The effect of endurance training on muscle glycogen storage in pre-diabetic C57BL/6 mice

Document Type : Research Paper

Authors
Seyed gholamhoseyn Rahimi 1
seyed mohammad marandi 2
Mohammad Hossein Nasr-Esfahani 3
1 Department of Exercise Physiology, Faculty of Sports Sciences, University of Isfahan, Isfahan, Iran
2 Professor of Exercise Physiology, Faculty of Sports Sciences, University of Isfahan, Isfahan, Iran
3 Professor of Biology, Biotechnology Research Institute, Royan Research Institute, Iran
10.22089/spj.2022.12736.2189
Abstract
Pre-diabetic stressful conditions are status before the onset of type 2 diabetes, in which the blood sugar level is higher than normal and below the diagnostic criteria for type 2 diabetes. Therefore, the purpose of this study was to investigate the effect of endurance training on muscle glycogen storage in pre-diabetic mice. For this purpose, Fifteen male C57BL / 6 mice were randomly divided into two groups: mice fed a normal diet (ND, n= 5) and a high-fat diet (HFD, n=10) and then fed for 12 weeks. After diagnosing prediabetes induction by diagnostic tests in the HFD group, this group was into two groups: the pre-diabetic group (PD) and pre-diabetic mice along with endurance training (PD-Ex). The results showed a significant decrease in blood glucose levels and insulin resistance of HFD-Ex group compared to PD. The PAS technique showed an observational decrease in glycogen storage in the HFD group compared to the ND group, while endurance training increased it in the skeletal muscle of mice. The relative gene expression of the factors involved in glycogen storage such as AKT, PI3K, Gys1, and GSK3 as well as the gene LRRC8A showed significant changes (p <0.05) between the ND group compared to the HFD group, showing the glycogen storage signaling pathway is disturbed. However, endurance training (PD-Ex) has resulted in a significant improvement in glycogen synthase gene levels. In general, Endurance training seems to improve the negative effects of pre-diabetic conditions due to a high-fat diet.
Keywords
Prediabetes
Glycogen synthase
High fat diet
endurance training
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Sport Physiology
Volume 14, Issue 56
February 2023
Pages 107-146

  • Receive Date 10 May 2022
  • Revise Date 25 August 2022
  • Accept Date 27 August 2022