The Effect of 8 Weeks Aerobic Exercise on LXRa, PEPCK, and G6PC2 mRNA in Obese Prediabetic Mice

Document Type : Research Paper

Authors
Fatemeh Kazemi Nasab 1
Sayed Mohammad Marandi 2
Samaneh Shirkhani 3
Arezoo Sheikhanian Poor 3
Kamran Ghaedi 4
1 Assistant Professor of Exercise Physiology, Faculty of Human Sciences, University of Kashan, Kashan, Iran
2 Professor of Exercise Physiology, Faculty of Sport Sciences, University of Isfahan, Isfahan, Iran
3 M.Sc. of Exercise Physiology, Faculty of Sport Sciences, University of Isfahan, Isfahan, Iran
4 Professor of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran
10.22089/spj.2020.8717.2014
Abstract
The purpose of this study was to investigate the effect of 8 weeks aerobic exercise on the expression of LXRa, PEPCK and G6PC2 mRNA in the liver tissue of obese prediabetic mice. 18 male C57BL/6 mice were divided into two groups: 1. Mice fed a high-fat diet (prediabetic obese mice) (n=12), 2. Mice fed a standard diet (non-obese mice) (n= 6). Prediabetic conditions were confirmed by glucose tolerance test (GTT). Then, prediabetic mice were randomly divided into two groups: the high-fat diet-Exercised (HFD-Exe) and the high-fat diet-Sedentary (HFD-Sed). Exercise group were trained on a motor-driven treadmill at 21 m/min, 45 min/day, 5 days/week for 8 weeks. Mice were sacrificed 24 hours after last exercise session and blood was taken from the heart. The liver tissue was isolated and frozen to measure of LXRa and ABCA1 expression levels and was kept at -70 ̊C.Data indicated that the levels of liver LXRa and ABCA1 in trained mice were significantly higher than in untrained mice.The relative expression of key genes in gluconeogenesis, including G6PC2 and PEPCK in the liver of trained mice, was significantly reduced compared to untrained group. Also, aerobic exercise reduced steatosis and the liver enzymes in prediabetic mice.Following physical exercise, the expression of LXRa and ABCA1 mRNA expression in the liver of mice increases and protects mice from high-fat diet induced obesity and reduces the risk of NAFLD.
Keywords
Aerobic Exercise
High-Fat Diet
LXRa mRNA
Prediabetes
NAFLD

Main Subjects

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Sport Physiology
Volume 12, Issue 48
January 2021
Pages 17-38

  • Receive Date 10 April 2020
  • Revise Date 07 July 2020
  • Accept Date 09 August 2020