Document Type : Research Paper
Authors
Abstract
The aim of this study was to assess the impact of eight weeks aerobic training and omega-3 supplement intake on phosphorylated Tau protein in the hippocampus of Alzheimer induced rats with homocysteine. For this purpose, 60 Wistar male rats (twelve-weeks-old and weight 222.31±11.91 g), divided into six groups, including: Alzheimer’s rat + Omega-3, Alzheimer’s rat + training, Alzheimer’s rat + training + Omega-3, healthy control rat, control sham and Alzheimer’s control rat. Alzheimer's disease induced by injection of homocysteine (60mM) into the rat brain ventricle. Training with a speed of 20 m/min (60 minutes at each session and five days a week on the treadmill) was applied. The supplement group, was received Omega-3 supplement 800mg/kg of body weight, daily for eight weeks. Level of phosphorylated tau protein measured using ELISA method. In data analysis, a one-way analysis of variance and Tukey test as a post hoc, were used )P<0.05). The obtained results showed that the level of phosphorylated Tau protein in hippocampus of Alzheimer's rat + training, Alzheimer’s + Omega-3 and healthy control, were lower than the Alzheimer’s control group (P value 0.03, 0.01 and 0.003 respectively). In addition, there was no significant difference level of phosphorylated tau protein in hippocampus of Alzheimer’s + training + Omega-3 and the control group (P=0.34). It seems that aerobic training and omega-3 supplement, both can be reduced independently phosphorylated tau protein in the hippocampus of Alzheimer's rats and simultaneous use of the two methods, ameliorates the effect each of these interventions
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