The Effect of Aerobic Exercise with Medium and High Intensity on the Gene Expression of Bax (BCL2 Associated X) and Bcl-2 (B-Cell Lymphoma 2) Markers in Rat Myocard After Ischemic-Reperfusion

Document Type : Research Paper

Authors

1 Assistant Professor of Exercise Physiology, Behbahan University of Medical Sciences

2 Professor of Exercise Physiology, Kharazmi University

3 Associate Professor of Exercise Physiology, Kharazmi University

4 Ph.D. Student in Animal Biology, Shahed University, Tehran

Abstract

The present research aims to examine the effect of aerobic exercise with medium and high intensity on the gene expression of Bax and Bcl-2 markers in rat myocard after ischemic-reperfusion by isoprenaline. 26 male Wistar rats were used weighing approximately 225.25±25.25 g with two to three months old; Then pilot study with stroke group (n = 7) and control Group (n = 7) was knock to examine of isopernalin induced-infarct and the infarct of left ventricle were confirmed in isopernalin into normal groups used Masson-trichrom teqnique. Then rats were divided into three groups to Measure gene expression: control (n=4), aerobic exercise with middle (n=4) and many intensity (n=4). The rats in research group were introduced to treadmill for a week, then the rats were exposed medium and high intensity aerobic training with frequency of three times weekly for one month. After two days of rest, rats were injected with isoprenaline within 24 hours; but control group were injected only with isopernaline in the end of one month. The formula 2-ΔΔct were used to measure the gene expression of BAX and BCL2, and independent t-test was used to compare the two groups of stroke and control in the pilot study and ANOVA were used to compare the groups.The results of study showed that aerobic exercise with medium and high intensity could reduce and increase the gene expression of BAX and BCL2, but these changes were not significant. Therefore, it seems that this intensity and period of training can not active anti-apoptotic routes of exercise to minimize the effects of stroke.

Keywords

Main Subjects

References

  1. Longo F, Kasper H, Loscalzo, J. Harrison's principles of internal medicine. 18th ed. New York, NY: McGraw-Hill Professional; 2012. p. 104-5.
  2. Sheryatzadeh M. Apoptosis programmed cell death. Arak: Arak University Press; 2009. p. 34-5.
  3. Santana ET, Serra AJ, Junior J, Bocalini D, Barauna V, Krieger J, et al. Aerobic exercise training induces an anti-apoptotic milieu in myocardial tissue. Motriz: Revista de Educação Física. 2014;20:233-8.
  4. Hyo-Bum kwak, Song W, Lawler JM. Exercise training attenuates age-induced elevation in Bax/Bcl-2 rati, apoptosis, and remodeling in the rat heart. The FASEB Journal. 2006;20(6):791-3.
  5. Delchev S, Georgieva K, Koeva Y, Atanassova P. Bcl-2/Bax ratio, mitochondrial membranes and aerobic enzyme activity in cardiomyocytes of rats after submaximal training. Folia Med (Plovdiv) 2006;48:50-6.
  6. Lajoie C, Calderone A, Béliveau L. Exercise training enhanced the expression of myocardial proteins related to cell protection in spontaneously hypertensive rats. Pflügers Archiv. 2004;449(1):26-32.
  7. Jafari A, Pourrazi H, Nikookheslat S, Baradaran B. Effect of exercise training on Bcl-2 and Bax gene expression in the rat heart. Gene Cell Tissue. 2015;2(4): 1-5.
  8. Marsh SA, Laursen PB, Pat BK, Gobe GC, Coombes JS. Bcl-2 in endothelial cells is increased by vitamin E and α-lipoic acid supplementation but not exercise training. Journal of Molecular and Cellular Cardiology. Journal of Molecular and Cellular Cardiology. 2005;38(3):445-51.
  9. Sagiv M. Exercise cardiopulmonary function in cardiac patients. London: Springer-Verlag; 2012. p. 205-55.
  10. Pescatello LS. ACSM’s guidelines for exercise testing and prescription. Baltimore: Wolters Kluwer/Lippincott Williams & Wilkins Health; 2014. p. 244-5.
  11. Zaheri S, Tadibi V, Monazami A., Naaleini F. Acute effects of an interval aerobic session on flow-mediated dilation and blood pressure in women with prehypertension. Sport Physiology. 2017;34(9):35-48.
  12. Azamianjazi A, Haffezi M, Cheraghi J, Abdi H. The Combined effect of endurance training and atorvastatin on the extent of necrosis damageand fibrosis tissue in male Wistar rats heart after experimental myocardial infarction. Medilam. 2016;23(7):28-38.
  13. Høydal MA, Wisløff U, Kemi OJ, Ellingsen O. Running speed and maximal oxygen uptake in rats and mice: practical implications for exercise training. European Journal of Cardiovascular Prevention & Rehabilitation. 2007;14(6):753-60.
  14. Vibha L, Asdaq S. Nagpal S, Rawri R. Protective effect of medicinal garlic against isoprenaline induced myocardial infarction in rats. International Journal of Pharmacology. 2011;7:510-5.
  15. Shahbaz AU, Zhao T, Zhao W, Johnson PL, Ahokas RA, et al. Calcium and zinc dyshomeostasis during isoproterenol-induced acute stressor state. Am J Physiol Heart Circ Physiol, 2011;300(2): 636-44.
  16. Jin Ha S, Weon Kim W. Mechanism of ischemia and reperfusion injury to the heart: From the viewpoint of nitric oxide and mitochondria. Chonnam Medical JournaL. 2010;46(3):129-39.
  17. Cooke MS, Evans MD, Dizdaroglu M, Lunec J. Oxidative DNA damage: Mechanisms, mutation, and disease. Faseb J. 2003;17(10):1195-214.
  18. Guizoni DM, Oliveira-Junior SA, Noor SL, Pagan LU, Martinez PF, et al. Effects of late exercise on cardiac remodeling and myocardial calcium handling proteins in rats with moderate and large size myocardial infarction. Int J Cardiol. 2016;221:406-12.
  19. de Sousa CV, Sales MM, Rosa TS, Lewis JE, de Andrade RV, et al. The antioxidant effect of exercise: A systematic review and meta-analysis. Sports Med. 2017;47(2):277-93.
  20. Kang PM, Yue P, Liu Z, Tarnavski O, Bodyaket N, ET al. Alterations in apoptosis regulatory factors during hypertrophy and heart failure. Am J Physiol Heart Circ Physiol, 2004;287(1): 72-80.
  21. Marfe G, Manzi V, Tafani M, Pucci B, Gambacurtaet A, et al. The modulation of sirtuins and apoptotic proteins in rats after exhaustive exercise. Open Journal of Molecular and Integrative Physiology. 2012;2:65-74.
  22. Chengji W, Shoujun H. Aerobic exercise can ameliorate heart function in patients with myocardial infarction through up-regulating M3 receptor. IJC Metabolic & Endocrine. 2016;13(Supplement C):1-5.
  23. Scott K Powers, Quindry JC, Kavazis AN. Exercise-induced cardioprotection against myocardial ischemia–reperfusion injury. Free Radical Biology and Medicine. 2008;44(2):193-201.
Sport Physiology
Volume 12, Issue 45
Spring 2020
April 2020
Pages 31-44

Files

History

  • Receive Date: 02 August 2017
  • Revise Date: 04 December 2017
  • Accept Date: 02 January 2018

Share

How to cite

Statistics