Document Type : Research Paper

Authors

urmia

Abstract

 The purpose of this study was to investigate the effects of glutamine supplementation and judo preparatory training on positive and negative acute phase proteins in young Judoists. Accordingly, in a Quasi-experimental, double-blinded study, 48 young Judo (age: 21.34±5.13 years, height: 173.54±3.7 cm, weight: 70.56±6.14 kg) were randomly and easily- accessible selected, and randomly divided into 4 groups (n=12 for each group): exercise-supplementation, exercise placebo, supplementation, and control. Subjects in the supplementation group and placebo (maltodextrin) in prior and post of each session consumed 0.1 g/kg Bw supplement or placebo in Combined with 300 ml of water. The exercise program comprised Judo fitness training for 14 days and 5 sessions per week. Resulting data were analyzed by dependent t-tests and two-way ANOVA test at 0.5alpha error levels (P<0.05). statistical test results indicated that not only reduction in Haptoglobin was influenced by the separate effects of supplements and exercise, but combined intervention on both exercise and supplements had a synergistic effect on the reducing the concentrations of this acute phase protein. In addition, the effect of exercise alone and exercise accompanied by glutamine led to significant increase in albumin level in the post-test compared to baseline. While the glutamine supplementation alone, only led to reduces serum haptoglobin levels. Serum CRP levels during exercise in study groups remained unchanged. In summary, research result show that two two-week judos preparatory training can cause mild and positive acute phase response, and two weeks Glutamine Supplementation Lead to increased protein synthesis and strengthens the immune system in young judoists.

Keywords

1) Michael G. Immune function in sport and exercise. Journal of Applied Physiology. 2007; 103(2): 694-7.
2) عسگرپور ثریا، نظرعلی پروانه، باقرصادرنانی لیلا. بررسی ارتباط بین غلظت IL-6 و بیش‌تمرینی پس از یک دوره تمرین کوتاه‌مدت شدید در زنان ورزشکار نخبه. نشریۀ فیزیولوژی ورزشی. 1391؛ 9(16): 3ـ42.
3) Haskell A, Nadel E R, Stachenfeld N S, Nagashima K, Mack G W. Trans capillary escape rate of albumin in humans during exercise-induced hypervolemia. J Appl Physiol. 1997; 83(2): 407-13.
4) Semple S J, Smith L, Mckune A, Mokgethawa B, SanJuan A, Lucia A, et al. Serum concentration of C-reactive protein, antitrypsin, and complement (c3, c4, c1 esterase inhibitor) before and during the Vuelta Espana. Br J Sports Med. 2006; 40(2): 124–7.
5) Oh M K, Park H J, Kim N H, Park S J, Park I Y, Kim I S. Hypoxia-inducible factor-1alpha enhances haptoglobin gene expression by improving binding of STAT3 to the promoter. J Biol Chem. 2011; 286(11): 8857-65.
6) Owens J, Spitler D L, Frey M A. Hemolysis in runners as evidenced by low serum haptoglobin: Implications for preflight monitoring of astronauts. Florida: The bionetics corporation 8iomedical research laboratory kenedy space center; 1987. P. 12-5.
7) Medina R, Corona I, Barajas S, Díaz M, Durán G. Albumin antioxidant response to stress in diabetic nephropathy progression. PLoS One. 2014; 9(9): 2-5.
8) Daniella M, Rex M, Tyrrel L, Dylan T. Acute moderate-intensity exercise in middle-aged men has neither anti-nor pro inflammatory effect. J Appl Physiol. 2008; 105(1): 260-5.
9) Poortmans, J R. Serum protein determination during short exhaustive physical activity. Journal of Applied Physiology. 1971; 30(2): 190-2.
10) Walsh N P, Blannin A K, Robson P J, Gleeson M. Glutamine, exercise and immune function. Links and possible mechanisms. Sports Medicine. 1998; 26(3): 177-91.
11) Gleeson M. Dosing and efficacy of Glutamine supplementation in human exercise and sport training. J Nutr. 2008; 138(10): 2045-9.
12) Williams R, Olivi S, Li C, Storm M, Cremer L, Mackert P, et al. Oral Glutamine supplementation decreases resting energy expenditure in children and adolescents with sickle cell Anemia. J Pediatr Hematol Oncol. 2004; 26(10): 619-25.
13) Francesco A, Gianni B. Effect of physical activity on glutamine metabolism. Curr Opin Clin Nutr Metab Care. 2010; 13(1):58-64.
14) حسینی یعقوب، نعمتی غلامرضا، میرزایی بهمن. اثر یک دوره تمرین قدرتی با 2 الگوی باردهی متفاوت (هرمی دوگانه و هرمی مسطح) بر قدرت، استقامت، حجم عضلانی و توان بی‌هوازی در جودوکاران جوان. نشریۀ فیزیولوژی ورزشی. 1393؛ 6(23): 1ـ30.
15) Wasserman K, Hansen J, Sue D Y, Casaburi R, Whipp B J. Principles of exercise testing and interpretation. University of California, Los Angeles, USA. Can J Cardiol. 2007 Mar 15; 23(4): 274.
16) Krieger J W, Crowe M, Blank S E. Chronic glutamine supplementation increases nasal but not salivary IgA during 9 days of interval training. J Appl Physiol. 2004; 97(2): 585-91.
17) Castell L M, Poortmans J R, Leclercq R, Brasseur M, Duchateau J, Newsholme E A. Some aspects of the acute phase response after marathon race, and the effect of glutamine supplementation. Eur J Appl Physiol Occup Physiol. 1997; 75(1): 47-53.
18) Teleford R D, Sly G J, Hahn A G, Cunningham R B, Bryant C, Smith J A. Foot strike is the major cause of hemolysis during running. J Appl Physiol. 2003; 94(1): 38-42.
19) Duca L, Ponte A, Cozzi M, Carbone A, Pomati M, Nava I, et al. Changes in erythropoiesis, iron metabolism and oxidative stress after half- marathon. Intern Emerg Med. 2006; 1(1): 30-4.
20) Tsukanaka A U, Memura Y, Ishiko T, Matui N. changes in serum Haptoglobin concentration by Tread mill-running and ergometer-cycling. Exerc Sports Physiol. 2000; 6(1): 1-6.
21) Makoto Y, Takashi U, Ippei T, Masashi M, Norio S, Sen S, et al. Change in the capability of reactive oxygen species reduction in female judoists production by neutrophils following weight. Br J Sports Med. 2007; 41(5): 322-7.
22) Liesen H, Dufaux B, Hollman W. Modifications of serum glycoproteins the days following a prolonged physical exercise and the influence of physical training. Eur J Appl Physiol Occup Physiol. 1977; 37(4): 243-54.
23) Miles M P, Naukam R J, Hackney A C, Clarkson P M. Blood leukocyte and glutamine fluctuations after eccentric exercise. Int J Sports Med. 1999; 20(5): 322-7.
24) Keast D, Arstein D, Harper W, Fry R W, Morton A R. Depression of plasma glutamine concentration after exercise stress and its possible influence on the immune system. Med J Aust. 1995; 162(1): 15-8.
25) Cruzat V F, Rogero M M, Tirapegui J. Effects of supplementation with free glutamine and the dipeptide alanyl-glutamine on parameters of muscle damage and inflammation in rats submitted to prolonged exercise. Cell Biochem Funct. 2010; 28(1): 24-30.
26) کرمی سجاد، کاشف مجید، گائینی عباسعلی، رجبی حمید، امانی مجید. اثر مصرف مکمل گلوتامین بر تغییرات HSP 72، کورتیزول و گلوکز پلاسما پس از فعالیت ورزشی. نشریۀ غدد درون‌ریز و متابولیسم ایران. 1392؛ 15(2): 73ـ166.
27) Nagashima KCline GWMack GWShulman GINadel ER.. Intense exercise stimulates albumin synthesis in the upright posture. J Appl Physiol. 2000; 88(1): 41-6.
28) Yang R C, Mack G W, Wolfe R R, Nadel E R. Albumin synthesis after intense intermittent exercise in human subjects. J Apply Physiol. 1998; 84(2): 584-92.
29) McNurlan M A, Sandgren A, Hunter K, Essen P, Garlick P J, Wernerman J. Protein synthesis rate of skeletal muscle, lymphocytes, and albumin with stress hormone infusion in healthy man. Metabolism. 1996; 45(11): 1388-94.
30) Lakka A T, Lakka M H, Rankinen T, Rice T, Leon S, Rao D C, et al. Effect of exercise training on plasma levels of C-reactive protein in healthy adults: The heritage family study. European Heart Journal. 2005; 26(19): 2018-25.
31) حقیقی امیرحسین، حامدی‌نیا محمدرضا، جمیلی پرستو. تأثیر تمرینات مقاومتی بر میزان پروتئین واکنش‌پذیر C (CRP) در مردان چاق. نشریۀ دانشکدۀ علوم پزشکی و خدمات بهداشتی درمانی سبزوار. 1385؛ 4(13): 10ـ204.