Effect of six-week endurance exercise and hydroalcoholic extract of Urtica dioica on blood glucose level and necrotic cells of the hippocampal CA3 region of Wistar rats in type 1 diabetes model

Document Type : Research Paper

Authors
maryam keshvari 1
Masoud Rahmati 2
Rahim Mirnasuri 3
Farzaneh Chehelcheraghi 4
1 Ph.D. candidate of exercise physiology, Lorestan University
2 Associate Professor of Exercise Physiology, Lorestan University
3 Assistant Professor of Exercise Physiology, Lorestan University
4 Assistant Professor of Anatomical Sciences, Lorestan University Medical of Sciences
10.22089/spj.2020.8033.1969
Abstract
Due to the antioxidant effect of exercise and Urtica dioica(UD),the aim of this study was to evaluate the effects of these interventions on changes in blood glucose and necrotic cells in the CA3 region of the type 1 diabetic rat hippocampus.In this study,76 Wistar rats were divided into healthy(control,exercise,UD,UD-exercise) and diabetes (control, exercise,UD,UD-exercise)groups. Diabetes was induced by intraperitoneal injection of streptozotocin(45mg/kg) and 48 hours after injection, blood glucose levels above 300mg/dl were the criteria for confirmation of diabetes.After confirmation of diabetes, endurance exercise protocol with moderate-intensity(5days/week)and daily gavage of hydro alcoholic extract of Urtica dioica was performed at 50mg/kg for six weeks. 48 hours after the last session, the rats were sacrificed and the hippocampal tissue was extracted and fixed in 10% formalin for staining of crystal violet for necrotic cell count. Data were analyzed using ANOVA and Kruskal-Wallis tests using Bonferroni post hoc test at significance levelα=0.05. Results showed that six weeks of endurance exercise and consumption of UD extract significantly reduced blood glucose concentration in the diabetic-exercise(p=0.001),diabetes-UD(p=0.001)and diabetes-exercise-UD(p=0.001)groups compared with the diabetes-control group. In addition, there was a significant difference between the diabetes-UD and diabetes-exercise-UD(p=0.001)and diabetes-exercise and diabetes-exercise-UD(p=0.002) groups.The interaction between endurance exercise and consumption of UD extract significantly decreased the number of necrotic cells in the diabetes-exercise-UD group compared to the diabetic-control group (p=0.029).Finally, 6 weeks of endurance exercise and consumption of hydro alcoholic extract of UD can have protective effect on preventing necrosis of CA3 cells of diabetic rat hippocampus.
Keywords
Endurance Exercise
Urtica dioica
Necrotic
Hippocampus
Diabetes

Main Subjects

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  • Receive Date 27 October 2019
  • Revise Date 16 April 2020
  • Accept Date 04 May 2020