The purpose of this study was to investigate effects of high intensity interval training (HIIT) and curcumin supplementation on cardiomyocyte miR-208, miR-499 and HSP60 expression level in rat model of isoproterenol induced acute myocardial infraction (AMI). 40 male rats (200-250 gr, age: 16 weeks old) were randomized into four groups of Training, Curcumin, Concomitant (HIIT+ Curcumin) and Control following intraperitoneal injection of isoproterenol (100 mg/kg) for two consecutive days to induce myocardial infarction. HIIT were done for eight weeks, 5 sessions a week for 60 min. Each session was included to 10 intervals (each interval for 4 min) of running at 85-90% of v VO2 peak with 2 min of active recovery at 50 to 60% of v VO2 peak. Curcumin was administrated through oral gavage 15 mg/kg a day. Expression levels of miR-208, miR-499 and HSP60 genes was evaluated using Real-Time PCR method. All three interventions of training, curcumin and their combination (HIIT+Curcumin) significantly elevated cardiac miR-499, HSP60 expression level as well as Vo2max improvement; meanwhile, miR-208 expression was reduced (p=0.001 under any circumstances). Moreover, the expression levels of miR-208 in the concomitant group were significantly lower than HHI (p=0.03) or curcumin (p=0.049) either. All three interventions are likely associated with alleviation of MI complications e.g. oxidative stress, ischemia/reperfusion injury and tissue remodeling, through modifications in microRNAs expression level. Moreover, a further reduction in miR-208 expression level in the concomitant group could imply on a lesser mortality rate or recurrent AMIs as well as a better cardiac structural remodeling following to infraction; emphasizing on better results with curcumin plus HIIT co-prescription. However, more investigations are warranted because of no straight measurement of many factors and also limitations in this study.