Effect of Eighteen Weeks Endurance Training and High-Fat Diet on Amylin Plasma Levels of Male Wistar Rats

Document Type : Research Paper

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Abstract

The purpose of this study was the effect of eighteen weeks’ endurance training and high-fat diet on body weight and amylin plasma levels of male Wistar rats. In order to, 24 young male Wistar rats were divided into three groups (n=8) includes base, control (C) and endurance training (ET) groups in age range 50 to 60 days and average weight 160±10 gr. The ET group rats run on treadmill without slope by 5 day/week, per session 15-65 min, 15-30 m/min velocity for eighteen weeks (incremental). Base group used for initial sampling and C group no train for eighteen weeks. High fat diet was included 60% fat, 24% CHO and 16% protein available two groups; C and ET. Blood sampling was conducted after latest training session with 12h fasting. Data were analyzed using the One-Way ANOVA and Tukey Post-Hoc tests (P<0.05). Data showed that the amylin levels was significantly higher in ET than base groups (P<0.0001). The amylin levels were significantly lower in training than control (no train) group (P<0.0001) and this value was no difference significant in ET than base group (P=0.47). On based of the Lee’s index the body weight was in the area of ​​obesity in control group. The rats' body weight was significantly lower in ET than control group (P<0.0001). Therefore, ingestion of chronic high fat diet leads to the obesity and hyperamylinemia and adding a program of endurance training into this diet prevents with this condition.

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References

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Sport Physiology
Volume 8, Issue 31
Fall 2016
October 2016
Pages 109-120

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History

  • Receive Date: 12 May 2015
  • Revise Date: 29 September 2015
  • Accept Date: 07 November 2015

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