Sport Physiology

Sport Physiology

The Effect of Short-term Eccentric Endurance Exercise on T-helper Cells Following Injection of Herpes Simplex Virus Type 1 Glycoprotein D DNA Vaccine

Document Type : Research Paper

Authors
Mohammad Taher Afshounpour 1
Mahdieh Molanouri Shamsi 2
Sara Soudi 3
Hoorieh Soleimanjahi 4
Reza Gharakhanlou 5
1 Department of Physical Education & Sport Sciences, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran
2 Tarbiat Modares University
3 Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
4 Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University; Tehran, Iran; Jala Ale Ahmad Exp. Tehran, I.R. Iran
5 Physical Education & Sport Sciences Dept., Faculty of Humanities, Tarbiat Modares University, Tehran, Iran; Jala Ale Ahmad Exp. Tehran
10.22089/spj.2021.9996.2109
Abstract
Exercise activities can act as an endogenous adjuvant, enhancing the efficiency of the host immune response after vaccination. However, the effect of repeated bouts of eccentric endurance exercise on cellular and humoral immune-related responses to the vaccine is unclear. The present study was performed to investigate the activation of type 1 and 2 (Th) helper lymphocytes following three bouts of endurance eccentric exercise as an adjuvant for DNA vaccination. Thirty-six female Balb-c mice (6-8 weeks, body mass mean=18g) were randomly divided into six groups: control, exercise, vaccine, PcDNA, exercise-vaccine and exercise-PcDNA (6 mice in each group). Different groups underwent DNA vaccine and PcDNA injection immediately after the last exercise, all stages of exercise and injections were repeated after 12 days according to the vaccine protocol, and 12 days after the second vaccine injection, spleen samples were collected. On-way variance analysis was used for statistical analysis and P<0.05 considered for significance. According to the results of the study, the ratio of Th1/Th2 after antigen stimulation was significantly different between the control group with vaccine and exercise-PcDNA (P <0.05). Also, the exercise-vaccine group was significantly different from all research groups (P <0.05). The results of the present study confirm the increase in the Th1/Th2 cell ratio following repeated bouts of eccentric endurance exercise. Stimulation of muscle damage through exercise before DNA vaccination may have been effective in invoking innate immune system cells and effectively stimulating acquired immunity.
Keywords
Endurance Exercise
Vaccine
Immune System
Adjuvant
Subjects
  1. Bartlett BL, Pellicane AJ, Tyring SK. Vaccine immunology. Dermatol Ther. 2009;22(2):104-9.
  2. Bolhassani A, Talebi S, Anvar A. Endogenous and exogenous natural adjuvants for vaccine development. Mini Rev Med Chem. 2017;17(15):1442-56.
  3. Pascoe AR, Fiatarone Singh MA, Edwards KM. The effects of exercise on vaccination responses: a review of chronic and acute exercise interventions in humans. Brain Behav Immun. 2014;39:33-41.
  4. Todryk SM. T Cell memory to vaccination. Vaccines (Basel). 2018;6(4):84.
  5. Gleeson M, Bishop N, Walsh N. Exercise immunology. London: Routledge; 2013.
  6. Long JE, Ring C, Bosch JA, Eves F, Drayson MT, Calver R, et al. A life-style physical activity intervention and the antibody response to pneumococcal vaccination in women. Psychosom Med. 2013;75(8):774-82.
  7. Gleeson M. Immune function in sport and exercise. Journal of Applied Physiology. 2007;103(2):693-9.
  8. Simpson RJ, Campbell JP, Gleeson M, Krüger K, Nieman DC, Pyne DB, et al. Can exercise affect immune function to increase susceptibility to infection? Exercise Immunology Review. 2020;26:8-22.
  9. Nielsen HG, Oktedalen O, Opstad PK, Lyberg T. Plasma cytokine profiles in long-term strenuous exercise. J Sports Med (Hindawi Publ Corp). 2016;2016:7186137.
  10. Peake JM. Exercise-induced alterations in neutrophil degranulation and respiratory burst activity: possible mechanisms of action. Exerc Immunol Rev. 2002;8:49-100.
  11. Parise G, McKinnell IW, Rudnicki MA. Muscle satellite cell and atypical myogenic progenitor response following exercise. Muscle Nerve. 2008;37(5):611-9.
  12. molanouri M, Jamali AJ, Mahdavi M. Adjuvant effect of short term endurance training on regulatory T cells in HSV-1 vaccine and its boosters. Journal of Sport Biosciences. 2018;10(1):77-85.
  13. Molanouri Shamsi M, Mahdavi M, Heydarzadeh E. Changes in interleukin-2 and interleukin-12 cytokines in spleen cells of balb-c mice after using an acute endurance exercise as an adjuvant for herpes simplex virus 1 vaccine. Sport Physiology. 2017;9(35):63-74.
  14. Giefing‐Kröll C, Berger P, Lepperdinger G, Grubeck‐Loebenstein B. How sex and age affect immune responses, susceptibility to infections, and response to vaccination. Aging Cell. 2015;14(3):309-21.
  15. Shamsi MM Najedi S, Hassan Z, Isanejad A, Mahdavi M. Short term exercise training enhances cell-mediated responses to HSV-1 vaccine in mice. Microbial Pathogenesis. 2017;110:457-63.
  16. Ghaemi A, Soleimanjahi H, Bamdad T, Soudi S, Arefeian E, Hashemi SM, et al. Induction of humoral and cellular immunity against latent HSV-1 infections by DNA immunization in BALB/c mice. Comp Immunol Microbiol Infect Dis. 2007;30(4):197-210.
  17. Edwards KM, Burns VE, Allen LM, McPhee JS, Bosch JA, Carroll D, et al. Eccentric exercise as an adjuvant to influenza vaccination in humans. Brain Behav Immun. 2007;21(2):209-17.
  18. Edwards KM, Burns VE, Reynolds T, Carroll D, Drayson M, Ring C. Acute stress exposure prior to influenza vaccination enhances antibody response in women. Brain Behav Immun. 2006;20(2):159-68.
  19. Paulsen G, Ramer Mikkelsen U, Raastad T, Peake JM. Leucocytes, cytokines and satellite cells: what role do they play in muscle damage and regeneration following eccentric exercise? Exercise Immunology Review. 2012;18:42-97.
  20. Thompson H, Scordilis S, Clarkson P, Lohrer W. A single bout of eccentric exercise increases HSP27 and HSC/HSP70 in human skeletal muscle. Acta Physiologica Scandinavica. 2001;171(2):187-93.
  21. Edwards KM, Campbell JP, Ring C, Drayson MT, Bosch JA, Downes C, et al. Exercise intensity does not influence the efficacy of eccentric exercise as a behavioural adjuvant to vaccination. Brain, Behavior, and Immunity. 2010;24(4):623-30.
  22. Rogers CJ, Zaharoff DA, Hance KW, Perkins SN, Hursting SD, Schlom J, et al. Exercise enhances vaccine-induced antigen-specific T cell responses. Vaccine. 2008;26(42):5407-15.
  23. Molanouri Shamsi M, Jamali A. Adjuvant effect of an acute moderate endurance exercise in herpes simplex virus 1 vaccination and its boosters on interleukin 10 cytokine and granzyme B in mice. Tehran University Medical Journal. 2018;76(2):96-102.
  24. Wang J, Song H, Tang X, Yang Y, Vieira V, Niu Y, et al. Effect of exercise training intensity on murine T‐regulatory cells and vaccination response. Scandinavian Journal of Medicine & Science in Sports. 2012;22(5):643-52.
  25. Clem AS. Fundamentals of vaccine immunology. Journal of Global Infectious Diseases. 2011;3(1):73.
  26. Smith TR, Patel A, Ramos S, Elwood D, Zhu X, Yan J, et al. Immunogenicity of a DNA vaccine candidate for COVID-19. Nature Communications. 2020;11(1):1-13.
  27. Jeyanathan M, Afkhami S, Smaill F, Miller MS, Lichty BD, Xing Z. Immunological considerations for COVID-19 vaccine strategies. Nature Reviews Immunology. 2020;20(10):615-32.
  28. Campbell JP, Edwards KM, Ring C, Drayson MT, Bosch JA, Inskip A, et al. The effects of vaccine timing on the efficacy of an acute eccentric exercise intervention on the immune response to an influenza vaccine in young adults. Brain, Behavior, and Immunity. 2010;24(2):236-42.
  29. Simpson RJ, Lowder TW, Spielmann G, Bigley AB, LaVoy EC, Kunz H. Exercise and the aging immune system. Ageing Research Reviews. 2012;11(3):404-20.
  30. Kiecolt-Glaser JK, Glaser R, Gravenstein S, Malarkey WB, Sheridan J. Chronic stress alters the immune response to influenza virus vaccine in older adults. Proceedings of the National Academy of Sciences. 1996;93(7):3043-7.
  31. Bohn-Goldbaum E, Pascoe A, Singh MF, Singh N, Kok J, Dwyer DE, et al. Acute exercise decreases vaccine reactions following influenza vaccination among older adults. Brain, Behavior, & Immunity-Health. 2020;1:100009.
Sport Physiology
Volume 15, Issue 57
Spring 2023
Pages 131-148

  • Receive Date 25 January 2021
  • Revise Date 05 May 2021
  • Accept Date 15 May 2021