The comparison effects of a period of high intensity interval training and continues training on SIRT3 and antioxidant defense system of gastrocnemius muscle in female aged rats

Document Type : Research Paper

Authors
Ali Askarian 1
Abbas Ali Gaeini 2
Mohammad Reza Kordi 3
Siroos Choobine 4
1 Department of exercise physiology, Faculty of sport sciences, University of Tehran, Tehran, Iran
2 Professor
3 Department of exercise physiology, faculty of sport sciences, University of Tehran, Tehran Iran
4 Department of Exercise physiology, faculty of health sciences, University of Tehran, Tehran, Iran
10.22089/spj.2020.9044.2040
Abstract
the aim of this study was to comparison of 12 weeks of high intensity interval training and continues training on SIRT3 and superoxide dismutase (SOD) activity in gastrocnemius muscle of aged female Wistar rats. Methods: 15 female Wistar rats (age= 18 month, weight= 270-350 g) were chosen and divided into 3 groups; high intensity interval training (HIIT), Continues training (CT), and Control. After determining rat’s VO2max, progressive HIIT and CT protocols were designed with the intensity corresponds to 85-90% VO2max and 65-70 % VO2max, respectively and training duration in HIIT was approximately half of CT. 48 hours after the last training session, rats were sacrificed and their gastrocnemius muscle samples were saved for further analysis. Results: This study showed a significant difference of SIRT3 between HIIT, CT, and control group (p=0.001) (Control
Keywords
Training
Aging
Sirtuins
Free radicals
Antioxidant defense

Main Subjects

  1. Flatt T. A new definition of aging? Front 2012;3:148.
  2. Vina J, Borras C, Miquel J. Theories of ageing. IUBMB Life. 2007;59(4):249.
  3. Romano AD, Serviddio G, de Matthaeis A, Bellanti F, Vendemiale G. Oxidative stress and aging. Journal of Nephrology. 2009;23:S29-36.
  4. Kincaid B, Bossy-Wetzel E. Forever young: SIRT3 a shield against mitochondrial meltdown, aging, and neurodegeneration. Front Aging Neurosci. 2013;5:48.
  5. Michan S, Sinclair D. Sirtuins in mammals: insights into their biological function. Biochem J. 2007;404(1):1-13.
  6. Shi T, Wang F, Stieren E, Tong Q. SIRT3, a mitochondrial sirtuin deacetylase, regulates mitochondrial function and thermogenesis in brown adipocytes. J Biol Chem. 2005;280(14):13560-7.
  7. Ansari A, Rahman M, Saha SK, Saikot FK, Deep A, Kim KH. Function of the SIRT3 mitochondrial deacetylase in cellular physiology, cancer, and neurodegenerative disease. Aging cell. 2017;16(1):4-16.
  8. Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, et al. Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways. Proceedings of the National Academy of Sciences. 2013;110(16):6601-6.
  9. Vargas-Ortiz K, Perez-Vazque V, Diaz-Cisneros FJ, Figueroa A, Jiménez-Flores LM, Rodriguez-DelaRosa G, et al. Aerobic training increases expression levels of sirt3 and pgc-1α in skeletal muscle of overweight adolescents without change in caloric intake. Pediatr Exerc Sci. 2015;27(2): .
  10. McDonnell E, Peterson BS, Bomze HM, Hirschey MD. SIRT3 regulates progression and development of diseases of aging. Trends Endocrinol Metab. 2015;26(9):486-92.
  11. Lanza IR, Nair KS. Muscle mitochondrial changes with aging and exercise. The American Journal of Clinical Nutrition. 2009;89(1):467S-71S.
  12. Johnson ML, Irving BA, Lanza IR, Vendelbo MH, Konopka AR, Robinson MM, et al. Differential effect of endurance training on mitochondrial protein damage, degradation, and acetylation in the context of aging. The Journals of Gerontology Series A: Biological Sciences and Medical Sciences. 2015;70(11):1386-93.
  13. Menshikova EV, Ritov VB, Fairfull L, Ferrell RE, Kelley DE, Goodpaster BH. Effects of exercise on mitochondrial content and function in aging human skeletal muscle. The Journals of Gerontology Series A: Biological Sciences and Medical Sciences. 2006;61(6):534-40.
  14. Seldeen KL, Lasky G, Leiker MM, Pang M, Personius KE, Troen BR. High intensity interval training (HIIT) improves physical performance and frailty in aged mice. The Journals of Gerontology: Series A. 2017.
  15. MacInnis MJ, Zacharewicz E, Martin BJ, Haikalis ME, Skelly LE, Tarnopolsky MA, et al. Superior mitochondrial adaptations in human skeletal muscle after interval compared to continuous single‐leg cycling matched for total work. The Journal of Physiology. 2017;595(9):2955-68.
  16. Konopka AR, Suer MK, Wolff CA, Harber MP. Markers of human skeletal muscle mitochondrial biogenesis and quality control: effects of age and aerobic exercise training. Journals of Gerontology Series A: Biomedical Sciences and Medical Sciences. 2013;69(4):371-8.
  17. Fathi I, Nourshahi M, Haghparast A, Fallah HH. Effect Of eight-week aerobic continuous and high intensity interval training on levels of SIRT3 in skeletal muscle tissue of wistar rats. 2015. (In Persian).
  18. Edgett BA, Bonafiglia JT, Baechler BL, Quadrilatero J, Gurd BJ. The effect of acute and chronic sprint‐interval training on LRP 130, SIRT 3, and PGC‐1α expression in human skeletal muscle. Physiological Reports. 2016;4(17):e12879.
  19. Vargas-Ortiz K, Perez-Vazquez V, Diaz-Cisneros FJ, Figueroa A, Jiménez-Flores LM, Rodriguez-DelaRosa G, et al. Aerobic training increases expression levels of SIRT3 and PGC-1α in skeletal muscle of overweight adolescents without change in caloric intake. Pediatric Exercise Science. 2015;27(2):177-84.
  20. Andreollo NA, Santos EFd, Araújo MR, Lopes LR. Rat's age versus human's age: what is the relationship? ABCD Arquivos Brasileiros de Cirurgia Digestiva (São Paulo). 2012;25(1):49-51.
  21. Sengupta P. The laboratory rat: relating its age with human's. International Journal of Preventive Medicine. 2013;4(6):624-30.
  22. Høydal MA, Wisløff U, Kemi OJ, Ellingsen Ø. Running speed and maximal oxygen uptake in rats and mice: practical implications for exercise training. Eur J Cardiovasc Prev Rehabil. 2007;14(6):753-60.
  23. Seldeen KL, Lasky G, Leiker MM, Pang M, Personius KE, Troen BR. High intensity interval training improves physical performance and frailty in aged mice. The Journals of Gerontology: Series A. 2018;73(4):429-37.
  24. Tayebi SM, Siahkouhian M, Keshavarz M, Yousefi M. The Effects of High-Intensity Interval Training on Skeletal Muscle Morphological Changes and Denervation Gene Expression of Aged Rats. Montenegrin Journal of Sports Science and Medicine. 2019;8(2):39-45.
  25. Husain K. Physical conditioning modulates rat cardiac vascular endothelial growth factor gene expression in nitric oxide-deficient hypertension. Biochem Biophys Res Commun. 2004;320(4):1169-74.
  26. Lenk K, Schuler G, Adams V. Skeletal muscle wasting in cachexia and sarcopenia: molecular pathophysiology and impact of exercise training. Journal of Cachexia, Sarcopenia and Muscle. 2010;1(1):9-21.
  27. Picca A, Calvani R, Leeuwenburgh C, Coelho-Junior HJ, Bernabei R, Landi F, et al. Targeting mitochondrial quality control for treating sarcopenia: lessons from physical exercise. Expert opinion on Therapeutic Targets. 2019;23(2):153-60.
  28. Brown K, Xie S, Qiu X, Mohrin M, Shin J, Liu Y, et al. SIRT3 reverses aging-associated degeneration. Cell Reports. 2013;3(2):319-27.
  29. Palacios OM, Carmona JJ, Michan S, Chen KY, Manabe Y, Ward III JL, et al. Diet and exercise signals regulate SIRT3 and activate AMPK and PGC-1α in skeletal muscle. Aging (Albany NY). 2009;1(9):771-83.
  30. Vargas-Ortiz K, Pérez-Vázquez V, Figueroa A, Díaz FJ, Montaño-Ascencio PG, Macías-Cervantes MH. Aerobic training but no resistance training increases SIRT3 in skeletal muscle of sedentary obese male adolescents. European Journal of Sport Science. 2018;18(2):226-34.
  31. Herzig S, Shaw RJ. AMPK: guardian of metabolism and mitochondrial homeostasis. Nature Reviews Molecular Cell Biology. 2018;19(2):121-35.
  32. O’Neill HM, Holloway GP, Steinberg GR. AMPK regulation of fatty acid metabolism and mitochondrial biogenesis: implications for obesity. Molecular and Cellular Endocrinology. 2013;366(2):135-51.
  33. Finley LW, Haigis MC. Metabolic regulation by SIRT3: implications for tumorigenesis. Trends Mol Med. 2012;18(9):516-23.
  34. Vassilopoulos A, Pennington JD, Andresson T, Rees DM, Bosley AD, Fearnley IM, et al. SIRT3 deacetylates ATP synthase F1 complex proteins in response to nutrient-and exercise-induced stress. Antioxidants & Redox Signaling. 2014;21(4):551-64.
  35. Baba T, Shimizu T, Suzuki Y-i, Ogawara M, Isono K-i, Koseki H, et al. Estrogen, insulin, and dietary signals cooperatively regulate longevity signals to enhance resistance to oxidative stress in mice. Journal of Biological Chemistry. 2005;280(16):16417-26.
  36. Taguchi A, Wartschow LM, White MF. Brain IRS2 signaling coordinates life span and nutrient homeostasis. Science. 2007;317(5836):369-72.
  37. Qiu X, Brown K, Hirschey MD, Verdin E, Chen D. Calorie restriction reduces oxidative stress by SIRT3-mediated SOD2 activation. Cell Metabolism. 2010;12(6):662-7.
  38. Groussard C, Maillard F, Vazeille E, Barnich N, Sirvent P, Otero YF, et al. Tissue-specific oxidative stress modulation by exercise: a comparison between MICT and HIIT in an obese rat model. Oxidative Medicine and Cellular Longevity. 2019;2019:1-11.
  39. Gomez-Cabrera MC, Viña J, Ji LL. Role of redox signaling and inflammation in skeletal muscle adaptations to training. Antioxidants. 2016;5(4):48.
  40. Powers SK, Radak Z, Ji LL. Exercise‐induced oxidative stress: past, present and future. The Journal of Physiology. 2016;594(18):5081-92.
  41. Combes A, Dekerle J, Webborn N, Watt P, Bougault V, Daussin FN. Exercise‐induced metabolic fluctuations influence AMPK, p38‐MAPK and Ca MKII phosphorylation in human skeletal muscle. Physiological Reports. 2015;3(9):e12462.
  42. Bakhtiyari A, Gaeini A, Choubine S, Kordi M, Hedayati M. The comparison of the influence of 12-week high- intensity interval training and continuous moderate intensity training on PGC-1α and Tfam mitochondrial proteins expressions in gastrocnemius muscle of elderly rats. Journal of Animal Biology. 2020;11(4):11-20. (In Persian).
  43. Chistiakov DA, Sobenin IA, Revin VV, Orekhov AN, Bobryshev YV. Mitochondrial aging and age-related dysfunction of mitochondria. BioMed Research International. 2014;2014:1-7.
  44. Cui H, Kong Y, Zhang H. Oxidative stress, mitochondrial dysfunction, and aging. Journal of Signal Transduction. 2012;2012:646354.
  45. Toledo FG, Goodpaster BH. The role of weight loss and exercise in correcting skeletal muscle mitochondrial abnormalities in obesity, diabetes and aging. Molecular and Cellular Endocrinology. 2013;379(1-2):30-4.
  46. Joseph AM, Adhihetty PJ, Leeuwenburgh C. Beneficial effects of exercise on age‐related mitochondrial dysfunction and oxidative stress in skeletal muscle. The Journal of Physiology. 2016;594(18):5105-23.
  47. MacInnis MJ, Gibala MJ. Physiological adaptations to interval training and the role of exercise intensity. The Journal of Physiology. 2017;595(9):2915-30

 

Sport Physiology
Volume 14, Issue 55
December 2022
Pages 49-70

  • Receive Date 07 June 2020
  • Revise Date 04 October 2020
  • Accept Date 12 October 2020