Document Type : Research Paper
Authors
1 Assistant Professor, Department of Physiology, Faculty of Center of Physical Education, Isfahan University of Technology, Isfahan, Iran
2 Professor, Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan
Abstract
Objectives: Human athletic performance is an enormously complex multifactorial phenomenon, and is affected by numerous intrinsic (genetics) and extrinsic factors (e.g., training, nutrition). Total genotype score is a new model that was developed as a favorable polygenic profile in the elite athlete group, so the aim of this survey was the comparison of total genotype score of power/ strength responsible ACE, HIF1α and IGF1 polymorphisms of elite, amateur karate-kas vs. non-athletes.
Methods & Materials: This survey was done on 252 healthy Isfahanian subjects aged 27.2 ± 7.4 years including 124 males and 128 females sub-divided into elite (N= 80); amateur (N= 86) vs. non-athlete (N= 86 people with the same anthropometric characteristic by elite and amateur corps) groups. The amount of 5cc blood from brachiocephalic vein was taken in tubes including EDTA, then DNA was extracted using a modified salting-out method; polymorphisms were determined by PCR; and the restriction fragment lengths of the products were analyzed by electrophoretic separation, so TGS was calculated finally. Non–parametric Chi-Square and one-way ANOVA by using the SPSS software were used to assess the statistical differences. The level of significance was considered at P <0.05.
Results: Genotyping analyses indicated that in elite karate-ka, an increase in DD frequency was observed (63.0%) compared to non-athlete (41.0%) and amateur Karate-ka groups, significantly (14.7%) (χ2=14.430; P=0.011); but genotype distribution of HIF1α (χ2=2.746, P=0.60) and IGF1 (χ2=1.549, P=0.81) weren’t significantly different. In addition, the mean of TGS of elite karate-kas (69.85) was higher than amateur Karate-kas (64.40) and non-athlete (63.37), but it wasn’t significant (F=1.66, P=0.08).
Conclusions: Results have proved that the ACE is a sensitive polymorphism tool to differentiate elite from amateur karate-kas. Additionally, the use of TGS hadn’t been revealed in survey because karate has the multifactorial demands to for success likely; so, the genetic talent needs to be checked by a variety of polymorphisms in different geographical zones, too.
Keywords
- Kiss MAPDM, Böhme MTS, Mansoldo AC, Degaki E, Regazzini M. Performance and sports talent. Rev Paul Educ Fís. 2004;19(n. Esp):89-100
- Ahmetov II, Fedotovskaya ON. Current progress in sports genomics. Advances in Clinical Chemistry. 2015; 70:247-314.
- De Moor MH, Spector TD, Cherkas LF, Falchi M, Hottenga JJ, Boomsma DI, et al. Genome-wide linkage scan for athlete status in 700 British female DZ twin pairs. Twin Research and Human Genetics. 2007;10(6):812-20.
- Sayer AA, Syddall H, O'Dell SD, Chen XH, Briggs PJ, Briggs R, et al., Polymorphism of the IGF2 gene, birth weight and grip strength in adult men. Age and Ageing. 2002;31(6):468-70.
- Asoudeh A, Hooshmandfizabadi N, Jalal R. Extraction and purification of angiotensin converting enzyme from camel lung tissue and the effect of captopril inhibition on its activity. Isfahan Falavarjan Biological Sciences Conference, Isfahan; 2012.
- Fallah A, Fallahmohammadi Z, Behmanesh M, Gharakhanlou R, Alinaghizadeh M. The ACTN3 R577X polymorphism is associated with judo status in Iranian elite judo athletes. Journal of Applied Exercise Physiology, 2019;14(28):151-8. (In Persian).
- Khaledi N, Milani R, Arjmand S. Frequency of single nucleotide polymorphisms in athletic performance and athletic talent responsible genes of Iranian population and elite athletes, Bulletin of Application Sport Physiology. 2014; 21:101-16. )In Persian(.
- Salehi M, Ahmadpour A, Mohaddes M, Evaluation of actn3 polymorphism in Iranian elite athletes. Sport Physiology (Research on Sport Sciene). 2012;4(13):13– 21. )In Persian(.
- Hassan EA, Ali BM, Ali MM. Relationship Between maximum-intensity training with the gene expression of the female players of the Egypt national karate team. Training. 2011;1(13):13-7.
- Rankinen T, An P, Rice T, Sun G, Chagnon YC, Gagnon J, et al. Genomic scan for exercise blood pressure in the health, risk factors, exercise training and genetics (HERITAGE) family study. Hypertension. 2001;38(1):30-7
- Guilherme JP, Tritto AC, North KN, Lancha Junior AH, Artioli GG. Genetics and sport performance: current challenges and directions to the future. Revista Brasileira de Educação Física e Esporte. 2014;28(1):177-93.
- Möller S, Koczan D, Serrano-Fernandez P, Zettl UK, Thiesen HJ, Ibrahim SM. Selecting SNPs for association studies based on population frequencies: a novel interactive tool and its application to polygenic diseases. Silico Biology. 2004;4(4):417-27.
- Bray MS, Hagberg JM, Pe´russe L, Rankinen T, Roth SM, Wolfarth B, et al. The human gene map for performance and health-related fitness phenotypes: the 2006–2007 update. Med Sci Sports Exerc. 2009; 41:34–72.
- Gonzalez-Freire M, Santiago C, Verde Z, Lao JI, OIivan J, Gómez-Gallego F, et al. Unique among unique. Is it genetically determined? British Journal of Sports Medicine. 2009;43(4):307-9.
- Williams AG, Folland JP. Similarity of polygenic profiles limits the potential for elite human physical performance. The Journal of Physiology. 2008;586(1):113-21.
- Ruiz JR, Gómez‐Gallego F, Santiago C, González‐Freire M, Verde Z, Foster C, et al. Is there an optimum endurance polygenic profile? The Journal of Physiology. 2009;587(7):1527-34.
- Ruiz JR, Arteta D, Buxens A, Artieda M, Gómez-Gallego F, Santiago C, et al. Can we identify a power-oriented polygenic profile? J Appl Physiol. 2009; 108:561-6.
- Miyamoto-Mikami E, Murakami H, Tsuchie H, Takahashi H, Ohiwa N, Miyachi M, et al. Lack of association between genotype score and sprint/power performance in the Japanese population. Journal of Science and Medicine in Sport. 2016; http://dx.doi.org/10.1016/j.jsams.2016.06.005
- A. Karate in Olympic. Iran karate federation; 2020. Available at: www.Ikf .ir /htm/ - eng. Html.
- World Karate Federation. Kumite rules;. Available at: http://www.wkf.net/htm/
- Ibrahim AM. Planning principles for both educational and training programs in karate Manshaet Elmaref. Egypt: Alexandria; 1995.
- Timmons JA, Knudsen S, Rankinen T, Koch L, Sarzynski M, Jensen Th, et al. Using molecular classification to predict gains in maximal aerobic capacity following endurance exercise training in humans. J Appl Physiol. 2010; 108:1487-96.
- Mitchell J, Haskell W, Snell P, Van Camp S. Classification of sports. J Am CollCardiol. 2005;45(8):1364-7.
- Batavani MR, Batavani M, Batavani MA, Batavani R, Batavani N, Esteki Ouregani S. Analyses of pointing actions of top male competitors in Karate at world level and their physiological needs. First National Conference on New Findings of Sport Science Research in Health, Social Vitality, Entrepreneurship and Championship. Chamran Martyr of Ahwaz University, Ahvaz, Iran; 2016. (In Persian).
- Williams AG, Day SH, Folland JP, Gohlke P, Dhamrait S, Montgomery HE. Circulating angiotensin converting enzyme activity is correlated with muscle strength, Med Sci Sports Exerc. 2005; 37:944–8.
- Khodakarami H. The effect of angiotensin II on the differentiation of smooth muscle cells obtained from human embryonic stem cells [Master's thesis]. Tehran: Tarbiat Moaalem University; 2013.
- Batavani MR, Marandi SM, Ghaedi K, Esfarjani F. Comparison of genetic profile of single neucleotide polymorphisms in athletic performance responsible genes of male & female elite, amateur karate kaes & nonathletes [PhD dissertation]. Isfahan: University of Isfahan; 2017. (In Persian).
- Shokrzadeh M, Mohammadpour A. Evaluation of a modified salt-out method for DNA extraction from whole blood lymphocytes: a simple and economical method for gene polymorphism. Pharmaceutical and Biomedical Research. 2018;(2):28-32.
- Nazarov IB, Woods DR, Montgomery HE, Shneider OV, Kazakov VI, Tomilin NV, et al. The angiotensin converting enzyme I/D polymorphism in Russian athletes. Eur J Hum Genet. 2001; 9:797-801
- Woods D, Hickman M, Jamshidi Y, Brull D, Vassiliou V, Jones A, et al. Elite swimmers and the D allele of the ACE I/D polymorphism. Hum Genet. 2001; 108:230-2.
- Papadimitriou ID, Papadopoulos C, Kouvatsi A, Triantaphyllidis C. The ACE I/D polymorphism in elite Greek track and field athletes. J Sports Med Phys Fit. 2009; 49:459-63
- Boraita A, de la Rosa A, Heras M, Ana I, Canda A, Rabadán M, Hernánde M. Cardiovascular Adaptation, functional capacity, and angiotensin-converting Enzyme I/D polymorphism in elite athletes, Revista Española de Cardiología. 2010;63(7):810-9.
- Goldspink G. Gene expression in muscle in response to exercise. J Muscle Res Cell Motil. 2003; 24:121–6.
- Kothari ST, Chheda P, Chatterjee L, Das BR. Molecular analysis of genetic variation in angiotensin I-converting enzyme identifies no association with sporting ability: First report from Indian population. Indian J Hum Genet. 2012; 18:62-5
- Eynon N, Meckel Y, Alves AJ, Nemet D, Eliakim D. Is there an interaction between BDKRB2−9/+9 and GNB3 C825T polymorphisms and elite athletic performance? Scand J Med Sci Sports. 2011; doi:10.1111/j.1600- 0838.2010. 01261.x.
- Ahmetov II, Hakimullina AM, Lyubaeva EV, Vinogradova OL, Rogozkin VA. Effect of HIF1A gene polymorphism on human muscle performance. Bull Exp Biol Med. 2008; 146:351–3.
- Cieszczyk P, Sawczuk M, Maciejewska A, Ficek K, Eider J. Variation in peroxisome proliferator activated receptor α gene in elite combat athletes, Eur J Sport Sci. 2011;11:119–23.
- Zoll J, Ponsot E, Dufour S, Doutreleau S, Ventura-Clapier R, Vogt M, Hoppeler H, et al. Exercise training in normobaric hypoxia in endurance runners. III. Muscular adjustments of selected gene transcripts. J Appl Physiol. 2006; 100:1258-66.
- Ben-Zaken S, Meckel Y, Nemet D, Eliakim A. Can IGF-I polymorphism affect power and endurance athletic performance? Growth Hormone & IGF Research. 2013;23(5):175-8.
- Krych-Garsztka K, Mizgajska-Wiktor H, Goździcka-Józefiak A. An analysis of the regulatory region of the IGF1 gene in professional athletes in youth sports teams. Human Movement. 2011;12(3):216-22.
- Pieter W, Bercades L. Somatotypes of national Elite combative sport athlete. Brazilian Journal of Biomotiricity. 2009;3(1):21-30.
- He ZH, Hu Y, Li YC, Yvert Th, Santiago C, Gómez-Gallego F, et al . Are calcineurin genes associated with athletic status? A function, replication study. Med Sci Sports Exerc. 2011; 43:1433-40.
- De Bosscher V, De Knop P, Van Bottenburg M, Shibli S. A conceptual framework for analysing sports policy factors leading to international sporting success. European Sport Management Quarterly. 2006;6(2):185-215.
- Wang G, Mikami E, Chiu LL, Deason M, Fuku N, Miyachi M, et al. Association analysis of ACE and ACTN3 in elite Caucasian and East Asian swimmers. Med Sci Sports Exers. 2013; 45:892-900.
- Davoodi AR, Esmaili, MR, Kamkari, K. Evaluation of Talent search indicators in Iranian karate sports from the perspective of elite Iranian karate coaches and athletes [Master's thesis]. [Tehran]: Islamic Azad University, Central Tehran Branch, 2012.
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