Document Type : Research Paper
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Abstract
The aim of the present study was investigation of the effects of 6 weeks decreased activity in the form of neuropathic pain on Sunday Driver gene expression in the rat sciatic nerve fibers. Ten adult male Wistar rats in the weight range of 250±30 gr randomly was divided into two groups including healthy control (C), ligation sciatic nerve ligation (SNL). Over the six weeks, neuropathic pain behavior tests were conducted continually in groups. In the end, change of SYD gene expression in sciatic nerve was measured with Real time technique and calculated using the 2-ΔΔCT method and t-test. One- way ANOVA that shown neuropathic pain behavior tests showed that pain threshold of thermal hyperalgesia and mechanical allodynia in the SNL group was significantly lower than the control group (P≤0.05). In addition, SYD gene expression in sciatic nerve ligation group compared to controls increased significantly (P≤0.05).The findings of this study suggest that the development of neuropathic pain and decreased physical activity is associated with increased SYD gene expression. This increase is probably related to the nerve damage and impairment of axonal transport in SNL.
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