The Effect of Aerobic Exercise and Ethanol Consumption on Nrf2 Gene Expression in Heart Tissue and Some Antioxidant Indices in Male Rat

Document Type : Research Paper

Authors

1 M.Sc. Graduate in Exercise Physiology, Department of Exercise Physiology, Faculty of Sport Sciences, University of Mazandaran, Babolsar, Iran

2 Professor, Department of Exercise Physiology, Faculty of Sport Science, University of Mazandaran, Babolsar, Iran

3 Assistance Professor, Department of Exercise Physiology, Faculty of Sport Science, University of Mazandaran, Babolsar, Iran

4 Ph.D. Graduate in Exercise Physiology, Faculty of Sport Sciences, University of Mazandaran, Babolsar, Iran

Abstract

There have been limited studies on the effect of the combination of aerobic exercise and alcohol consumption on regulators of antioxidant defense systems, especially erythroid nuclear factor 2 associated with factor 2 (Nrf2) in heart muscle.The aim of this study was to investigate the effect of eight weeks of aerobic exercise with ethanol consumption on Nrf2 gene expression in heart tissue and antioxidant parameters of plasma in male rats. A number of 32 rats with a weighing average of 230 ± 6 g were divided into four groups control, aerobic exercise, ethanol 20% at a dose of 4 g/kgbw, and ethanol+ aerobic exercise. At the end of the period, levels of Nrf2 gene expression and total capacity antioxidant and malondialdehyde were evaluated. Data were analyzed by two-way ANOVA at the significant level of p≤0.05.The results showed that aerobic exercise had a significant effect on Nrf2 gene expression (P=0.0068).The results showed no significant effect of ethanol consumption (P=0.312) and interaction effect between aerobic exercise and ethanol consumption (P=0.237) on Nrf2 gene expression. Aerobic exercise significantly increased the expression of Nrf2 gene in the aerobic exercise and ethanol+ aerobic exercise groups compared to the control. Ethanol consumption significantly reduced TCA and increased MDA levels compared to other groups.The findings showed that ethanol consumption decreased and increased plasma levels of TAC and MDA, respectively. In contrast, aerobic exercise through increasing TAC levels and increase Nrf2 gene expression leads to a decrease in the oxidative damage caused by ethanol consumption.

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Main Subjects

References

  1. Organization WH. Global status report on alcohol and health 2018. World Health Organization; 2018. Available at: https://www.who.int/publications/i/item/9789241565639 [cited 2018 Sep 27]
  2. Manthou E, Georgakouli K, Fatouros IG, Gianoulakis C, Theodorakis Y, Jamurtas AZ. Role of exercise in the treatment of alcohol use disorders.  Biomed Rep.2016;4(5):535-45.
  3. World Health Organization. Management of Substance Abuse. Global status report on alcohol and health 2014. World Health Organization, 2014
  4. Fernandez-Sola J. Cardiovascular risks and benefits of moderate and heavy alcohol consumption. Nat Rev Cardiol 2015;12(10):576-87.
  5. Saravanan R, Pugalendi V. Impact of ursolic acid on chronic ethanol-induced oxidative stress in the rat heart. Pharmacol Rep. 2006;58(1):41-7.
  6. Nikaj A, Cakani B, Shkoza A, Ranxha E, Vyshka G. Effects of ethanol on the heart and blood vessels. OA Alcohol. 2014;2(1):7.
  7. Pollack M, Phaneuf S, Dirks A, Leeuwenburgh C. The role of apoptosis in the normal aging brain, skeletal muscle, and heart. Ann NY Acad Sci. 2002;959(1):93-107.
  8. Gardner JD, Mouton AJ. Alcohol effects on cardiac function. Compr Physiol. 2011;5(2):791-802.
  9. Ascensão A, Ferreira R, Magalhães J. Exercise-induced cardioprotection—biochemical, morphological and functional evidence in whole tissue and isolated mitochondria. Int J Cardiol. 2007;117(1):16-30.
  10. Sun J, Fu J, Li L, Chen C, Wang H, Hou Y, et al. Nrf2 in alcoholic liver disease. Toxicol Appl Pharmacol. 2018;357:62-9.
  11. Kandola K, Bowman A, Birch‐Machin M. Oxidative stress–a key emerging impact factor in health, ageing, lifestyle and aesthetics. IntJ Cosmet Sci. 2015;37:1-8.
  12. Nguyen T, Sherratt PJ, Pickett CB. Regulatory mechanisms controlling gene expression mediated by the antioxidant response element. Annu Rev Pharmacol Toxicol. 2003;43(1):233-60.
  13. Gounder SS, Kannan S, Devadoss D, Miller CJ, Whitehead KS, Odelberg SJ, et al. Impaired transcriptional activity of Nrf2 in age-related myocardial oxidative stress is reversible by moderate exercise training. PloS One. 2012;7(9):e45697.
  14. Adams AK, Best TM. The role of antioxidants in exercise and disease prevention. Phys Sportsmed. 2002;30(5):37-44.
  15. Bloomer RJ, Goldfarb AH, Wideman L, McKenzie MJ, Consitt LA. Effects of acute aerobic and anaerobic exercise on blood markers of oxidative stress. J Strength Cond Res. 2005;19(2):276-85.
  16. Davies KJ. Cardiovascular adaptive homeostasis in exercise. Front Physiol. 2018;9:369.
  17. Farney TM, Mccarthy CG, Canale RE, Schilling BK, Whitehead PN, Bloomer RJ. Absence of blood oxidative stress in trained men after strenuous exercise. Med Sci Sports Exerc. 2012;44(10):1855-63.
  18. de Lucca MS, Pereira ET, Righi T, de Carvalho CA, Israel C, Nogueira DNQdC, et al. Liver histology after chronic use of alcohol and exercise training in rats. J Pharm Pharmacol. 2018;6:52-60.
  19. Akbari A, Nasiri K, Heydari M, Mosavat SH, Iraji A. The protective effect of hydroalcoholic extract of Zingiber officinale Roscoe (Ginger) on ethanol-induced reproductive toxicity in male rats.  Evid Based Complement Alternat Med. 2017;22(4):609-17.
  20. Liang J, Li L, Sun Y, He W, Wang X, Su Q. The protective effect of activating Nrf2/HO-1 signaling pathway on cardiomyocyte apoptosis after coronary microembolization in rats. BMC Cardiovasc Disord. 2017;17(1):272.
  21. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem. 1996;239(1):70-6.
  22. Esterbauer H, Cheeseman KH. Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal.  Methods Enzymol. 1990;186: 407-21.
  23. Chicco AJ, McCarty H, Reed AH, Story RR, Westerlind KC, Turner RT, et al. Resistance exercise training attenuates alcohol-induced cardiac oxidative stress. Eur J Cardiovasc Prev Rehabil. 2006;13(1):74-9.
  24. Akbari A, Nasiri K, Heydari M, Nimrouzi M, Afsar T. Ameliorating potential of ginger (zingiber officinale roscoe) extract on liver function and oxidative stress induced by ethanol in male rats. Zahedan J Res Med Sci. 2019;21(2): e86464.
  25. Georgakouli K, Manthou E, Fatouros IG, Georgoulias P, Deli CK, Koutedakis Y, et al. Enhanced erythrocyte antioxidant status following an 8-week aerobic exercise training program in heavy drinkers. Alcohol. 2018;69:57-62.
  26. Wu D, Cederbaum AI. Alcohol, oxidative stress, and free radical damage. Alcohol Res Health.  2003;27:277-84.
  27. Shin Y-A, Lee J-H, Song W, Jun T-W. Exercise training improves the antioxidant enzyme activity with no changes of telomere length. Mech Ageing Dev 2008;129(5):254-60.
  28. Roh H-T, Ha HZ, Woo J-H, Lee Y-H, Ko K, Bae J-Y. Effect of different exercise intensities on biomarkers of oxidant-antioxidant balance, inflammation, and muscle damage. Journal of the Korean Applied Science and Technology. 2018;35(3):778-86.
  29. Alikhani S, Sheikholeslami‐Vatani D. Oxidative stress and anti‐oxidant responses to regular resistance training in young and older adult women. Geriatr Gerontol Int. 2019;19(5):419-22.
  30. Ji LL. Antioxidants and oxidative stress in exercise. Proc Soc Exp Biol Med. 1999;222(3):283-92.
  31. Brites FD, Evelson PA, Christiansen MG, Nicol MF, Basílico MJ, Wikinski RW, et al. Soccer players under regular training show oxidative stress but an improved plasma antioxidant status. Clin Sci. 1999;96(4):381-5.
  32. Balakrishnan S, Anuradha C. Exercise, depletion of antioxidants and antioxidant manipulation. Cell Biochem Funct. 1998;16(4):269-75.
  33. Narasimhan M, Rajasekaran NS. Exercise, Nrf2 and antioxidant signaling in cardiac aging. Front Physiol. 2016;7:241.
  34. Done AJ, Newell MJ, Traustadóttir T. Effect of exercise intensity on Nrf2 signalling in young men. Free Radic Res 2017;51(6):646-55.
  35. Majerczak J, Rychlik B, Grzelak A, Grzmil P, Karasinski J, Pierzchalski P, et al. Effect of 5-week moderate intensity endurance training on the oxidative stress, muscle specific uncoupling protein (UPC3) and superoxide dismutase (SOD2) contents in vastus lateralis of young healthy men. J Physiol Pharmacol. 2010;61(6):743-51.
  36. RAdak Z, Kaneko T, Tahara S, Nakamoto H, Ohno H, SASVari M, et al. The effect of exercise training on oxidative damage of lipids, proteins, and DNA in rat skeletal muscle: evidence for beneficial outcomes. Free Radic Biol Med. 1999;27(1-2):69-74.
  37. Ames BN, Shigenaga MK. Oxidants Are a Major Contributor to Aging a. Ann N Y Acad Sci. 1992;663(1):85-96.
  38. Powers SK, Quindry JC, Kavazis AN. Exercise-induced cardioprotection against myocardial ischemia–reperfusion injury. Free Radic Biol Med. 2008;44(2):193-201.

 

Sport Physiology
Volume 13, Issue 49
March 2021
Pages 65-88

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History

  • Receive Date: 11 July 2019
  • Revise Date: 12 August 2019
  • Accept Date: 16 September 2019

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