Sport Physiology

Current Issue

By Issue

By Author

By Subject

Author Index

Keyword Index

About Journal

Aims and Scope

Editorial Board

Article Processing Charges (APC)

Indexing and Abstracting

Related Links

FAQ

Reviewers

Terms and conditions for manuscript submission

Submission Instruction

Forms

Peer Review Process

The Effect of Eight Weeks of Moderate and High Intensity Resistance Training on Muscular miR-1, miR-206 Expression and Serum IGF- I in Wistar Older Rats

Document Type : Research Paper

Authors

1 Ph.D. Student in Exercise Physiology, Shahrekord University

2 Professor of Exercise Physiology, Shahrekord University

3 Assistant Professor of Exercise Physiology, Shahrekord University

4 Assistant Professor of Biology, Shahrekord University

Abstract

One of the most important and prevalent consequences in elderly people is age-related muscular atrophy or Sarcopenia. Sarcopenia is associated with a significant reduction in muscle strength and mass. The purpose of this study was to investigate the effect of moderate and high intensity resistance training on miR-1, miR-206 expression and serum IGF-1 in elderly rats. 30 male Wistar rats (23 months old) were randomly divided into two experiment and one control group including moderate intensity resistance training (n = 9), high intensity resistance training (n = 8) and the control group (n =8. Resistance training included 8 weeks of climbing a ladder with high intensity (80% MVCC) and moderate intensity (60% of MVCC) and 5 days a week. After completing training, miR-1, miR-206 expression was measured using the by RT-PCR method in Soleus and FHL muscle and IGF-I in serum. The statistical analysis was performed using One Way ANOVA test with significance level of  (P <0.05). The results showed that in both resistance training group, miR-1 and miR-206 expression were significantly lower than those in control group and IGF-1 concentrations was significantly higher in both high and moderate resistance groups than control group        (P <0.05). High intensity resistance training was more effective for both miR-1 and miR-206 in FHL muscle and serumIGF-1. It seems that resistance training with moderate and high intensity can change the resting levels microRNAs related to muscular atrophy (miR-1, miR-206) and its target gene (IGF-1) in older rats and lead to the prevention of sarcopenia in older peoples. 

Keywords

Main Subjects

References
  1. Lang T, Streeper T, Cawthon P, Baldwin K, Taaffe DR, Harris T. Sarcopenia: Etiology, clinical consequences, intervention, and assessment. Osteoporosis international. 2010;21(4):543-59.
  2. Walrand S, Guillet C, Salles J, Cano N, Boirie Y. Physiopathological mechanism of sarcopenia. Clinics in geriatric medicine. 2011;27(3):365-85.
  3. Boirie Y. Physiopathological mechanism of sarcopenia. The Journal of Nutrition, Health and Aging. 2009;13(8):717-23.
  4. Miljkovic N, Lim JY, Miljkovic I, Frontera WR. Aging of skeletal muscle fibers. Annals of rehabilitation medicine. 2015;39(2):155-62.
  5. Philippou A, Maridaki M, Halapas A, Koutsilieris M. The role of the insulin-like growth factor 1 (IGF-1) in skeletal muscle physiology. In Vivo. 2007;21(1):45-54.
  6. Hitachi K, Tsuchida K. Role of microRNAs in skeletal muscle hypertrophy. Frontiers in physiology. 2014; 16;4:408:1-7.
  7. Machida S, Booth FW. Insulin-like growth factor 1 and muscle growth: implication for satellite cell proliferation. Proceedings of the Nutrition Society. 2004;63(2):      337-40.
  8. Huygens W, Thomis MA, Peeters MW, Aerssens J, Janssen R, Vlietinck RF, et al. Linkage of myostatin pathway genes with knee strength in humans. Physiological genomics. 2004;17(3):264-70.
  9. Goldspink G. Mechanical signals, IGF-I gene splicing, and muscle adaptation. Physiology. 2005;20(4):232-8.
  10. Cappon J, Brasel J, Mohan S, Cooper D. Effect of brief exercise on circulating insulin-like growth factor I. Journal of applied physiology. 1994;76(6):2490-6.
  11. Walker KS, Kambadur R, Sharma M, Smith HK. Resistance training alters plasma myostatin but not IGF-1 in healthy men. Medicine and science in sports and exercise. 2004;36(5):787-93.
  12. Seo D-I, Jun T-W, Park K-S, Chang H, So W-Y, Song W. 12 weeks of combined exercise is better than aerobic exercise for increasing growth hormone in middle-aged women. International journal of sport nutrition and exercise metabolism. 2010;20(1):21-6.
  13. Wang XH. MicroRNA in myogenesis and muscle atrophy. Current opinion in clinical nutrition and metabolic care. 2013; 16(3), p.258-66.
  14. Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 2004;116(2):281-97.
  15. Aoi W, Sakuma K. Does regulation of skeletal muscle function involve circulating microRNAs? Frontiers in physiology. 2014;5: p.39.
  16. De Lencastre A, Pincus Z, Zhou K, Kato M, Lee SS, Slack FJ. MicroRNAs both promote and antagonize longevity in C. elegans. Current Biology. 2010; 20(24):   2159-68.
  17. Chen J-F, Mandel EM, Thomson JM, Wu Q, Callis TE, Hammond SM, et al. The role of microRNA-1 and microRNA-133 in skeletal muscle proliferation and differentiation. Nature genetics. 2006; 38(2), 228-33.
  18. McCarthy JJ. The MyomiR network in skeletal muscle plasticity. Exercise and sport sciences reviews. 2011;39(3): 150-4.
  19. O'Neill C, Kiely AP, Coakley MF, Manning S, Long-Smith CM. Insulin and IGF-1 signalling: longevity, protein homoeostasis and Alzheimer's disease. : Portland Press Limited; 2012. p. 721-7.
  20. Rommel C, Bodine SC, Clarke BA, Rossman R, Nunez L, Stitt TN, et al. Mediation of IGF-1-induced skeletal myotube hypertrophy by PI (3) K/Akt/mTOR and PI (3) K/Akt/GSK3 pathways. Nature cell biology. 2001; 3(11), 1009-13.
  21. Spangenburg E, Booth F. Molecular regulation of individual skeletal muscle fibre types. Acta physiologica Scandinavica. 2003;178(4):413-24.
  22. Narici MV, Maffulli N. Sarcopenia: characteristics, mechanisms and functional significance. British medical bulletin. 2010;95(1):139-59.
  23. Lexell J, Taylor CC, Sjöström M. What is the cause of the ageing atrophy?: Total number, size and proportion of different fiber types studied in whole vastus lateralis muscle from 15-to 83-year-old men. Journal of the neurological sciences. 1988;    84(2-3): 275-94.
  24. Klitgaard H, Bergman O, Betto R, Salviati G, Schiaffino S, Clausen T, et al. Co-existence of myosin heavy chain I and IIa isoforms in human skeletal muscle fibres with endurance training. Pflügers Archiv. 1990; 416(4): 470-2.
  25. McCarthy JJ, Esser KA. MicroRNA-1 and microRNA-133a expression are decreased during skeletal muscle hypertrophy. Journal of applied physiology. 2007; 102(1): 306-13.
  26. Nielsen S, Scheele C, Yfanti C, Åkerström T, Nielsen AR, Pedersen BK, et al. Muscle specific microRNAs are regulated by endurance exercise in human skeletal muscle. The Journal of physiology. 2010; 588(20): 4029-37.
  27. Fallah A, Gharekhanlooi R, Soleimani M, Mojtahed S. The expression of miR-206 in response to one session resistance exercise in fast and slow twitch skeletal muscles of Wistar male rats. 2016:56-63.
  28. Allen DL, Bandstra ER, Harrison BC, Thorng S, Stodieck LS, Kostenuik PJ, et al. Effects of spaceflight on murine skeletal muscle gene expression. Journal of Applied Physiology. 2009; 106(2): 582-95.
  29. Drummond MJ, McCarthy JJ, Fry CS, Esser KA, Rasmussen BB. Aging differentially affects human skeletal muscle microRNA expression at rest and after an anabolic stimulus of resistance exercise and essential amino acids. American Journal of Physiology-Endocrinology and Metabolism. 2008; 295(6): 1333.
  30. Soci UPR, Fernandes T, Hashimoto NY, Mota GF, Amadeu MA, Rosa KT, et al. MicroRNAs 29 are involved in the improvement of ventricular compliance promoted by aerobic exercise training in rats. Physiological genomics. 2011; 43(11): 665-73.
  31. Huang Z, Chen X, Chen D. Myostatin: a novel insight into its role in metabolism, signal pathways, and expression regulation. Cellular signalling. 2011; 23(9): 1441-6.
  32. de Cássia Marqueti R, Almeida JA, Nakagaki WR, Guzzoni V, Boghi F, Renner A, et al. Resistance training minimizes the biomechanical effects of aging in three different rat tendons. Journal of biomechanics. 2017; 53, 29-35.
  33. Krug AL, Macedo AG, Zago AS, Rush JW, Santos CF, Amaral SL. High‐intensity resistance training attenuates dexamethasone‐induced muscle atrophy. Muscle & nerve. 2016; 53(5): 779-88.
  34. Macedo AG, Krug AL, Herrera NA, Zago AS, Rush JW, Amaral SL. Low-intensity resistance training attenuates dexamethasone-induced atrophy in the flexor hallucis longus muscle. The Journal of steroid biochemistry and molecular biology. 2014; 143: 357-64.
  35. Fathi M. The study of timing series response of microRNA-1 expression to resistance exercise in slow and fast muscles of Wistar male rats. (2013): 5-15.
  36. McCarthy JJ, Esser KA, Peterson CA, Dupont-Versteegden EE. Evidence of MyomiR network regulation of β-myosin heavy chain gene expression during skeletal muscle atrophy. Physiological genomics. 2009; 39(3): 219-26.
  37. Yan B, Zhu C-D, Guo J-T, Zhao L-H, Zhao J-L. miR-206 regulates the growth of the teleost tilapia (Oreochromis niloticus) through the modulation of IGF-1 gene expression. Journal of Experimental Biology. 2013; 216(7): 1265-9.
  38. Tofighi A, Dehkordi AJ, Tartibian B, Shourabeh FF, Sinaei M. Effects of aerobic, resistance, and concurrent training on secretion of growth hormone and insulin-like growth dactor-1 in elderly women. Journal of Isfahan Medical School. 2012; 30(184).
  39. Jensen GL. Inflammation: Roles in aging and sarcopenia. Journal of parenteral and enteral nutrition. 2008; 32(6): 656-9.
  40. Glass DJ. Skeletal muscle hypertrophy and atrophy signaling pathways. The international journal of biochemistry & cell biology. 2005; 37(10): 1974-84.
  41. Scheett TP, Nemet D, Stoppani J, Maresh CM, Newcomb R, Cooper DM. The effect of endurance-type exercise training on growth mediators and inflammatory cytokines in pre-pubertal and early pubertal males. Pediatric research. 2002; 52(4): 491.
  42. Pyka G, Lindenberger E, Charette S, Marcus R. Muscle strength and fiber adaptations to a year-long resistance training program in elderly men and women. Journal of Gerontology. 1994; 49(1), 22-7.
  43. McDonagh MJ, Davies C. Adaptive response of mammalian skeletal muscle to exercise with high loads. European journal of applied physiology and occupational physiology. 1984; 52(2): 139-55.
  44. Hickson R, Rosenkoetter M, Brown M. Strength training effects on aerobic power and short-term endurance. Medicine and Science in Sports and Exercise. 1980; 12(5): 336-9.
  45. Gettman LR, Pollock ML. Circuit weight training: A critical review of its physiological benefits. The Physician and Sportsmedicine. 1981; 9(1): 44-60.
Sport Physiology
Volume 12, Issue 46
July 2020
Pages 57-76
Files
History
  • Receive Date: 04 November 2018
  • Revise Date: 07 January 2019
  • Accept Date: 19 January 2019
Share
How to cite
Statistics