The Effect of 8 Weeks Aerobic Training After Bone Marrow Stem Cells Transplantation on Striatum VEGF and DA Levels in Parkinson Rats Model

Hashemvarzi, Seyed Abdollah; Heidarianpour, Ali; Fallahmohammadi, Zia; Pourghasem, Mohsen

doi:10.22089/spj.2016.869

Document Type : Research Paper

Authors

¹ Ph.D Student of Exercise Physiology, Bu-Ali Sina University, Hamedan, Iran

² Associate Professor of Exercise Physiology, Bu-Ali Sina University, Hamedan, Iran

³ Associate Professor of Exercise Physiology, University of Mazandaran

⁴ Professor of Cellular and Molecular Research Center, Babol University of Medical Sciences, Mazandaran, Iran

https://doi.org/10.22089/spj.2016.869

Abstract

Stem cells transplantation and exercise training are including of Non-drug treatment option that have been considered for the treatment of Parkinson's disease. The purpose of this study was to investigate the effect of 8 weeks aerobic training on striatum VEGF and DA levels in parkinsonian rats after transplantation of bone marrow stem cells. 35 male rats with the age of 7 weeks and weight 250-300 gr, were divided randomly into healthy control group and parkinsonian groups included of control, cell treatment, Exercise and cell treatment + Exercise. To create a model of Parkinson's, the striatum was destroyed by 6-hydroxy-dopamine injection into the striatum through stereotaxic apparatus and then was used apomorphine rotational test for confirm it. For isolation of bone marrow stem cells, bone marrow of femur and tibia of male rats 6-8 weeks were used. After cultivation, approximately 105 cells in 2 microliter of medium were injected through the channel into the striatum of cell recipient groups. ٍExercise was included 8 weeks of running on the treadmill with a speed of 15 meters per minute in 2 bouts for 5 days in per week. VEGF and DA measured by ELISA method. The results show that, striatum VEGF and DA levels increased significantly in Exercise, Stem cells and specially Stem cells + Exercise groups related to Parkinson group (P≤0.05). Generally, the results of this study approved the positive effect of 8 weeks treadmill exercise in parkinsonian rats after bone marrow stem cell transplantation which can be considered as a non-drug therapeutic manner in Parkinson’s disease.

Keywords

Main Subjects

Neural and muscular

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Sport Physiology

The Effect of 8 Weeks Aerobic Training After Bone Marrow Stem Cells Transplantation on Striatum VEGF and DA Levels in Parkinson Rats Model

References

References

Volume 8, Issue 32
January 2017
Pages 101-114

The Effect of 8 Weeks Aerobic Training After Bone Marrow Stem Cells Transplantation on Striatum VEGF and DA Levels in Parkinson Rats Model

References

References

Volume 8, Issue 32January 2017Pages 101-114

Volume 8, Issue 32
January 2017
Pages 101-114