Comparison of NRF2 gene polymorphism rs12594956 in elite and amateur karate-kas vs. non-athletes

Document Type : Research Paper

Authors

1 Assistant Professor, Center of Physical Education, Isfahan University of Technology, Isfahan 84156-83111, Iran

2 2Professor of Sports Physiology Department, Faculty of Physical Education and Sports Sciences, Isfahan University, Isfahan, Iran

3 3Assistant Professor, Department of Sports Biomechanics, Sport Sciences Research Institute, Tehran, Iran

4 Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran/Department of Cellular Biotechnology, Cell Sciences Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.

Abstract

The genetic status of athletes plays a significant role in their success. Considering the importance of NRF2 gene polymorphism in aerobic capacity as one of the success factors of athletes, the purpose of this research Comparison of NRF2 gene polymorphism rs12594956 in elite and amateur karate-kas vs. non-athletes. Blood samples were collected in tubes, after DNA extraction, PCR for determining the type of A/C polymorphisms by preparation of primers, samples were electrophoresed on 1% agarose gel using RFLP method via MfeI enzyme. To analyze the data, simple ANOVA, chi-square and logistic regression used in SPSS version 21 software. The significance level was also determined at p<0.05. Findings: The results showed that there was significant within-group difference in distribution of A/C in amateur groups (χ2=8.667, P=0.013) and non-athletes (χ2=6.494, P=0.039). While this distribution did not show a significant difference between all 3 groups (χ2=3.121, P=0.528). Also, in elite athletes, compared to non-athletes, frequency of A/A is higher than C/C: with a coefficient of 1.049, and frequency of A/C is higher than C/C with a coefficient of 0.918 was less. In the case of amateur athletes, the frequency of A/A compared to C/C with a factor of 1.701 times that of non-athletes and the frequency of A/C compared to C/C with a factor of 1.312 times It was more in amateur athletes than non-athletes. Conclusion: The results of the present research show the high previlance of genotypes related to the lower function of the NRF2 gene for the aerobic energy supply system.

Keywords

Main Subjects

References

  1. Babic M, Kezic A, Cular D. The future of genetic testing in taekwondo: opportunities and challenges. Physical Activity Review; 2023;10(2):21-33.
  2. Khaledi N, Milani R, Arjmand S. Frequency of single nucleotide polymorphisms in athletic performance and athletic talent responsible genes of Iranian population and elite athletes. Bulletin of Application Sport Physiology. 2014;21:101-16. (In Persian).
  3. Salehi M, Ahmadpour A, Mohaddes M, Evaluation of actn3 polymorphism in Iranian elite athletes. Sport Physiology (Research on Sport Sciene). 2012;4(13):13–21.
    (In Persian).
  4. MacArthur DG, North KN. Genes and human elite athletic performance. Human Genetics. 2005;116(5):331-9.
  5. Ahmetov II, Rogozkin VA. Genes, athlete status and training–an overview. Med Sport Sci. 2009;54(4):43-71.
  6. Ahmetov II, Fedotovskaya ON. Current progress in sports genomics. Advances in Clinical Chemistry. 2015;70:247-314.
  7. Hassan EA, Ali BM, Ali MM. Relationship Between maximum-intensity training with the gene expression of the female players of the Egypt national karate team. Training. 2011;1(13):13-7.
  8. Rankinen, T, An P Rice, T, Sun G, Chagnon. C, Gagnon J, Bouchard C. Genomic scan for exercise blood pressure in the health, risk factors, exercise training and genetics (HERITAGE) family study. Hypertension .2001;38(1):30-7.
  9. Brewster UC, Perazella MA. The renin-angiotensin-aldosterone system and the kidney: effects on kidney disease. Am J Med. 2004;116(3):263–72.
  10. Batavani MR, Ghaedi K. Comparison of total genotype score (TGS) of power/strength responsible ACE, HIF1α and IGF1 polymorphisms of elite, amateur karate-kas vs. non-athletes. Sport Physiology. 2022;14(53):149-76. (In Persian).
  11. Imamura H, Yoshimura Y, Uchida K, Nishimura S, Nakazaw AT. Maximal oxygen uptake, body composition and strength of highly competive and novice karate practitioners. Journal of Physiological Anthropologies. 1998;17(5):215-8.
  12. Boroushak N, Anbarian M. A comparison of time to peak torque and acceleration time in elite karate athletes. Journal of Paramedical Science and Rehabilitation.
  13. Nunan D. Development of a sports specific aerobic capacity test for karate - a pilot study. J Sports Sci Med. 2006;1(5):47-53.
  14. Doria C, Veicsteinas A, Limonta E, Maggioni MA, Aschieri P, Eusebi F, et al. Energetics of karate (kata and kumite techniques) in top-level athletes. European Journal of Applied Physiology.
  15. Cid-Calfucura I, Herrera-Valenzuela T, Franchini E, Falco C, Alvial-Moscoso J, Pardo-Tamayo C, et al. Effects of strength training on physical fitness of olympic combat sports athletes: a systematic review. International Journal of Environmental Research and Public Health. 2023;20(4):16-35. https://pubmed.ncbi.nlm.nih.gov/36834211/
  16. Nikoukheslat S, Vakili J, Fatollahi S. Effect of one month of common trainings on physical fitness, body composition and performance of kata international champions. Sport Physiology.2019;40(10):33-50. (In Persian). https://spj.ssrc.ac.ir/article_1364.html
  17. Boraita A, de la Rosa A, Heras M, Ana I, Canda A, Rabadán M, and Hernánde M. Cardiovascular adaptation, functional capacity, and angiotensin-converting enzyme I/D polymorphism in elite athletes. Revista Española de Cardiología (English Edition). 2010;63(7):810-19. https://pubmed.ncbi.nlm.nih.gov/20609315/
  18. Eynon N, Ruiz JR, Bishop DJ, Santiago C, Gómez-Gallego F, Lucia A, et al. The rs12594956 polymorphism in the NRF-2 gene is associated with top-level Spanish athlete's performance status. Journal of Science and Medicine in Sport. 2013;16(2):135-9.
  19. Maciejewska-Karłowska A, Leońska-Duniec A, Cięszczyk P, Sawczuk M, Eider J, Ficek K, et al. The GABPB1 gene A/G polymorphism in Polish rowers. Journal of Human Kinetics. 2012;31:115-20.
  20. Babić M, Kezić A, Cular D. The future of genetic testing in taekwondo: opportunities and challenges. Physical Activity Review. 2023;10(2):21-33.
  21. Eynon N, Sagiv M, Meckel Y, Duarte JA, Alves AJ, Yamin C, et al. NRF2 intron 3 A/G polymorphism is associated with ndurance athletes’ status. Journal of Applied Physiology. 2010;107(1):76-9.
  22. Eynon N, Sagiv M, Meckel Y, Duarte JA, Alves AJ, Yamin CJ. NRF2 intron 3 A/G polymorphism is associated with ndurance athletes’ status. Journal of Applied Physiology. 2009;107(1):76-9.
  23. Guilherme JPL, Souza-Junior TP, Lancha Junior AH. Association study of performance-related polymorphisms in Brazilian combat-sport athletes highlights variants in the GABPB1 gene. Physiol Genomics. 2021;53(2):47-50.
Sport Physiology
Volume 15, Issue 57
April 2023
Pages 67-86

Files

History

  • Receive Date: 15 April 2023
  • Revise Date: 22 July 2023
  • Accept Date: 05 August 2023

Share

How to cite

Statistics