Sport Physiology

Sport Physiology

Comparison of the Effects of a Single-Session and Multi-Session Iso-Volume High-Intensity Interval Training on Changes in Selected Physical Fitness Components in Professional Athlete

Document Type : Research Paper

Authors
1 Professor of Sport Physiology, Kharazmi University
2 Kharazmi University, Faculty of Physical Education and Sport Sciences, Department of Exercise Physiology
3 Arak University, Faculty of Physical Education and Sport Sciences, Department of Exercise Physiology
Abstract
**Objective:** This study compared the effects of four weeks of volume-matched single-bout and multi-bout high-intensity interval training (HIIT) on physical fitness in professional athletes, as the impact of multi-bout training in elite populations remains underexplored.



**Methods:** Twenty handball and basketball players (mean age: 21 years) were randomly assigned to single-bout (n = 10) or multi-bout (n = 10) HIIT groups. Training was performed three times per week for four weeks. The single-bout group completed three 6-minute HIIT blocks in one session, while the multi-bout group performed the same workload across three daily sessions separated by 4-hour intervals. Aerobic capacity, sprint performance, agility, muscular strength and endurance, vertical jump, and Hooper Index were assessed pre- and post-intervention using repeated-measures ANOVA.



**Results:** Both groups showed significant improvements in aerobic capacity, muscular strength, and muscular endurance. No significant changes were observed in sprint performance, agility, or vertical jump. The multi-bout group showed more favorable, though non-significant, improvements in Hooper Index scores, including reduced fatigue, stress, and muscle soreness, and better sleep quality.



**Conclusion:** Both HIIT protocols effectively improved key fitness variables, while multi-bout training may provide additional benefits in recovery and training load management for professional athletes.
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Articles in Press, Accepted Manuscript
Available Online from 23 September 2026

  • Receive Date 23 June 2026
  • Revise Date 21 September 2026
  • Accept Date 23 September 2026