Muscle Murf1 and P70S6K Before and After 6 Weeks of Resistance Training and HMB Supplementation in Inactive Men

Document Type : Research Paper

Authors

1 Ph.D. Student of Exercise Physiology, Shahid Beheshti University, Tehran

2 Associate Professor of Exercise Physiology, Shahid Beheshti University, Tehran

3 Associate Professor of Exercise Physiology, University of Tehran

Abstract
Resistance training with HMB supplement helps preserving the body mass and, that is why, it is extensively practiced by athletes. However, there are contradictory results about the effect of the pathway of this supplement on increasing factors related to hypertrophy or reduction of atrophy. The present study investigates the effect of HMB and resistance training on response to Muscle MuRF1 and P70S6K in inactive men. To this end, 40 inactive men with the average age (25.5±5 years) and a BMI of (21.2±2 kg/m2) were divided into the following four training categories: training, training+HMB, HMB and control. The subjects did bodybuilding exercises (3set/10-12repitations/75%-80%1RM) for four days a week over a six-week time period. The control group did not do any kinds of exercise. The vastus lateralis muscle biopsy and 1RM amounts of each subject in leg press movement were conducted and measured before and after six weeks of training and consuming the supplement. The results of one-way ANOVA and repeated variance analysis test with inter-group factor showed that there is a difference (P= 0.001) between the increase of 1RM before and after six weeks of training and the amounts of P70S6K (P= 0.001) in all three groups in comparison with the control group. However, this difference was not significant (P= 0.22) in MuRF1 amounts. As a result, consuming HMB supplement in the course of training can lead to the increase of P70S6K response as a marker of hypertrophy and an increase of strength in the leg press after six weeks.

Keywords

Main Subjects

  1.  Spineti J, de Salles BF, Rhea MR, Lavigne D, Matta T, Miranda F, et al. Influence of exercise order on maximum strength and muscle volume in nonlinear periodized resistance training. Journal of strength and conditioning research/National Strength & Conditioning Association. 2010;24(11):2962-9.
  2. Paulo CA, Roschel H, Ugrinowitsch C, Kobal R, Tricoli V. Influence of different resistance exercise loading schemes on mechanical power output in work to rest ratio–equated and–nonequated conditions. The Journal of Strength & Conditioning Research. 2012;26(5):1308-12.
  3. Damas F, Phillips S, Vechin FC, Ugrinowitsch C. A review of resistance training-induced changes in skeletal muscle protein synthesis and their contribution to hypertrophy. Sports Medicine. 2015;45(6):701-8.
  4. Cheema BS, Chan D, Fahey P, Atlantis E. Effect of progressive resistance training on measures of skeletal muscle hypertrophy, muscular strength and health-related quality of life in patients with chronic kidney disease: A systematic review and meta-analysis. Sports Medicine. 2014;44(8):1125-38.
  5. Wu H, Xia Y, Jiang J, Du H, Guo X, Liu X, et al. Effect of beta-hydroxy-beta-methylbutyrate supplementation on muscle loss in older adults: A systematic review and meta-analysis. Archives of gerontology and geriatrics. 2015;61(2):168-75.
  6. Molfino A, Gioia G, Rossi Fanelli F, Muscaritoli M. Beta-hydroxy-beta-methylbutyrate supplementation in health and disease: A systematic review of randomized trials. Amino Acids. 2013;45(6):1273-92.
  7.  Fitschen PJ, Wilson GJ, Wilson JM, Wilund KR. Efficacy of β-hydroxy-β-methylbutyrate supplementation in elderly and clinical populations. Nutrition. 2013 Jan 1;29(1):29-36.
  8.  Wilson GJ, Wilson JM, Manninen AH. Effects of beta-hydroxy-beta-methylbutyrate (HMB) on exercise performance and body composition across varying levels of age, sex, and training experience: A review. Nutrition & metabolism. 2008 Dec;5(1):1-7.
  9. Portal S, Zadik Z, Rabinowitz J, Pilz-Burstein R, Adler-Portal D, Meckel Y, et al. The effect of HMB supplementation on body composition, fitness, hormonal and inflammatory mediators in elite adolescent volleyball players: a prospective randomized, double-blind, placebo-controlled study. European journal of applied physiology. 2011;111(9):2261-9.
  10. Pinheiro CH, Gerlinger-Romero F, Guimaraes-Ferreira L, de Souza-Jr AL, Vitzel KF, Nachbar RT, et al. Metabolic and functional effects of beta-hydroxy-beta-methylbutyrate (HMB) supplementation in skeletal muscle. European journal of applied physiology. 2012;112(7):2531-7.
  11. Zanchi NE, Gerlinger-Romero F, Guimaraes-Ferreira L, de Siqueira Filho MA, Felitti V, Lira FS, et al. HMB supplementation: clinical and athletic performance-related effects and mechanisms of action. Amino Acids. 2011;40(4):1015-25.
  12. Sunami T, Byrne N, Diehl RE, Funabashi K, Hall DL, Ikuta M, et al. Structural basis of human p70 ribosomal S6 kinase-1 regulation by activation loop phosphorylation. The Journal of Biological Chemistry. 2010;285(7):4587-94.
  13. Basualto-Alarcon C, Jorquera G, Altamirano F, Jaimovich E, Estrada M. Testosterone signals through mTOR and androgen receptor to induce muscle hypertrophy. Medicine and Science in Sports and Exercise. 2013;45(9):1712-20.
  14. Durkalec-Michalski K, Jeszka J. The efficacy of a β-hydroxy-β-methylbutyrate supplementation on physical capacity, body composition and biochemical markers in elite rowers: a randomised, double-blind, placebo-controlled crossover study. Journal of the International Society of Sports Nutrition. 2015 Dec 1;12(1):31.
  15. Centner T, Yano J, Kimura E, McElhinny AS, Pelin K, Witt CC, et al. Identification of muscle specific ring finger proteins as potential regulators of the titin kinase domain. Journal of Molecular Biology. 2001;306(4):717-26.
  16. Bodine SC, Baehr LM. Skeletal muscle atrophy and the E3 ubiquitin ligases MuRF1 and MAFbx/atrogin-1. American Journal of Physiology Endocrinology and Metabolism. 2014;307(6):469-84.
  17. Noh KK, Chung KW, Choi YJ, Park MH, Jang EJ, Park CH, et al. Beta-hydroxy beta-methylbutyrate improves dexamethasone-induced muscle atrophy by modulating the muscle degradation pathway in SD rat. PloS one. 2014;9(7):102947.
  18. Kao M, Columbus DA, Suryawan A, Steinhoff-Wagner J, Hernandez-Garcia A, Nguyen HV, et al. Enteral beta-hydroxy-beta-methylbutyrate supplementation increases protein synthesis in skeletal muscle of neonatal pigs. American Journal of Physiology Endocrinology and Metabolism. 2016;310(11):1072-84.
  19. Riebe D, Ehrman JK, Liguori G, Magal M, American College of Sports Medicine, editors. ACSM's guidelines for exercise testing and prescription. Wolters Kluwer; 2018.
  20. Asadi A, Arazi H, Suzuki K. Effects of β-hydroxy-β-methylbutyrate-free acid supplementation on strength, power and hormonal adaptations following aresistance training. Nutrients. 2017 Dec;9(12):1316.
  21. Kraemer WJ, Hatfield DL, Comstock BA, Fragala MS, Davitt PM, Cortis C, et al. Influence of HMB supplementation and resistance training on cytokine responses to resistance exercise. Journal of the American College of Nutrition. 2014;33(4):        247-55.
  22. Kraemer WJ, Ratamess NA. Fundamentals of resistance training: Progression and exercise prescription. Medicine and Science in Sports and Exercise. 2004;36(4):    674-88.
  23. Kraemer WJ, Adams K, Cafarelli E, Dudley GA, Dooly C, Feigenbaum MS, et al. American College of Sports Medicine position stand. Progression models in resistance training for healthy adults. Medicine and Science in Sports and Exercise. 2002;34(2):364-80.
  24. Kraemer WJ, Hatfield DL, Volek JS, Fragala MS, Vingren JL, Anderson JM, et al. Effects of amino acids supplement on physiological adaptations to resistance training. Medicine & Science in Sports & Exercise. 2009;41(5):1111-21.
  25. Allison AG. The effects of a 12-week resistance training program combined with casein or whey protein supplementation on body composition, muscle strength, and markers of satellite cell activation in older males (Doctoral dissertation)
  26. Kraemer WJ, Ratamess NA. Hormonal responses and adaptations to resistance exercise and training. Sports Medicine. 2005;35(4):339-61.
  27. Routhier DD, Stacy JJ. HMB use and its relationship to exercise-induced muscle damage and performance during exercise. International SportMed Journal. 2007;8(2):68-77.
  28. Szcześniak K, Ostaszewski P, Fuller Jr J, Ciecierska A, Sadkowski T. Dietary supplementation of β‐hydroxy‐β‐methylbutyrate in animals: A review. Journal of Animal Physiology and Animal Nutrition. 2015;99(3):405-17.
  29. Krug AL, Macedo AG, Zago AS, Rush JW, Santos CF, Amaral SL. High-intensity resistance training attenuates dexamethasone-induced muscle atrophy. Muscle & Nerve. 2016;53(5):779-83.
  30. Munroe M, Pincu Y, Merritt J, Cobert A, Brander R, Jensen T, et al. Impact of beta-hydroxy beta-methylbutyrate (HMB) on age-related functional deficits in mice. Experimental Gerontology. 2017;87(Pt A):57-66.
  31. Su YH, Su Z, Zhang K, Yuan QK, Liu Q, Lv S, et al. [The changes of p-Akt/MuRF1/FoxO1 proteins expressions in the conditions of training and immobilization in rats' gastrocnemius muscle]. Sheng li xue bao :[Acta physiologica Sinica]. 2014;66(5):589-96.
  32. Gerlinger-Romero F, Guimaraes-Ferreira L, Yonamine CY, Salgueiro RB, Nunes MT. Effects of beta-hydroxy-beta-methylbutyrate (HMB) on the expression of ubiquitin ligases, protein synthesis pathways and contractile function in extensor digitorum longus (EDL) of fed and fasting rats. The Journal of Physiological Sciences. 2018 Mar 1;68(2):165-74.
  33. Kornasio R, Riederer I, Butler-Browne G, Mouly V, Uni Z, Halevy O. Beta-hydroxy-beta-methylbutyrate (HMB) stimulates myogenic cell proliferation, differentiation and survival via the MAPK/ERK and PI3K/Akt pathways. Biochimica et Biophysica Acta. 2009;1793(5):755-63.
  34. Hawley JA, Hargreaves M, Joyner MJ, Zierath JR. Integrative biology of exercise. Cell. 2014;159(4):738-49.
  35. Albert FJ, Morente-Sánchez J, Ortega FB, Castillo MJ, Gutiérrez Á. Usefulness of β-hydroxy-β-methylbutyrate (HMB) supplementation in different sports: An update and practical implications. Nutricion Hospitalaria. 2015;32(1):20-33.
  36. Eley HL, Russell ST, Baxter JH, Mukerji P, Tisdale MJ. Signaling pathways initiated by beta-hydroxy-beta-methylbutyrate to attenuate the depression of protein synthesis in skeletal muscle in response to cachectic stimuli. American Journal of Physiology Endocrinology and Metabolism. 2007;293(4):923-31.
  37. Nakada S, Ogasawara R, Kawada S, Maekawa T, Ishii N. Correlation between ribosome biogenesis and the magnitude of hypertrophy in overloaded skeletal muscle. PloS one. 2016;11(1):0147284.
  38. Arazi H, Asadi A, Suzuki K. The effects of beta-hydroxy-beta-methylbutyrate-free acid supplementation and resistance training on oxidative stress markers: A randomized, double-blind, placebo-controlled study. Antioxidants. 2018 Jun;7(6):76.
  39. Wilson JM, Lowery RP, Joy JM, Andersen JC, Wilson SM, Stout JR, et al. The effects of 12 weeks of beta-hydroxy-beta-methylbutyrate free acid supplementation on muscle mass, strength, and power in resistance-trained individuals: A randomized, double-blind, placebo-controlled study. European Journal of Applied Physiology. 2014;114(6):1217-27.
  40. Wilson JM, Lowery RP, Joy JM, Walters JA, Baier SM, Fuller JC Jr., et al. beta-Hydroxy-beta-methylbutyrate free acid reduces markers of exercise-induced muscle damage and improves recovery in resistance-trained men. The British Journal of Nutrition. 2013;110(3):538-44.
  41. Sanchez-Martinez J, Santos-Lozano A, Garcia-Hermoso A, Sadarangani KP, Cristi-Montero C. Effects of beta-hydroxy-beta-methylbutyrate supplementation on strength and body composition in trained and competitive athletes: A meta-analysis of randomized controlled trials. Journal of Science and Medicine in Sport. 2018 Jul 1;21(7):727-35.

 

Sport Physiology
Volume 12, Issue 45
Spring 2020
April 2020
Pages 79-94

  • Receive Date 02 July 2018
  • Revise Date 22 December 2018
  • Accept Date 28 December 2018