Document Type : Research Paper
Author
Department of Exercise physiology, Facuity of sport Science and Heaith, University of Tehran
Abstract
Objective: Heart failure is a clinical syndrome that known as end stage of cardiovascular disease. Oxidative stress is an important pathogenesis factor in development of heart failure. The purpose of this study was investigation of high-intensity interval training on myocardial protein expression of SIRT3, FOXO3a, SOD2 and cardiac function.
Methods and materials: 24 male Wistar rats randomly divided into three groups: health control, heart failure- control and heart failure-high-intensity interval training. Heart failure was induced by 130 mg/kg isoproterenol for 4 days. Exercise training including 5 intervals, 4 minutes at 85-90% maximum oxygen consumption separated with 2 minutes at 50-60% maximum oxygen consumption. 48 h after last session training tissue was collected and protein expression was done by western blotting. Data analysis performed using by SPSS and one-way ANOVA and Tukey post hoc.
Results: The results indicated that SIRT3 (P< 0.001), FOXO3a (P < 0.05) and SOD2 (P< 0.01) significantly reduced after exercise intervention. Additionally, interstitial fibrosis decreases after 8 weeks high intensity interval training concomitant with increase in ejection fraction, fractional shortening and decrease of left ventricular internal systolic (P< 0,001).
Conclusions: High- intensity interval training decreases oxidative stress and improves cardiac function via SIRT3/FOXO3a/SOD2 signaling pathway in rats with heart failure.
Keywords
Main Subjects
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