Alterations in Enzymatic and Non‐Enzymatic Antioxidants Levels After One Session of Repeated-Sprint Activity and Cold Water Immersion

Document Type : Research Paper

Authors
Nima Gharahdaghi 1
Mohammad Reza Kordi 2
Fatemeh Shabkhiz 2
Sara Farajnia 3
Mojtaba Khaki 4
Saman Hajizadeh 3
1 Ph.D. of Exercise Physiology, Faculty of Physical Education and Sports Sciences, University of Tehran, Iran
2 Associate Professor of Exercise Physiology, Faculty of Physical Education and Sports Sciences, University of Tehran, Iran
3 M.Sc. of Exercise Physiology, Faculty of Physical Education and Sports Sciences, University of Tehran, Iran
4 Ph.D. Student of Exercise Physiology, Faculty of Physical Education and Sports Sciences, University of Tehran, Iran
10.22089/spj.2017.3320.1453
Abstract
The purpose of this study was to measure the changes in oxidized (GSSG) and reduced (GSH) glutathione, glutathione reductase (GR) and glutathione peroxidase (GPX) after repeated-sprint activity (RSA) and subsequent cold water immersion (CWI). Twenty trained athletes with maximal oxygen uptake (VO2max) 52.9±2.9 ml.kg-1.min-1, age 21.9±2.2 yrs, height 174.2±5.4 cm, and weight 68±4.4 kg was assigned to take part in this study. After performing repeated-sprint activity, 10 participants immersed in cold water (14°c) and 10 participants passively sat on a chair at room temperature. Blood sampling was performed before and after RSA, after CWI or passive rest and after 24 h. The blood variables assessed through ELISA method. The results showed that antioxidant levels did not return to baseline levels and CWI had no effect on these factors, compared to GPX and GR returned to baseline levels after 24 h, nevertheless; there was no significant difference between CWI and Control groups. In conclusion, although the levels of enzymatic and non‐enzymatic antioxidants were significantly higher after RSA, CWI did not have any effect on returning to baseline levels after 24h.
Keywords
Glutathione
Recovery
Antioxidant
Enzyme

Main Subjects

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Sport Physiology
Volume 12, Issue 47
Fall 2020
September 2020
Pages 133-146

  • Receive Date 16 November 2016
  • Revise Date 14 May 2016
  • Accept Date 26 December 2016