj.2019.7061.1877
Abstract
The purpose of this study was to investigate the effect of vitamin D and continuous training on apoptotic markers of aorta tissue in poisoned water with oxygen. Thirty-six mature male Wistar rats (weighed on 220 ± 20gr, aged 8-10 weeks) were randomly assigned into 6 groups (n = 6) as follows; group 1; control group, group 2; received DMSO (Sham), group 3; Hydrogen peroxide, group 4; intraperitoneal injection of H2O2 + vitamin D, group 5; intraperitoneal injection of H2O2 + exercise, group 6; H2O2 + vitamin D + exercise. The animals were knocked out and the aortic tissue was isolated and the amounts of Caspase 3, Bax and Bcl2 proteins were measured by ELISA method. The data were analyzed by One-way ANOVA and P < 0.05 was considered as significant. Our results showed that the group which received only H2O2T significantly increased Bax, Caspase 3, and Bax/Bcl2 ratio compared to other groups (P < 0.05). also, we found that Vitamin D supplements, continuous training and combination of both reduced Bax, Caspase 3, and Bax/Bcl2 ratio, and increased Bcl2 levels compared to group which received only H2O2 (P < 0.05). According to the results, continue exercise with vitamin D supplementation decreased aorta by reducing Bax/Bcl2 ration and probably protects aorta tissue from damaging.
Keywords
Apoptosis
Aorta Tissue
Vitamin D
Caspase 3
Continuous Exercise
BCL2

Main Subjects

  1. Brüne B, Hartzell P, Nicotera P, Orrenius S. Spermine prevents endonuclease activation and apoptosis in thymocytes. Experimental cell research. 1991 Aug 1;195(2):323-9.
  2. Choy JC, Granville DJ, Hunt DW, McManus BM. Endothelial cell apoptosis: biochemical characteristics and potential implications for atherosclerosis. Journal of molecular and cellular cardiology. 2001 Sep 1;33(9):1673-90.
  3. Norouzi Kamareh MH, Zolfaghari MR, Ghaderi Pakdel F, Tolouei Azar J. Effect of 12 weeks moderate intensity resistance training and green tea extract on cardiac caspase-3 expression and telomerase enzyme content in aged male rats. SPJ. 2018;10(39): 107-26. (in Persian).
  4. Elmore S. Apoptosis: a review of programmed cell death. Toxicologic pathology. 2007 Jun;35(4):495-516.
  5. Alyami A, Soares MJ, Sherriff JL, Mamo JC. Vitamin D & endothelial function. The Indian journal of medical research. 2014 Oct;140(4):483.
  6. Halder SK, Sharan C, Al-Hendy A. 1, 25-dihydroxyvitamin D3 treatment shrinks uterine leiomyoma tumors in the Eker rat model. Biology of reproduction. 2012 Apr 1;86(4):116-.
  7. Lai YH, Fang TC. The pleiotropic effect of vitamin D. ISRN nephrology. 2013 Sep 4;2013.
  8. Koundourakis NE, Avgoustinaki PD, Malliaraki N, Margioris AN. Muscular effects of vitamin D in young athletes and non-athletes and in the elderly. Hormones. 2016 Oct 1;15(4):471-88.
  9. Yao T, Ying X, Zhao Y, Yuan A, He Q, Tong H, Ding S, Liu J, Peng X, Gao E, Pu J. Vitamin D receptor activation protects against myocardial reperfusion injury through inhibition of apoptosis and modulation of autophagy. Antioxidants & redox signaling. 2015 Mar 10;22(8):633-50..
  10. Michos ED, Blumenthal RS. Vitamin D supplementation and cardiovascular disease risk..
  11. Motiwala SR, Wang TJ. Vitamin D and cardiovascular risk. Curr Hypertens Rep. 2012 Jun; 14(3): 209-18. Doi:10.1007/s11906-012-0262-y
  12. Verdoia M, Schaffer A, Sartori C, Barbieri L, Cassetti E, Marino P, Galasso G, De Luca G, Novara Atherosclerosis Study Group (NAS). Vitamin D deficiency is independently associated with the extent of coronary artery disease. European journal of clinical investigation. 2014 Jul;44(7):634-42..
  13. Coyle CH, Martinez LJ, Coleman MC, Spitz DR, Weintraub NL, Kader KN. Mechanisms of H2O2-induced oxidative stress in endothelial cells. Free Radical Biology and Medicine. 2006 Jun 15;40(12):2206-13..
  14. Sayin O, Arslan N, Altun ZS, AKDOĞAN G. In vitro effect of resveratrol against oxidative injury of human coronary artery endothelial cells. Turkish Journal of Medical Sciences. 2011 Mar 16;41(2):211-8.
  15. Kwak HB, Song W, Lawler JM. Exercise training attenuates age-induced elevation in Bax/Bcl-2 ratio, apoptosis, and remodeling in the rat heart. The FASEB Journal. 2006 Apr;20(6):791-3
  16. Lee YI, Cho JY, Kim MH, Kim KB, Lee DJ, Lee KS. Effects of exercise training on pathological cardiac hypertrophy related gene expression and apoptosis. European journal of applied physiology. 2006 May 1;97(2):216-24.
  17. Lennon SV, Martin SJ, Cotter TG. Dose‐dependent induction of apoptosis in human tumour cell lines by widely diverging stimuli. Cell proliferation. 1991 Mar;24(2):203-14..
  18. Li SF, Liu HX, Zhang YB, Yan YC, Li YP. The protective effects of α-ketoacids against oxidative stress on rat spermatozoa in vitro. Asian journal of andrology. 2010 Mar;12(2):247.-56
  19. Liu H, Yang Z, Hu J, Luo Y, Zhu L, Yang H, Li G. Improvement of thoracic aortic vasoreactivity by continuous and intermittent exercise in high‑fat diet-induced obese rats. Biomedical reports. 2015 Jul 1;3(4):527-32
  20. Peterson JM, Bryner RW, Sindler A, Frisbee JC, Alway SE. Mitochondrial apoptotic signaling is elevated in cardiac but not skeletal muscle in the obese Zucker rat and is reduced with aerobic exercise. J appl physiol. 2008 Dec;105(6):1934-43.
  21. Quindry JC, Miller L, McGinnis G, Kliszczewicz B, Irwin JM, Landram M, Urbiztondo Z, Nanayakkara G, Amin R. Ischemia reperfusion injury, KATP channels, and exercise-induced cardioprotection against apoptosis. J of Appl Physiol. 2012 May 31;113(3):498-506.
  22. Radák Z, Sasvári M, Nyakas C, Pucsok J, Nakamoto H, Goto S. Exercise preconditioning against hydrogen peroxide-induced oxidative damage in proteins of rat myocardium. Archives of biochemistry and biophysics. 2000 Apr 15;376(2):248-51
  23. Akbari M, Shahidi F, Rajabi H, Kashef M, Mazaheri Z. The simultaneous effect of six weeks forced swimming and crocin supplementation on the expression of 3-cardiomyocyte genesase 3 in male rats infected with hydrogen peroxide. Razi JMS. 2018;25(9):26-37
  24. Marefeti H, Aminzadeh S, Najafipour H, Dabiri SH, Shahouzehi B. The effect of moderate-intensity interval training on the resistance to induced cardiac ischemiain adult male rate. Qom Univ Med Sci J. 2016;10(4):1-9.
  25. Martinez JE, Taipeiro ED, Chies AB. Effects of continuous and accumulated exercise on endothelial function in rat aorta. Arquivos brasileiros de cardiologia. 2017 Apr;108(4):315-22
  26. Amini A, Gaeini A, Chobineh S, Kordi MR, Alizadeh S. The effects of aerobic training on expression of BcL2 and miR-15 and BcL2 protein in tumor tissue in mice with breast cancer. Sport Physiol 2017; 8(32):85-100.
  27. Braga VA, Couto GK, Lazzarin MC, Rossoni LV, Medeiros A. Aerobic exercise training prevents the onset of endothelial dysfunction via increased nitric oxide bioavailability and reduced reactive oxygen species in an experimental model of menopause. PLoS One. 2015 Apr 29;10(4):e0125388
  28. Gu Q, Wang B, Zhang XF, Ma YP, Liu JD, Wang XZ. Chronic aerobic exercise training attenuates aortic stiffening and endothelial dysfunction through preserving aortic mitochondrial function in aged rats. Experimental gerontology. 2014 Aug 1;56:37-44.
  29. Guizoni DM, Dorighello GG, Oliveira HC, Delbin MA, Krieger MH, Davel AP. Aerobic exercise training protects against endothelial dysfunction by increasing nitric oxide and hydrogen peroxide production in LDL receptor-deficient mice. Journal of translational medicine. 2016 Dec;14(1):213.
  30. Kwak HB. Effects of aging and exercise training on apoptosis in the heart. Journal of exercise rehabilitation. 2013 Apr;9(2):212-19.
  31. Kwak HB, Song W, Lawler JM. Exercise training attenuates age-induced elevation in Bax/Bcl-2 ratio, apoptosis, and remodeling in the rat heart. The FASEB Journal. 2006 Apr;20(6):791-3
  32. Jafari A, Pourrazi H, Nikookheslat S, Baradaran B. Effect of exercise training on Bcl-2 and bax gene expression in the rat heart. Gene, Cell and Tissue. 2015;2(4) e60174.
  33. Liu WY, He W, Li H. Exhaustive training increases uncoupling protein 2 expression and decreases Bcl-2/Bax ratio in rat skeletal muscle. Oxidative medicine and cellular longevity. 2013 Jan 9;2013 DOI:10.1155/2013/780719.
  34. Ding Y, Liao W, Yi Z, Xiang W, He X. Cardioprotective role of vitamin D receptor in circulating endothelial cells of ApoE-deficient mice. International journal of clinical and experimental medicine. 2015;8(4):5065-74.
  35. Suzanne J, Vin T. Vitamin D deficiency and risk for cardiovascular disease. Am J Med Sci. 2009,338(1):40-4.
  36. Uberti F, Lattuada D, Morsanuto V, Nava U, Bolis G, Vacca G, et al. Vitamin D protects human endothelial cells from oxidative stress through the autophagic and survival pathways. J Clin Endocrinol Metab. 2014;99(4):1367-74.
  37. Marchesi LH, Costa IR, Ferreira MP, Nascimento JB, Ramos RD, Geraldo J, and et al Exercise training prior to myocardial infarction attenuates cardiac deterioration and cardiomyocyte dysfunction in rats. Clinics. 2013;68(4):549-56.
  38. Lu k, Wang L, Wang C, Yang Y, Hu D, Ding R. Effects of high-intensity interval versus continuous moderate-intensity aerobic exercise on apoptosis, oxidative stress and metabolism of the infarcted myocardium in a rat model. Mol Med Rep. 2015;12:2374-82.
  39. Ross MD, Malone E, Florida-James G. Vascular ageing and exercise: focus on cellular reparative processes. Oxidative Medicine and Cellular Longevity. 2016;2016.
  40. Marsh SA, Coombes JS. Exercise and the endothelial cell. International journal of cardiology. 2005 Mar 18;99(2):165-9.
  41. Vainshtein A, Kazak L, Hood DA. Effects of endurance training on apoptotic susceptibility in striated muscle. Journal of applied physiology. 2011 Apr 7;110(6):1638-45.
  42. Jin H, Yang R, Li W, Lu H, Ryan AM, Ogasawara AK, Van Peborgh J, Paoni NF. Effects of exercise training on cardiac function, gene expression, and apoptosis in rats. American Journal of Physiology-Heart and Circulatory Physiology. 2000 Dec 1;279(6):H2994-3002.
  43. Souza RR, de França E, Madureira D, Pontes CC, Santana JO, Caperuto EC. Resistance training improves aortic structure in Wistar rats. Brazilian journal of physical therapy. 2017 Jul 1;21(4):244-50.
  44. Marsh SA, Laursen PB, Pat BK, Gobe GC, Coombes JS. Bcl-2 in endothelial cells is increased by vitamin E and α-lipoic acid supplementation but not exercise training. Journal of molecular and cellular cardiology. 2005 Mar 1;38(3):445-51.
  45. Ribeiro F, Alves AJ, Duarte JA, Oliveira J. Is exercise training an effective therapy targeting endothelial dysfunction and vascular wall inflammation?. International journal of cardiology. 2010 Jun 11;141(3):214-21.
  46. Kim SY, Lee J. Exercise Training suppresses vascular fibrosis in aging obesity induced rats. Journal of exercise nutrition & biochemistry. 2014 Jun;18(2):175.
Sport Physiology
Volume 10, Issue 40
Winter 2019
January 2019
Pages 163-178

  • Receive Date 15 February 2019
  • Revise Date 26 May 2019
  • Accept Date 01 June 2019