Document Type : Research Paper
Authors
1 Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
2 Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
3 Department of Exercise Physiology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran
4 Department of Physical Education, Pardis Branch, Islamic Azad University, Pardis, Iran
Abstract
Objectives: The aim of this study was to investigate the effect of eight weeks of aerobic training and pumpkin seed supplementation on pulmonary oxidative stress indices of H2O2 poisoned rats.
Methods & Materials: 42 male Wistar rats were randomly divided into 7 groups: control, poisoned, poisoning+ 1 g pumpkin, poisoning+ 2 g pumpkin, poisoning+ aerobic exercise, poisoning+ 1 g pumpkin+ exercise, poisoning+ 2 g pumpkin+ exercise. Induction of oxidative stress by intraperitoneal injection of 0.1 mg/ kg of hydrogen peroxide for 3 days per week, receiving pumpkin seeds at 1 and 2 g per kg of body weight and exercise program including increasing running on a treadmill for 8 weeks (3 days Per week with a minimum speed of 8 meters per minute and a slope of 10 degrees). Specialized kits were used to measure cytochrome c, ATP, MDA and PAB indices and the data were analyzed by two-way analysis of variance at a significance level of 0.05.
Results: The results showed that H2O2 poisoning caused a significant decrease in ATP and significant increase in pulmonary cytochrome C, MDA and PAB (P <0.05). Pumpkin seed doses had no significant effect on MDA, cytochrome c and ATP concentrations (P> 0.05). On the other hand, receiving 2 g of pumpkin seeds caused a significant decrease in PAB concentration (P <0.05). Eight weeks of aerobic exercise had no significant effect on pulmonary MDA concentration (P> 0.05), but significantly increased ATP concentration and decreased cytochrome c and PAB concentrations (P <0.05). Interaction of aerobic exercise with pumpkin seed doses increased ATP and decreased concentrations of MDA, cytochrome c and PAB (P <0.05).
Conclusions: The single effects of pumpkin seeds seem to be dependent on tissue and dosage. Eight weeks of aerobic exercise is also a good antioxidant solution in hydrogen peroxide poisoning, depending on the factors. The combination of aerobic exercise and high doses of pumpkin seed supplement has additional effects on oxidative indices of lung tissue in rats poisoned with Hydrogen Peroxide.
Keywords
- Daneshmandi H, Bambaichi E, Rahnama N. Response of some oxidative stress
and inflammatory indicators to an acute exercise session following adaption
to 8 weeks running training. Sport Physiology. 2014;6(23):95-108.
DOI: journals.ssrc.ac.ir/article 32.html - Powers SK, Deminice R, Ozdemir M, Yoshihara T, Bomkamp MP, Hyatt H. Exercise-induced oxidative stress: Friend or foe? J Sport Health Sci. 2020;9(5):
415-25. DOI: 10.1016/j.jshs.2020.04.001 - Ariyanfar H, Matinhomaee H, Hosseini SA, Ghazalian F. The Effect of Endurance Training and Purslane (Portulaca oleracea) Seed Consumption on Cytochrome- C and Malondialdehyde in the Heart Tissue of Rats Poisoned with H2O2. Journal of Nutrition,Fasting and Health. 2020;8(2):80-6. DOI: 10.22038/jnfh.2020.44729.1237
- Poozesh jadidi R, Nourazar AR. Effect of 8 Weeks Aerobic Exercise Training with Chlorella Supplementation on Catalase and Superoxide Dismutase in the Heart of Diabetic Male Rats. Sport Physiology. 2018;10(38):181-96. DOI: 10.22089/spj.2018.4516.1605
- Tavana S, Amini S, Hakhamaneshi M, Andalibi P, Hajir M, Ardalan A, et al. Prooxidant-antioxidant balance in patients with phenylketonuria and its correlation to biochemical and hematological parameters. Journal of pediatric endocrinology & metabolism: JPEM. 2016;29. DOI: 10.1515/jpem-2015-0398.
- Gorzi A, Ekradi S. The Effect of Intake Duration of Curcumin Supplementation During Strenuous Endurance Training on GPX Activity and MDA Levels of Liver, Heart and Skeletal Muscle in Male Wistar Rats. Sport Physiology. 2020;12(46):139-56. DOI: 10.22089/spj.2020.8357.1989
- Ornatowski W, Lu Q, Yegambaram M, Garcia AE, Zemskov EA, Maltepe E, et al. Complex interplay between autophagy and oxidative stress in the development
of pulmonary disease. Redox Biology. 2020;36:101679.
DOI: 10.1016/j.redox.2020.101679 - Mahtabi Alamdari J, Matin Homaei H, Farzanegi P. The Effects of Vitamin D and Continuous Training on Apoptosis Markers in Aorta Tissue of Rats Exposed to Hydrogen Peroxide (H2O2). Sport Physiology. 2018;10(40):163-78.
DOI: 10.22089/spj.2019.7061.1877 - Mehrabani J, Ramezani N, Iranshahi F. The effect of interval aerobic training on malondialdehyde and total capacity antioxidant in sedentary women. Sport Physiology. 2014;6(22):81-94. DOI: elmnet.ir/article/720686-42701
- Sies H. Hydrogen peroxide as a central redox signaling molecule in
physiological oxidative stress: Oxidative eustress. Redox Biology. 2017;11.
DOI: 10.1016/j.redox.2016.12.035 - Ramzan R, Vogt S, Kadenbach B. Stress-mediated generation of deleterious ROS in healthy individuals - role of cytochrome c oxidase. Journal of Molecular Medicine. 2020;98(5):651-7. DOI: 10.1007/s00109-020-01905-y
- MacNee W. Pulmonary and Systemic Oxidant/Antioxidant Imbalance in Chronic Obstructive Pulmonary Disease. Proceedings of the American Thoracic Society. 2005;2:50-60. DOI: 10.1513/pats.200411-056SF
- Thirupathi A, Wang M, Lin J, Fekete G, Bíró I, Baker J, et al. Effect of Different Exercise Modalities on Oxidative Stress: A Systematic Review. BioMed Research International. 2021;2021. DOI: 10.1155/2021/1947928
- Gomes MJ, Pagan LU, Lima ARR, Reyes DRA, Martinez PF, Damatto FC, et al. Effects of aerobic and resistance exercise on cardiac remodelling and skeletal muscle oxidative stress of infarcted rats. 2020;24(9):5352-62. DOI: 10.1111/jcmm.15191
- Paes L, Lima D, Matsuura C, de Souza MdG, Cyrino F, Barbosa C, et al. Effects of moderate and high intensity isocaloric aerobic training upon microvascular reactivity and myocardial oxidative stress in rats. PloS one. 2020;15(2):e0218228-e.
DOI: 10.1371/journal.pone.0218228 - Frajtabar Z, Fathi R, Nasiri K, Ahmadi F. The Effect of Aerobic Exercise and Ethanol Consumption on Nrf2 Gene Expression in Heart Tissue and Some
Antioxidant Indices in Male Rat. Sport Physiology. 2021;13(49):65-88.
DOI: 10.22089/spj.2019.7664.1940 - Kurutas EB. The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state. Nutrition Journal. 2016;15(1):
DOI: 10.1186/s12937-016-0186-5 - Lestari B, Meiyanto E. A Review: The Emerging Nutraceutical Potential of Pumpkin Seeds. Indonesian Journal of Cancer Chemoprevention. 2018;9:92.
DOI: 10.14499/indonesianjcanchemoprev9iss2pp92-101
- Xanthopoulou MN, Nomikos T, Fragopoulou E, Antonopoulou S. Antioxidant and lipoxygenase inhibitory activities of pumpkin seed extracts. Food Research International. 2009;42(5):641-6. DOI: org/10.1016/j.foodres.2009.02.003
- shiri E, Rahnema M, Bigdeli M. The effect of pumpkin seed oil (Cucurbita Moschata) on the permeability of the blood-brain barrier and on brain edema in stroke animal model. Journal of North Khorasan University of Medical Sciences. 2016;8(2):
301-11.
DOI: 10.18869/acadpub.jnkums.8.2.301
- Shokohi Rad N, Bagherpour T, Nemati N, Hojati V. The effect of pumpkin seed and endurance training on oxidative stress factors and DNA damage.
Daneshvar Medicine: Basic and Clinical Research Journal. 2020;28(3):28-41.
DOI: magiran.com/p2164926 - Nazem F, Hokkamian E, Ranjbar K, Nazari A. The Effects of Aerobic Training on Renal Oxidative Stress in Myocardial Infarction Rats. Sport Physiology. 2017;9(34):79-94. DOI: 10.22089/spj.2017.1887.1241
- Shams Z, Azarbayjani MA, peeri M, Matin Homaee H. The effect of aerobic training and Vitamin Don GPx concentration and PABin lung tissue of rats exposed to Hydrogen Peroxide. Razi Journal of Medical Sciences. 2020;26(12):156-66.
DOI: rjms.iums.ac.ir/article-1-5876-en.html - Ransy C, Vaz C, Lombès A, Bouillaud F. Use of H2O2 to Cause Oxidative Stress, the Catalase Issue. International Journal of Molecular Sciences. 2020;21(23):9149. DOI: 10.3390/ijms21239149
- Shlyonsky V, Boom A, Mies F. Hydrogen Peroxide and Sodium Transport in the Lung and Kidney. BioMed Research International. 2016;2016:1-7.
DOI: 10.1155/2016/9512807 - Mohazab M, Matinhomaee H, Hosseini S, Rahmati-Ahmadabad S. The effects of endurance training and pumpkin seed consumption on oxidative stress and DNA damage markers in the cardiac muscle of rats poisoned with H2O2. 2019;6:12-9.
DOI: jbrms.medilam.ac.ir/article-1-453-en.html - Rouag M, Berrouague S, Djaber N, Khaldi T, Boumendjel M, Taibi F, et al. Pumpkin seed oil alleviates oxidative stress and liver damage induced by sodium nitrate in adult rats: biochemical and histological approach. African Health Sciences.
2020;20:413-25. DOI: 10.4314/ahs.v20i1.48 - Yeylaghi Ashrafi M, Dabidi Roshan V. Aerobic and Anaerobic Exercise of the Acute and Chronic and the Selected Markers of Oxidative Stress: A Systematic Review in Human and Animal Studies. Journal of Sabzevar University of Medical Sciences. 2016;22(Special Issue):1126-38. DOI: jsums.medsab.ac.ir/article_834.html
- Jabbari e, gholami m, nikbakht h, shakeri n, ghazaliyan f. Effect of aerobic training and L-carnitine supplementation on some apoptotic factors in diabetic rat liver. Razi Journal of Medical Sciences. 2019;26(7): 131-40. DOI: 10.18502/ijdo.v12i1.3635
- Balcı Ş, Pepe H, Guney S, Ç Ö, Revan S. Effects of Gender, Endurance Training and Acute Exhaustive Exercise on Oxidative Stress in the Heart and Skeletal Muscle of the Rat. Chin J Physiol. 2012;55. DOI: 10.4077/CJP.2012.BAA021
- Shetab-Boushehri S, Samavati-Sharif M, Ravasi A, Kordi M, Minaii B. Effect of oral iron supplementation and endurance training on cytochrome C oxidase activity in rat soleus muscle. International Journal of Pharmacy and Pharmaceutical Sciences. 2010;2:33-5. DOI: eprints.iums.ac.ir/id/eprint/21291
- Gorzi A, Ekradi S, Rahmani A. The Effect of 8 Weeks of Sprint Interval Training on Oxidative and Antioxidative Capacity of Heart, Liver and Skeletal Muscle in Male Wistar Rats. Sport Physiology. 2018;10(37): 123-38. DOI: 10.22089/spj.2018.1155
- Bangsbo J, Krustrup P, González-Alonso J, Saltin B. ATP production and
efficiency of human skeletal muscle during intense exercise. American
journal of physiology Endocrinology and metabolism. 2001; 280:E956-64.
DOI: 10.1152/ajpendo.2001.280.6.E956 - Houghton CR, Hawkins RA, Williamson DH, Krebs HA. The effects of physical training on the metabolic response to short-term severe exercise in the rat. Biochemical Journal. 1971;124(5):57P-P. DOI: 10.1042/bj1240057pa
- Ghanbari-Niaki A, Kraemer R, Abednazari H. Time-course alterations of plasma and soleus agouti-related peptide and relationship to ATP, glycogen, cortisol, and insulin concentrations following treadmill training programs in male rats. Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme. 2011;43 2:112-6. DOI: 10.1055/s-0030-1267998
- Ramak P, Mahboubi M. The beneficial effects of Pumpkin (Cucurbita pepo L.) seed oil for health condition of men. Food Reviews International. 2019;35(2):166-76.
DOI: 10.1080/87559129.2018.1482496
- Abou Seif HS. Ameliorative effect of pumpkin oil (Cucurbita pepo L.) against alcohol-induced hepatotoxicity and oxidative stress in albino rats. Beni-Suef University Journal of Basic and Applied Sciences. 2014;3(3):178-85.
DOI: org/10.1016/j.bjbas.2014.08.001 - Pilch W, Szygula Z, Tyka A, Pałka T, Tyka A, Cisoń T, et al. Disturbances in Pro-Oxidant-Antioxidant Balance after Passive Body Overheating and after Exercise in Elevated Ambient Temperatures in Athletes and Untrained Men. PloS one. 2014;9:e85320. DOI: 10.1371/journal.pone.0085320
- Roh H-T, So W-Y. The effects of aerobic exercise training on oxidant–antioxidant balance, neurotrophic factor levels, and blood–brain barrier function in obese and non-obese men. Journal of Sport and Health Science. 2017;6(4):447-53.
DOI: 10.1016/j.jshs.2016.07.006
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