Document Type : Research Paper
Authors
- Mohammad Hassan Olamazadeh 1
- fahimeh Esfarjani 1
- Sayed Mohammad Marandi 2
- saeed zamani 3
- Bahman Rashidi 3
1 Department of Exercise Physiology, Faculty of Sport Science, University of Isfahan, Isfahan, Iran
2 Department of Exercise Physiology, Faculty of Sport Sciences, University of Isfahan, Isfahan, Iran
3 Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract
Objectives: Angiogenesis plays a vital role in heart function. Irisin is a possible mediator of exercise effects. The aim of the present study was to investigate the serum irisin level and the expression of vascular endothelial growth factor gene and protein in the heart tissue of NMRI mice after resistance training and irisin injection.
Materials and methods: 21 mice (NMRI, 5 weeks old, 18±2 grams) were randomly divided into three groups: control, exercise (resistance training) and irisin. The exercise protocol was implemented for eight weeks. A weight equal to 30% of the mice's weight was attached to their tails, and they climbed a ladder with a height of one meter. The weight gradually increased to twice the weight of the mouse. The irisin group received irisin at a dose of 100 μg/kg/week intraperitoneally for eight weeks. VEGF gene expression in the heart was evaluated using the real-time PCR method, VEGF protein was assessed by immunohistochemistry and western blot, and irisin serum concentration was measured by ELISA.
Findings: In comparison to the control group, the other study groups showed increased expression of VEGF gene and protein, as well as increased serum levels of irisin (P < 0.05). Pearson correlation coefficient demonstrated a significant positive relationship between these parameters within each group (P < 0.05, r > 0).
Conclusion: Irisin probably mediates the relationship between resistance training and angiogenesis in heart tissue.
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