The Effect of Resistance Training with Skeletal Muscle Hypertrophy on Calcineurin Activation and Expression of IL-6 Myokine in Fast-twitch Skeletal Muscle of Diabetic Rats

Document Type : Research Paper

Author
Mahdieh Molanouri Shamsi
Assistant Professor, Tarbiat Modares University
10.22089/spj.2016.871
Abstract
The phosphatase calcineurin plays a major role in physiological and pathological processes, including immune responses, neuronal plasticity, and skeletal muscle hypertrophy. Considering the effect of this factor in calcium signaling in skeletal muscle, the purpose of the present study was to assess calcineurin activation following hypertrophic resistance training in flexor hallucis longus muscle of diabetic rats with skeletal muscle atrophy. Rats weighing 250–280 g were divided into control, training, diabetic control and diabetic training groups. Training groups performed the training consisting of climbing a ladder (1 m) with increasing weight added to the tail in 17 sessions. The expression levels of interleukin-6 and regulator of calcineurin 1 mRNA, a calcineurin activation factor, were measured in flexor halluces longus muscle using real-time PCR method. The results of this study showed that diabetes was associated with increased expression of regulator of calcineurin 1 in muscles of diabetic rats (P<0.05); but resistance training decreased this factor in diabetic group. Also, diabetes was associated with increased expression of IL-6 in skeletal muscle. Resistance training could not change expression of interleukin-6 in fast twitch skeletal muscle. Diabetes induced skeletal muscle atrophy in rats, but resistance training maintained skeletal muscle mass in diabetic subjects with concurrent adjustment in regulator of calcineurin 1 expression in flexor halluces longus skeletal muscle. Also, we observed concurrent increment in expression of IL-6 and regulator of calcineurin 1 in present study. It seems that resistance training could contribute to skeletal muscle protection through adjustments in expression of these factors.  
Keywords
Type-1 Diabetes
Interleukin-6
Calcineurin
Resistance Training
Skeletal Muscle

Subjects

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Sport Physiology
Volume 8, Issue 32
January 2017
Pages 200-214

  • Receive Date 13 February 2016
  • Revise Date 18 April 2016
  • Accept Date 30 April 2016