The Effect of High-Intensity Interval Training on Tumor Necrosis Factor-alpha Levels in Visceral and Subcutaneous Adipose Tissue and Insulin Resistance in Male Rats

Kazemi, Abdolreza; Eslami, Rasoul; Karimghasemi, Lotfali

doi:10.22089/spj.2016.860

Document Type : Research Paper

Authors

¹ Assistant Professor in Exercise Physiology, Vali E Asr University of Rafsanjan

² Assistant Professor in Exercise Physiology, Allameh Tabatabai University

³ M.Sc. in Physiology Research Center, Kerman University of Medical Sciences

https://doi.org/10.22089/spj.2016.860

Abstract

The aim of the present study was to investigate the effects of 5 weeks of high-intensity interval training (HIIT) on TNF-α level in visceral and subcutaneous adipose tissue in male Wistar rats. Twenty male rats (age: 8 weeks, weight: 190 ± 10 g) were randomly divided into HIIT training and control groups. Training program consisted of high-intensity speed running. Training program was performed for 5 weeks, 5 sessions per week. In the first week, animals completed 6 high-intensity interval bouts in 2 minutes at 37 m/min with 1-min rest intervals. In the second, third, fourth and fifth weeks the number of bouts increased respectively to 7, 9, 10 and 12, and the speed increased respectively to 40, 43, 49, 52 m/min. Seventy-two hours after the last training session, animals was anesthetized with a cocktail of xialyzine and ketamine, and visceral and subcutaneous adipose was removed. ELISA method was used for TNF-α, glucose and insulin measurement. Independent-samples t test was used for data analysis and significance level was set at 0.05. The results indicated that five weeks of HIIT training caused significant decreases in visceral and subcutaneous levels of TNF-α and plasma insulin (P<0.001) as well as insulin resistance (P<0.01). However, the changes in plasma glucose was not significant (P=0.752). The results of the present study showed that high-intensity interval training decreased TNF-α levels in visceral and subcutaneous adipose tissue and also decreased plasma insulin. Moreover, this type of training can play a role in decreasing insulin resistance.

Keywords

Main Subjects

sport metabolism

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Sport Physiology

The Effect of High-Intensity Interval Training on Tumor Necrosis Factor-alpha Levels in Visceral and Subcutaneous Adipose Tissue and Insulin Resistance in Male Rats

References

References

Volume 8, Issue 32
January 2017
Pages 17-30

The Effect of High-Intensity Interval Training on Tumor Necrosis Factor-alpha Levels in Visceral and Subcutaneous Adipose Tissue and Insulin Resistance in Male Rats

References

References

Volume 8, Issue 32January 2017Pages 17-30

Volume 8, Issue 32
January 2017
Pages 17-30