The Effect of Eight Weeks of High Intensity Interval Training on Ubiquitin-Proteasome System in Fast Twitch Muscle of Post Myocardial Infarction Rats

Document Type : Research Paper

Authors
Mohmad Reza Zolfeghari 1
Gholam Reza Nemati 2
Amir Fattahi 3
1 Assistant Professor of Exercise Physiology, Urmia University
2 Ph.D. Student of Exercise Physiology, Urmia University
3 Assistant Professor of Clinical Biochemistry, Tabriz University
10.22089/spj.2019.7825.1955
Abstract
Weakness, intolerance of exercise and eventually muscle atrophy are important features of patients with heart failure. Therefore, the purpose of this study was to investigate the effect of 8 weeks of high intensity interval training on ubiquitin-proteasome system in fast twitch muscle of post myocardial infarction (MI) rats. Methods: Myocardial infarction was induced by left artery descending ligation for 30 min. 32 male Wistar rats were randomly divided into 4 groups (n = 8): MI control (MI-CTL), high-intensity interval MI (MI-HIIT), high-intensity interval (HIIT) and Sham- operated. Exercise training was undergoing 40 min, 3 sessions per week for 8-weeks (85-90% VO2max). 24 hours after the last training session, the rats were anesthetized and their extensor digitorum longus (EDL) tissue removed. RT-PCR method was used to measuregene expression (NOX2, TNF-a, NF-KB, P38 MAPK, Murf-1) was used. Data analysis were performed using a one-way ANOVA and Tukey follow up tests, considering as significance level (P < 0.05). Results: 8-week HIIT significantly decreased NOX2, TNF-a in MI-HIIT compared with MI-CTL and Sham (P=0.03). These reductions wereparalleled a significant decrease in activity NF-KB, P38, Murf-1 in MI-HIIT compared with MI-CTL and Sham (P<0.05). Also, NOX2 change in HIIT group was significantly higher than MI-CTL and Sham (P<0.05). According to the results of HIIT probably as an effective treatment, it reduces muscle atrophy in fast muscle contraction of heart patients.
Keywords
High- Intensity Interval Training
Ubiquitin-Proteasome System
Myocardial Infarction
Fast Twitch Muscle

Main Subjects

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Sport Physiology
Volume 11, Issue 44
Winter 2020
February 2020
Pages 89-106

  • Receive Date 11 September 2019
  • Revise Date 15 December 2019
  • Accept Date 23 December 2019