The Effect of Four Weeks of Aerobic Training with Naringenin Supplementation on Hippocampal Adiponectin Levels and Memory Impairment in Rats with Alzheimer’s Disease Induced by Amyloid beta Injection

Document Type : Research Paper

Authors

1 Master of Sport Physiology,, Department of Exercise Physiology, Faculty of Sport Science, Shahid Rajaee Teacher Training University, Tehran, Iran

2 Assistant Professor of Sport Physiology, Department of Exercise Physiology, Faculty of Sport Science, Shahid Rajaee Teacher Training University, Tehran, Iran

3 Assistant Professor of Physiology, Department of Physiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Abstract

Alzheimer’s disease is the most common form of dementia. Despite the lack of definitive treatment for this disease, exercise and flavonoid use have been suggested as possible non-pharmacological approaches to reduce the risk of Alzheimer’s disease. Therefore, the purpose of this study was to evaluate the effect of four weeks of aerobic exercise with Naringenin supplementation on hippocampal adiponectin levels and memory impairment in rats  with Alzheimer’s disease, induced by Aβ1-42 injection. For this purpose, 32 eight-week-old male rats (weight 232±26 gr) were randomly divided into four groups: Alzheimer’s disease (AD), AD + aerobic exercise group (ADET), AD + Naringenin supplementation group (AND), and AD + aerobic exercise + Naringenin supplementation group (ADETN). AD was induced by injection of Aβ1-42 into the hippocampus. 10 days after surgery, aerobic exercise interventions (five times a week for four weeks) and / or Naringenin supplementation (80 mg.kg-1.day-1) were started. Then, the animals were subjected to behavioral tests. 48 hours after last session of exercise training, animals were anesthetized and hippocampus were removed. Western Blotting analysis was used to measure Adiponectin protein levels. The results showed that learning and spatial memory performance in ADET, ADN and ADETN groups improved significantly compared to AD group (p < 0.001). Also hippocampal adiponectin levels in ADET, ADN and ADETN groups increased significantly compared to AD group (p < 0.001). In addition, rats in the ADETN group showed better spatial memory function and higher hippocampal adiponectin levels compared to the ADET and ADN groups (p < 0.05). In general, our data suggested that both aerobic exercise and Naringenin supplementation improved learning and spatial memory in rats with Alzheimer’s disease, possibly by modulating hippocampal adiponectin levels. In addition, the combination of these factors had more effect on improving memory.

Keywords

References

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History

  • Receive Date: 05 January 2021
  • Revise Date: 21 November 2021
  • Accept Date: 27 November 2021

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